LOCUS Exported 7439 bp ds-DNA circular SYN 01-DEC-2021 DEFINITION Conditionally active form of Cre recombinase (activated in response to tamoxifen) under Syn promoter.. ACCESSION . VERSION . KEYWORDS pAAV-Syn-ERT2-iCre-ERT2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7439) AUTHORS Linghu C, An B, Shpokayte M, Celiker OT, Shmoel N, Zhang C, Park WM, Ramirez S, Boyden ES TITLE Recording of cellular physiological histories along optically readable self-assembling protein chains JOURNAL bioRxiv 2021.10.13.464006 REFERENCE 2 (bases 1 to 7439) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 1, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7439 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 58..187 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" polyA_signal 243..291 /note="synthetic polyadenylation signal" misc_feature 305..396 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" primer_bind 345..364 /label=RVprimer3 /note="pGL3 vector, forward primer" promoter 432..879 /label=hSyn promoter /note="human synapsin I promoter; confers neuron-specific expression (Kugler et al., 2003)" regulatory 888..897 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 897..1832 /codon_start=1 /product="mutated ligand-binding domain of the human estrogen receptor (Feil et al., 1997)" /label=ERT2 /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET DQSHLATAGSTSSHSLQKYYITGEAEGFPAT" CDS 2877..3812 /codon_start=1 /product="mutated ligand-binding domain of the human estrogen receptor (Feil et al., 1997)" /label=ERT2 /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET DQSHLATAGSTSSHSLQKYYITGEAEGFPAT" misc_feature 3843..4431 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3896..3916) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4314..4325) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(4464..4481) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4470..4677 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 4686..4815 /label=AAV2 ITR rep_origin 4924..5379 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5011..5030) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5221..5242 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5446..5465) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 5565..5587 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(5625..5643) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 5711..5815 /gene="bla" /label=AmpR promoter CDS 5816..6676 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6034..6053) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6847..7435 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7336..7355 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtcct 61 gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg ggcgtcgggc gacctttggt 121 cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg 181 ggttcctgcg gccgcacgcg ttctagaaat attaaggtac gggaggtact tggagcggcc 241 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag 301 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg 361 tccccagtgc aagtgcaggt gccagaacat ttctctattc tagactcgag ctgcagaggg 421 ccctgcgtat gagtgcaagt gggttttagg accaggatga ggcggggtgg gggtgcctac 481 ctgacgaccg accccgaccc actggacaag cacccaaccc ccattcccca aattgcgcat 541 cccctatcag agagggggag gggaaacagg atgcggcgag gcgcgtgcgc actgccagct 601 tcagcaccgc ggacagtgcc ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc 661 actgaaggcg cgctgacgtc actcgccggt cccccgcaaa ctccccttcc cggccacctt 721 ggtcgcgtcc gcgccgccgc cggcccagcc ggaccgcacc acgcgaggcg cgagataggg 781 gggcacgggc gcgaccatct gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg 841 gtgggcagcg gaggagtcgt gtcgtgcctg agagcgcagt cgaattcgcc accatggctg 901 gagacatgag agctgccaac ctttggccaa gcccgctcat gatcaaacgc tctaagaaga 961 acagcctggc cttgtccctg acggccgacc agatggtcag tgccttgttg gatgctgagc 1021 cccccatact ctattccgag tatgatccta ccagaccctt cagtgaagct tcgatgatgg 1081 gcttactgac caacctggca gacagggagc tggttcacat gatcaactgg gcgaagaggg 1141 tgccaggctt tgtggatttg accctccatg atcaggtcca ccttctagaa tgtgcctggc 1201 tagagatcct gatgattggt ctcgtctggc gctccatgga gcacccagtg aagctactgt 1261 ttgctcctaa cttgctcttg gacaggaacc agggaaaatg tgtagagggc atggtggaga 1321 tcttcgacat gctgctggct acatcatctc ggttccgcat gatgaatctg cagggagagg 1381 agtttgtgtg cctcaaatct attattttgc ttaattctgg agtgtacaca tttctgtcca 1441 gcaccctgaa gtctctggaa gagaaggacc atatccaccg agtcctggac aagatcacag 1501 acactttgat ccacctgatg gccaaggcag gcctgaccct gcagcagcag caccagcggc 1561 tggcccagct cctcctcatc ctctcccaca tcaggcacat gagtaacaaa ggcatggagc 1621 atctgtacag catgaagtgc aagaacgtgg tgcccctcta tgacctgctg ctggaggcgg 1681 cggacgccca ccgcctacat gcgcccacta gccgtggagg ggcatccgtg gaggagacgg 1741 accaaagcca cttggccact gcgggctcta cttcatcgca ttccttgcaa aagtattaca 1801 tcacggggga ggcagagggt ttccctgcca cagctgtcga caacctgctg actgtgcacc 1861 aaaacctgcc tgccctccct gtggatgcca cctctgatga agtcaggaag aacctgatgg 1921 acatgttcag ggacaggcag gccttctctg aacacacctg gaagatgctc ctgtctgtgt 1981 gcagatcctg ggctgcctgg tgcaagctga acaacaggaa atggttccct gctgaacctg 2041 aggatgtgag ggactacctc ctgtacctgc aagccagagg cctggctgtg aagaccatcc 2101 aacagcacct gggccagctc aacatgctgc acaggagatc tggcctgcct cgcccttctg 2161 actccaatgc tgtgtccctg gtgatgagga gaatcagaaa ggagaatgtg gatgctgggg 2221 agagagccaa gcaggccctg gcctttgaac gcactgactt tgaccaagtc agatccctga 2281 tggagaactc tgacagatgc caggacatca ggaacctggc cttcctgggc attgcctaca 2341 acaccctgct gcgcattgcc gaaattgcca gaatcagagt gaaggacatc tcccgcaccg 2401 atggtgggag aatgctgatc cacattggca ggaccaagac cctggtgtcc acagctggtg 2461 tggagaaggc cctgtccctg ggggttacca agctggtgga gagatggatc tctgtgtctg 2521 gtgtggctga tgaccccaac aactacctgt tctgccgggt cagaaagaat ggtgtggctg 2581 ccccttctgc cacctcccaa ctgtccaccc gggccctgga agggatcttt gaggccaccc 2641 accgcctgat ctatggtgcc aaggatgact ctgggcagag atacctggcc tggtctggcc 2701 actctgccag agtgggtgct gccagggaca tggccagggc tggtgtgtcc atccctgaaa 2761 tcatgcaggc tggtggctgg accaatgtga acattgtgat gaactacatc agaaacctgg 2821 actctgagac tggggccatg gtgaggctgc tcgaggatgg ggacctcgag ccatctgctg 2881 gagacatgag agctgccaac ctttggccaa gcccgctcat gatcaaacgc tctaagaaga 2941 acagcctggc cttgtccctg acggccgacc agatggtcag tgccttgttg gatgctgagc 3001 cccccatact ctattccgag tatgatccta ccagaccctt cagtgaagct tcgatgatgg 3061 gcttactgac caacctggca gacagggagc tggttcacat gatcaactgg gcgaagaggg 3121 tgccaggctt tgtggatttg accctccatg atcaggtcca ccttctagaa tgtgcctggc 3181 tagagatcct gatgattggt ctcgtctggc gctccatgga gcacccagtg aagctactgt 3241 ttgctcctaa cttgctcttg gacaggaacc agggaaaatg tgtagagggc atggtggaga 3301 tcttcgacat gctgctggct acatcatctc ggttccgcat gatgaatctg cagggagagg 3361 agtttgtgtg cctcaaatct attattttgc ttaattctgg agtgtacaca tttctgtcca 3421 gcaccctgaa gtctctggaa gagaaggacc atatccaccg agtcctggac aagatcacag 3481 acactttgat ccacctgatg gccaaggcag gcctgaccct gcagcagcag caccagcggc 3541 tggcccagct cctcctcatc ctctcccaca tcaggcacat gagtaacaaa ggcatggagc 3601 atctgtacag catgaagtgc aagaacgtgg tgcccctcta tgacctgctg ctggaggcgg 3661 cggacgccca ccgcctacat gcgcccacta gccgtggagg ggcatccgtg gaggagacgg 3721 accaaagcca cttggccact gcgggctcta cttcatcgca ttccttgcaa aagtattaca 3781 tcacggggga ggcagagggt ttccctgcca cagcttgagc tagcgatatc aagcttatcg 3841 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 3901 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 3961 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 4021 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 4081 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 4141 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 4201 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc 4261 tcgcctgtgt tgccacctgg attctgcgcg ggacgccctt ctgctacgtc ccttcggccc 4321 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 4381 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc 4441 gtcgactaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 4501 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 4561 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 4621 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggagag 4681 atctgaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac 4741 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag 4801 cgagcgagcg cgcagagagg gagtggcccc cccccccccc ccccccctgc agcctggcgt 4861 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 4921 tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 4981 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 5041 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 5101 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 5161 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 5221 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 5281 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 5341 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct 5401 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga 5461 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc 5521 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg 5581 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct 5641 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg 5701 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc 5761 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag 5821 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt 5881 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt 5941 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga 6001 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat 6061 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga 6121 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag 6181 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg 6241 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg 6301 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt 6361 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg 6421 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc 6481 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg 6541 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac 6601 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact 6661 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa 6721 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa 6781 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 6841 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 6901 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 6961 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca 7021 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 7081 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 7141 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 7201 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc 7261 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 7321 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 7381 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacg //