LOCUS Exported 6038 bp ds-DNA circular SYN 01-DEC-2021 DEFINITION Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with HA tag, under UBC promoter.. ACCESSION . VERSION . KEYWORDS pAAV-UBC-XRI-HA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6038) AUTHORS Linghu C, An B, Shpokayte M, Celiker OT, Shmoel N, Zhang C, Park WM, Ramirez S, Boyden ES TITLE Recording of cellular physiological histories along optically readable self-assembling protein chains JOURNAL bioRxiv 2021.10.13.464006 REFERENCE 2 (bases 1 to 6038) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 1, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6038 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 72..201 /label=AAV2 ITR promoter 274..672 /label=UbC promoter /note="human ubiquitin C promoter" CDS 1932..1958 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" polyA_signal 3090..3297 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 3467..3596 /label=AAV2 ITR primer_bind complement(3662..3679) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3833..4421) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3913..3932) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4592..5452) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5215..5234 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5453..5557) /gene="bla" /label=AmpR promoter rep_origin complement(5583..6038) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5720..5741) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 5932..5951 /label=F1ori-R /note="F1 origin, reverse primer" ORIGIN 1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc 61 tattacgcca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 121 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 181 actccatcac taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt 241 agccatgctc taggaagatc gtaggttaat taaggcctcc gcgccgggtt ttggcgcctc 301 ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc 361 gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct 421 tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac 481 tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg 541 cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg 601 cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg 661 atcgtcactt ggcgactagt gccaccatga ttgactatac tgctgctggt ttcaccctcc 721 tccaaggtgc acatctgtac gctcctgaag acagaggtat ctgcgatgtg ctggttgcca 781 acggtaagat catcgctgtg gcctccaaca ttcctagcga catcgttcca aactgcaccg 841 ttgtcgatct gagcggacag atcctctgtc caggattcat tgatcagcac gtgcacctca 901 ttggaggagg aggcgaagct ggtcctacta ctagaactcc cgaagtcgca ctgtctcgcc 961 tcacagaggc aggcgttacc tccgtcgtgg gactcctggg tactgatagc atctctaggc 1021 accctgagtc tctgctggca aagaccagag ccctcaatga ggagggtatc tccgcttgga 1081 tgctgactgg tgcttatcac gtcccatctc gcaccattac tggcagcgtg gagaaggacg 1141 ttgcaatcat cgacagagtg atcggtgtta agtgcgctat ctccgaccac aggagcgctg 1201 ctcccgacgt ctatcacctg gccaacatgg cagccgagag cagagtcggt ggtctgctcg 1261 gtggcaagcc aggagttacc gtgttccaca tgggtgactc taagaaggca ctgcaaccca 1321 tctatgacct gctggagaac tgtgacgtcc caatctccaa gctgctgcct acccacgtca 1381 acaggaacgt gcctctcttc taccaagctc tggagttcgc aagaaagggt ggcaccatcg 1441 acatcacctc tagcattgat gaacccgtcg ctccagctga gggaatcgca agggctgtcc 1501 aagcaggtat cccactggca agggtgacac tcagctctga tggcaacggc tcccagccat 1561 tcttcgatga tgaaggaaat ctgactcaca ttggcgtggc tggatttgaa actctcctgg 1621 aaacagttca ggttctggtt aaggactacg acttcagcat ttctgacgct ctcagaccac 1681 tcacctctag cgtcgctggc ttcctgaacc tgactggtaa aggcgaaatc ctccctggta 1741 atgacgctga tctgctggtg atgacacccg aactgcgcat tgagcaggtc tatgcaagag 1801 gaaagctgat ggttaaggac ggaaaggctt gtgtcaaggg cacattcgag actgctggtg 1861 gctctggagg caccggaggg tctggaggga ctggaggctc tggaggtact ggcggttctg 1921 gaggtaccgg ttacccatac gatgtgcctg attacgcagg atccggtaag attgaagagg 1981 gcaaactggt tatctggatc aatggcgata agggctacaa tggcctggca gaggtcggta 2041 agaagttcga gaaagataca ggcatcaaag ttactgtgga acacccagat aagctggagg 2101 agaagttccc tcaggtcgca gcaactggcg acggtccaga catcatcttc tgggcacacg 2161 ataggttcgg tggctatgcc caatctggcc tgctggctga gattactcct gataaggctt 2221 tccaggacaa gctgtatcca ttcacctggg atgctgtgag atacaatggc aagctgatcg 2281 cataccctat tgctgtggaa gctctgagcc tgatctacaa caaggacctg ctgcctaatc 2341 ctcctaagac atgggaagaa atccctgcac tggacaagga actgaaggcc aaaggcaagt 2401 ccgcactgat gttcaacctg caggagcctt actttacctg gccactgatt gctgccgacg 2461 gtggttacgc tttcaagtac gagaatggta agtacgacat caaagatgtg ggtgtggaca 2521 atgctggtgc caaagctggt ctgactttcc tggtggatct gatcaagaac aagcacatga 2581 atgcagacac tgactattct atcgcagagg ctgctttcaa caaaggcgaa accgcaatga 2641 ctatcaatgg tccttgggca tggtctaaca tcgacactag caaagtcaac tacggtgtca 2701 ccgttctgcc aaccttcaag ggtcagccaa gcaaaccttt cgttggcgtg ctgagcgcag 2761 gtatcaacgc tgcctctcct aacaaagagc tggctaagga gtttctggag aactacctgc 2821 tgacagacga aggtctggag gcagtgaaca aggacaagcc actgggtgcc gtggctctga 2881 agagctacga agaagaactg gccaaggacc ctcgcatcgc tgctacaatg gagaacgcac 2941 agaagggtga gatcatgcca aacatcccac agatgagcgc attctggtat gccgtgagga 3001 ccgctgttat caacgcagct tctggcagac agaccgtgga tgaagccctg aaagacgcac 3061 agacctaagc ggccgctcat aaagctcgac tgtgccttct agttgccagc catctgttgt 3121 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 3181 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 3241 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 3301 ggtccggact gtacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg 3361 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat cttcctagag 3421 catggctacg tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg 3481 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag 3541 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc 3601 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 3661 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 3721 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 3781 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 3841 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 3901 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 3961 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 4021 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 4081 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 4141 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 4201 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 4261 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 4321 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 4381 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 4441 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 4501 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 4561 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 4621 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 4681 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 4741 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 4801 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 4861 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 4921 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 4981 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 5041 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 5101 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 5161 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 5221 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 5281 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 5341 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 5401 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 5461 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 5521 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 5581 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 5641 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 5701 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 5761 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 5821 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 5881 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 5941 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 6001 cacacccgcc gcgcttaatg cgccgctaca gggcgcgt //