LOCUS Exported 9730 bp ds-DNA circular SYN 30-NOV-2021 DEFINITION Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.. ACCESSION . VERSION . KEYWORDS TFORF3180 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9730) AUTHORS Joung J, Zhang F TITLE Multiplexed Overexpression of Regulatory Factors (MORF) Collection JOURNAL unpublished REFERENCE 2 (bases 1 to 9730) AUTHORS . TITLE Direct Submission JOURNAL Exported Nov 30, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9730 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..1179 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 232..1170 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1127..1147 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" regulatory 1190..1199 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" misc_feature 2668..3256 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2721..2741) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3139..3150) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 3328..3561 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 3639..3760 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3676..3695) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3730..3749 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 3800..3935 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(3955..3974) /label=T7 /note="T7 promoter, forward primer" promoter complement(3956..3974) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3984..4001) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(3984..4000) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3993..4015) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 4142..4597 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4229..4248) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4439..4460 /label=F1ori-F /note="F1 origin, forward primer" promoter 4623..4727 /gene="bla" /label=AmpR promoter CDS 4728..5588 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4946..4965) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5759..6347 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6248..6267 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6501..6518 /label=L4440 /note="L4440 vector, forward primer" protein_bind 6635..6656 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 6671..6701 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6709..6725 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6714..6736 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 6733..6749 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 6733..6749 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 6768..6788 /label=T3 /note="T3 promoter, forward primer" promoter 6770..6788 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 6816..7042 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 7043..7223 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 7270..7395 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 7888..8121 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 8306..8350 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 8581..8791 /label=SV40 promoter /note="SV40 early promoter" promoter 8603..8799 /label=SV40 promoter /note="SV40 early promoter" rep_origin 8650..8785 /label=SV40 ori /note="SV40 origin of replication" CDS 8831..9433 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS" primer_bind complement(8831..8850) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 9327..9347 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 9476..9593 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg 61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc 241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt 301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc 1201 caccaataag gagcgactct ttgcggctgg tgccctgggg cctggatctg gctacccagg 1261 ggcaggtttc cccttcgcct tcccaggggc actcaggggg tctccgcctt tcgagatgct 1321 gagccctagc ttccggggcc tgggccagcc tgacctcccc aaggagatgg cctctctgtc 1381 ggtggagaca cagagcacca gctcagagga gatggtgccc agctcgccct cgccccctcc 1441 gcctcctcgg gtctacaagc catgcttcgt gtgcaatgac aagtcctctg gctaccacta 1501 tggggtcagc tcttgtgaag gctgcaaggg cttctttcgc cgaagcatcc agaagaacat 1561 ggtgtacacg tgtcaccgcg acaaaaactg tatcatcaac aaggtgacca ggaatcgctg 1621 ccagtactgc cggctacaga agtgcttcga agtgggcatg tccaaggaag ctgtgcgaaa 1681 tgaccggaac aagaagaaga aagaggtgaa ggaagaaggg tcacctgaca gctatgagct 1741 gagccctcag ttagaagagc tcatcaccaa ggtcagcaaa gcccatcagg agactttccc 1801 ctcgctctgc cagctgggca agtataccac gaactccagt gcagaccacc gcgtgcagct 1861 ggatctgggg ctgtgggaca agttcagtga gctggctacc aagtgcatca tcaagatcgt 1921 ggagtttgcc aagcggttgc ctggctttac agggctcagc attgctgacc agatcactct 1981 gctcaaagct gcctgcctag atatcctgat gctgcgtatc tgcacaaggt acaccccaga 2041 gcaggacacc atgaccttct ccgacgggct gaccctgaac cggacccaga tgcacaatgc 2101 cggcttcggg cccctcacag accttgtctt tgcctttgct gggcagctcc tgcccctgga 2161 gatggatgac accgagacag ggctgctcag cgccatctgc ctcatctgcg gagaccgcat 2221 ggacctggag gagcccgaaa aagtggacaa gctgcaggag ccactgctgg aagccctgag 2281 gctgtacgcc cggcgccggc ggcccagcca gccctacatg ttcccaagga tgctaatgaa 2341 aatcaccgac ctccggggca tcagcactaa gggagctgaa agggccatta ctctgaagat 2401 ggagattcca ggcccgatgc ctcccttaat ccgagagatg ctggagaacc ctgaaatgtt 2461 tgaggatgac tcctcgcagc ctggtcccca ccccaatgcc tctagcgagg atgaggttcc 2521 tgggggccag ggcaaagggg gcctgaagtc cccagcctag taatgaacta gtccgtaact 2581 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa atcgctcgat 2641 atgcaaccga caacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga 2701 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 2761 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 2821 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 2881 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 2941 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 3001 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 3061 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 3121 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 3181 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt 3241 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta 3301 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag 3361 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct 3421 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 3481 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 3541 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt 3601 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata 3661 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 3721 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat 3781 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 3841 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 3901 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat 3961 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac 4021 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat 4081 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg 4141 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 4201 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 4261 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 4321 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 4381 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 4441 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 4501 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 4561 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg 4621 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 4681 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 4741 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 4801 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 4861 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 4921 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 4981 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 5041 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 5101 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 5161 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 5221 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 5281 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 5341 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 5401 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 5461 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 5521 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 5581 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 5641 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 5701 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 5761 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 5821 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 5881 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca 5941 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 6001 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 6061 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 6121 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 6181 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 6241 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 6301 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 6361 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 6421 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 6481 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 6541 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc 6601 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg 6661 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat 6721 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc 6781 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta 6841 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg 6901 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac 6961 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa 7021 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct 7081 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 7141 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 7201 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa 7261 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag 7321 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat 7381 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg 7441 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag 7501 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata 7561 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat 7621 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct 7681 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct 7741 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata 7801 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt 7861 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc 7921 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc 7981 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt 8041 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata 8101 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac 8161 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac 8221 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat 8281 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg 8341 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat 8401 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa 8461 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg 8521 acccatgcat tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 8581 gcaggcagaa gtatgcaaag catgcgtctc aattagtcag caaccatagt cccgccccta 8641 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 8701 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 8761 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttctag aggtaccacc 8821 ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc 8881 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt 8941 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt 9001 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac 9061 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga 9121 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg 9181 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa 9241 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc 9301 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac 9361 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc 9421 cggtgccagt taacgaattc tagatcttga gacaaatggc agtattcatc cacaatttta 9481 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa 9541 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt 9601 attacaggga cagcagagat ccactttggc gccggctcga ggcctgcagg tgcaaagatg 9661 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg 9721 agtgggaatt //