LOCUS       Exported                7330 bp ds-DNA     circular SYN 15-DEC-2021
DEFINITION  AAV packaging plasmid expressing Rep/Cap genes.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV2/9n
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7330)
  TITLE     AAV packaging plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7330
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     122..144
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     136..153
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     137..153
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     163..182
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        163..181
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     210..226
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     211..227
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4216..4227
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(4679..4699)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4679..4697)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4718..4734)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4718..4734)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4731..4753)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4742..4758
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4766..4796)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4811..4832
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4949..4966)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5120..5708)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5200..5219)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5879..6739)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6502..6521
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6740..6844)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6870..7325)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7007..7028)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7219..7238
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
       61 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
      121 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
      181 ggcgaattgg gtaccgggcc ccccctcgat cgaggtcgac ggtatcgggg gagctcgcag
      241 ggtctccatt ttgaagcggg aggtttgaac gcgcagccgc catgccgggg ttttacgaga
      301 ttgtgattaa ggtccccagc gaccttgacg agcatctgcc cggcatttct gacagctttg
      361 tgaactgggt ggccgagaag gaatgggagt tgccgccaga ttctgacatg gatctgaatc
      421 tgattgagca ggcacccctg accgtggccg agaagctgca gcgcgacttt ctgacggaat
      481 ggcgccgtgt gagtaaggcc ccggaggctc ttttctttgt gcaatttgag aagggagaga
      541 gctacttcca catgcacgtg ctcgtggaaa ccaccggggt gaaatccatg gttttgggac
      601 gtttcctgag tcagattcgc gaaaaactga ttcagagaat ttaccgcggg atcgagccga
      661 ctttgccaaa ctggttcgcg gtcacaaaga ccagaaatgg cgccggaggc gggaacaagg
      721 tggtggatga gtgctacatc cccaattact tgctccccaa aacccagcct gagctccagt
      781 gggcgtggac taatatggaa cagtatttaa gcgcctgttt gaatctcacg gagcgtaaac
      841 ggttggtggc gcagcatctg acgcacgtgt cgcagacgca ggagcagaac aaagagaatc
      901 agaatcccaa ttctgatgcg ccggtgatca gatcaaaaac ttcagccagg tacatggagc
      961 tggtcgggtg gctcgtggac aaggggatta cctcggagaa gcagtggatc caggaggacc
     1021 aggcctcata catctccttc aatgcggcct ccaactcgcg gtcccaaatc aaggctgcct
     1081 tggacaatgc gggaaagatt atgagcctga ctaaaaccgc ccccgactac ctggtgggcc
     1141 agcagcccgt ggaggacatt tccagcaatc ggatttataa aattttggaa ctaaacgggt
     1201 acgatcccca atatgcggct tccgtctttc tgggatgggc cacgaaaaag ttcggcaaga
     1261 ggaacaccat ctggctgttt gggcctgcaa ctaccgggaa gaccaacatc gcggaggcca
     1321 tagcccacac tgtgcccttc tacgggtgcg taaactggac caatgagaac tttcccttca
     1381 acgactgtgt cgacaagatg gtgatctggt gggaggaggg gaagatgacc gccaaggtcg
     1441 tggagtcggc caaagccatt ctcggaggaa gcaaggtgcg cgtggaccag aaatgcaagt
     1501 cctcggccca gatagacccg actcccgtga tcgtcacctc caacaccaac atgtgcgccg
     1561 tgattgacgg gaactcaacg accttcgaac accagcagcc gttgcaagac cggatgttca
     1621 aatttgaact cacccgccgt ctggatcatg actttgggaa ggtcaccaag caggaagtca
     1681 aagacttttt ccggtgggca aaggatcacg tggttgaggt ggagcatgaa ttctacgtca
     1741 aaaagggtgg agccaagaaa agacccgccc ccagtgacgc agatataagt gagcccaaac
     1801 gggtgcgcga gtcagttgcg cagccatcga cgtcagacgc ggaagcttcg atcaactacg
     1861 cagacaggta ccaaaacaaa tgttctcgtc acgtgggcat gaatctgatg ctgtttccct
     1921 gcagacaatg cgagagaatg aatcagaatt caaatatctg cttcactcac ggacagaaag
     1981 actgtttaga gtgctttccc gtgtcagaat ctcaacccgt ttctgtcgtc aaaaaggcgt
     2041 atcagaaact gtgctacatt catcatatca tgggaaaggt gccagacgct tgcactgcct
     2101 gcgatctggt caatgtggat ttggatgact gcatctttga acaataaatg atttaaatca
     2161 ggtatggctg ccgatggtta tcttccagat tggctcgagg acaaccttag tgaaggaatt
     2221 cgcgagtggt gggctttgaa acctggagcc cctcaaccca aggcaaatca acaacatcaa
     2281 gacaacgctc gaggtcttgt gcttccgggt tacaaatacc ttggacccgg caacggactc
     2341 gacaaggggg agccggtcaa cgcagcagac gcggcggccc tcgagcacga caaggcctac
     2401 gaccagcagc tcaaggccgg agacaacccg tacctcaagt acaaccacgc cgacgccgag
     2461 ttccaggagc ggctcaaaga agatacgtct tttgggggca acctcgggcg agcagtcttc
     2521 caggccaaaa agaggcttct tgaacctctt ggtctggttg aggaagcggc taagacggct
     2581 cctggaaaga agaggcctgt agagcagtct cctcaggaac cggactcctc cgcgggtatt
     2641 ggcaaatcgg gtgcacagcc cgctaaaaag agactcaatt tcggtcagac tggcgacaca
     2701 gagtcagtcc cagaccctca accaatcgga gaacctcccg cagccccctc aggtgtggga
     2761 tctcttacaa tggcttcagg tggtggcgca ccagtggcag acaataacga aggtgccgat
     2821 ggagtgggta gttcctcggg aaattggcat tgcgattccc aatggctggg ggacagagtc
     2881 atcaccacca gcacccgaac ctgggccctg cccacctaca acaatcacct ctacaagcaa
     2941 atctccaaca gcacatctgg aggatcttca aatgacaacg cctacttcgg ctacagcacc
     3001 ccctgggggt attttgactt caacagattc cactgccact tctcaccacg tgactggcag
     3061 cgactcatca acaacaactg gggattccgg cctaagcgac tcaacttcaa gctcttcaac
     3121 attcaggtca aagaggttac ggacaacaat ggagtcaaga ccatcgccaa taaccttacc
     3181 agcacggtcc aggtcttcac ggactcagac tatcagctcc cgtacgtgct cgggtcggct
     3241 cacgagggct gcctcccgcc gttcccagcg gacgttttca tgattcctca gtacgggtat
     3301 ctgacgctta atgatggaag ccaggccgtg ggtcgttcgt ccttttactg cctggaatat
     3361 ttcccgtcgc aaatgctaag aacgggtaac aacttccagt tcagctacga gtttgagaac
     3421 gtacctttcc atagcagcta cgctcacagc caaagcctgg accgactaat gaatccactc
     3481 atcgaccaat acttgtacta tctctcaaag actattaacg gttctggaca gaatcaacaa
     3541 acgctaaaat tcagtgtggc cggacccagc aacatggctg tccagggaag aaactacata
     3601 cctggaccca gctaccgaca acaacgtgtc tcaaccactg tgactcaaaa caacaacagc
     3661 gaatttgctt ggcctggagc ttcttcttgg gctctcaatg gacgtaatag cttgatgaat
     3721 cctggacctg ctatggccag ccacaaagaa ggagaggacc gtttctttcc tttgtctgga
     3781 tctttaattt ttggcaaaca aggaactgga agagacaacg tggatgcgga caaagtcatg
     3841 ataaccaacg aagaagaaat taaaactact aacccggtag caacggagtc ctatggacaa
     3901 gtggccacaa accaccagag tgcccaagca caggcgcaga ccggctgggt tcaaaaccaa
     3961 ggaatacttc cgggtatggt ttggcaggac agagatgtgt acctgcaagg acccatttgg
     4021 gccaaaattc ctcacacgga cggcaacttt cacccttctc cgctgatggg agggtttgga
     4081 atgaagcacc cgcctcctca gatcctcatc aaaaacacac ctgtacctgc ggatcctcca
     4141 acggccttca acaaggacaa gctgaactct ttcatcaccc agtattctac tggccaagtc
     4201 agcgtggaga tcgagtggga gctgcagaag gaaaacagca agcgctggaa cccggagatc
     4261 cagtacactt ccaactatta caagtctaat aatgttgaat ttgctgttaa tactgaaggt
     4321 gtatatagtg aaccccgccc cattggcacc agatacctga ctcgtaatct gtaattgctt
     4381 gttaatcaat aaaccgttta attcgtttca gttgaacttt ggtctctgcg aagggcgaat
     4441 tcgtttaaac ctgcaggact agaggtcctg tattagaggt cacgtgagtg ttttgcgaca
     4501 ttttgcgaca ccatgtggtc acgctgggta tttaagcccg agtgagcacg cagggtctcc
     4561 attttgaagc gggaggtttg aacgcgcagc cgccaagccg aattctgcag atatccatca
     4621 cactggcggc cgctcgacta gagcggccgc caccgcggtg gagctccagc ttttgttccc
     4681 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     4741 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     4801 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     4861 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     4921 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4981 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     5041 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     5101 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     5161 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5221 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5281 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     5341 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5401 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5461 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     5521 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     5581 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     5641 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5701 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5761 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     5821 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     5881 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     5941 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     6001 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     6061 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     6121 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     6181 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     6241 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     6301 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     6361 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     6421 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     6481 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     6541 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     6601 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     6661 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     6721 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6781 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6841 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt
     6901 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     6961 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     7021 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     7081 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     7141 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     7201 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     7261 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     7321 cgcgtcccat
//
