LOCUS Exported 11590 bp ds-DNA circular SYN 09-DEC-2021 DEFINITION lentiviral vector expressing Cas9 and a sgRNA targeting SDHB. ACCESSION . VERSION . KEYWORDS pLentiCRISPRv1_SDHB SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11590) TITLE Fumarate is a terminal electron acceptor in the mammalian electron transport chain REFERENCE 2 (bases 1 to 11590) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 9, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11590 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 123..144 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 159..189 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 197..213 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 202..224 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 221..237 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 221..237 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 256..276 /label=T3 /note="T3 promoter, forward primer" promoter 258..276 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 304..530 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 531..711 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 758..883 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1376..1609 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1794..1838 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 2016..2256 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 2016..2036 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 2187..2206 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 2286..2361 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" misc_feature 2409..2526 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2595..2806 /label=EF-1-alpha core promoter /note="core promoter for human elongation factor EF-1-alpha" regulatory 2826..2835 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2835..2858 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 2865..2885 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 2910..7010 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 7011..7058 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" CDS 7074..7130 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 7131..7730 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(7131..7150) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 7627..7647 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 7746..8334 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7799..7819) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8217..8228) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 8406..8639 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 8717..8838 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8754..8773) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8808..8827 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 8878..9013 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8940..8959 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(9033..9052) /label=T7 /note="T7 promoter, forward primer" promoter complement(9034..9052) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(9062..9079) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(9062..9078) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9071..9093) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 9220..9675 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9307..9326) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9517..9538 /label=F1ori-F /note="F1 origin, forward primer" promoter 9701..9805 /gene="bla" /label=AmpR promoter CDS 9806..10666 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(10024..10043) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10837..11425 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11326..11345 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11579..6 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 61 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 121 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct 181 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat 241 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct 301 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac 361 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg 421 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat 481 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct 541 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 601 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 661 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc 721 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct 781 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 841 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 901 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 961 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 1021 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 1081 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 1141 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 1201 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg 1261 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1321 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1381 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc 1441 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1501 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1561 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1621 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1681 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca 1741 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg 1801 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa 1861 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa 1921 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt 1981 ttcagaccca cctcccaacc ccgaggggac ccagagaggg cctatttccc atgattcctt 2041 catatttgca tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa 2101 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg 2161 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt 2221 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgtcctt tatcacatac 2281 atgtggtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa 2341 aaagtggcac cgagtcggtg cttttttgaa ttctagatct tgagacaaat ggcagtattc 2401 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta 2461 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa 2521 aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct cgagtggctc 2581 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 2641 ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 2701 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 2761 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtgt cgtgacgcgg 2821 gatccgccac catggattac aaagacgatg acgataagat ggccccaaag aagaagcgga 2881 aggtcggtat ccacggagtc ccagcagccg acaagaagta cagcatcggc ctggacatcg 2941 gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat 3001 tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggagccctgc 3061 tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat 3121 acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca 3181 aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga 3241 agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt 3301 accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc 3361 ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg 3421 gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct 3481 acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc 3541 tgtctgccag actgagcaag agcagacggc tggaaaatct gatcgcccag ctgcccggcg 3601 agaagaagaa tggcctgttc ggaaacctga ttgccctgag cctgggcctg acccccaact 3661 tcaagagcaa cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg 3721 acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg 3781 ccgccaagaa cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga 3841 tcaccaaggc ccccctgagc gcctctatga tcaagagata cgacgagcac caccaggacc 3901 tgaccctgct gaaagctctc gtgcggcagc agctgcctga gaagtacaaa gagattttct 3961 tcgaccagag caagaacggc tacgccggct acattgacgg cggagccagc caggaagagt 4021 tctacaagtt catcaagccc atcctggaaa agatggacgg caccgaggaa ctgctcgtga 4081 agctgaacag agaggacctg ctgcggaagc agcggacctt cgacaacggc agcatccccc 4141 accagatcca cctgggagag ctgcacgcca ttctgcggcg gcaggaagat ttttacccat 4201 tcctgaagga caaccgggaa aagatcgaga agatcctgac cttccgcatc ccctactacg 4261 tgggccctct ggccagggga aacagcagat tcgcctggat gaccagaaag agcgaggaaa 4321 ccatcacccc ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc cagagcttca 4381 tcgagcggat gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca 4441 gcctgctgta cgagtacttc accgtgtata acgagctgac caaagtgaaa tacgtgaccg 4501 agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc 4561 tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa 4621 tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg 4681 gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa 4741 acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga 4801 tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga 4861 agcggcggag atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg 4921 acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa 4981 acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc 5041 aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg 5101 ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg 5161 gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga 5221 agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg 5281 gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt 5341 acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc 5401 ggctgtccga ctacgatgtg gaccatatcg tgcctcagag ctttctgaag gacgactcca 5461 tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg caagagcgac aacgtgccct 5521 ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac gccaagctga 5581 ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg 5641 ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca aagcacgtgg 5701 cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag ctgatccggg 5761 aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt 5821 tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg 5881 tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg 5941 actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg 6001 ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc 6061 tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa accggggaga 6121 tcgtgtggga taagggccgg gattttgcca ccgtgcggaa agtgctgagc atgccccaag 6181 tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc 6241 ccaagaggaa cagcgataag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg 6301 gcggcttcga cagccccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg 6361 gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa 6421 gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa 6481 aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga 6541 gaatgctggc ctctgccggc gaactgcaga agggaaacga actggccctg ccctccaaat 6601 atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata 6661 atgagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag atcatcgagc 6721 agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac aaagtgctgt 6781 ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat atcatccacc 6841 tgtttaccct gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg 6901 accggaagag gtacaccagc accaaagagg tgctggacgc caccctgatc caccagagca 6961 tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggcgac aagcgtcctg 7021 ctgctactaa gaaagctggt caagctaaga aaaagaaagc tagcggcagc ggcgccacca 7081 acttcagcct gctgaagcag gccggcgacg tggaggagaa ccccggcccc atgaccgagt 7141 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg 7201 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg 7261 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg 7321 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag 7381 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg 7441 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt 7501 tcctggccac cgtcggagtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg 7561 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg 7621 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg 7681 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga acgcgttaag 7741 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 7801 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 7861 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 7921 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 7981 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 8041 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 8101 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc 8161 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 8221 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 8281 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac 8341 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 8401 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg 8461 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 8521 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 8581 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 8641 tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 8701 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag 8761 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 8821 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc 8881 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 8941 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 9001 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct 9061 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc 9121 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc 9181 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat 9241 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 9301 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 9361 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc 9421 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt 9481 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 9541 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg 9601 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat 9661 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 9721 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 9781 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 9841 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 9901 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 9961 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 10021 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg 10081 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 10141 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 10201 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa 10261 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 10321 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt 10381 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 10441 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 10501 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 10561 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 10621 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 10681 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 10741 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 10801 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 10861 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 10921 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 10981 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 11041 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 11101 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 11161 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 11221 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 11281 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 11341 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 11401 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 11461 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 11521 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 11581 cgagtcagtg //