LOCUS       Exported                8619 bp ds-DNA     circular SYN 20-DEC-2021
DEFINITION  3rd gen lentiviral eGFP expression vector, CMV promoter, Puro.
ACCESSION   .
VERSION     .
KEYWORDS    pLenti CMV GFP Puro (658-5)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8619)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 8619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8619
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             158..338
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(339..565)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(593..613)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(593..611)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(632..648)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(632..648)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(645..667)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    656..672
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(680..710)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    725..746
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(863..880)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1034..1622)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1114..1133)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1793..2653)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2416..2435
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2654..2758)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2784..3239)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2921..2942)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3133..3152
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3366..3388
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3380..3397
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3381..3397
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3407..3426
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3407..3425
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3446..3581
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3500..3519)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    3621..3742
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3632..3651)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3686..3705
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3814..4047
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(4185..4784)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4268..4288)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4765..4784
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(4805..5304)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4853..4872)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     5246..5269
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    5318..5906
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5424..5435
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5833..5853
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5922..6641)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(5925..6641)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5967..5988)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6316..6335
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6575..6596
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      6638..6647
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(6716..6740)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(6737..6940)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6766..6786)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6941..7244
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    7267..7383
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7650..7694)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7879..8112
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8605..111
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 atctctctcc ttctagcctc cgctagtcaa aatttttggc gtactcacca gtcgccgccc
       61 ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg
      121 tttccctttc gctttcaagt ccctgttcgg gcgccactgc tagagatttt ccacactgac
      181 taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc acacactact
      241 tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag
      301 agctcccagg ctcagatctg gtctaaccag agagacccgt ttatgtatcg agctaggcac
      361 ttaaatacaa tatctctgca atgcggcaat tcagtggttc gtccaatcca tgtcagaccc
      421 gtctgttgcc ttcctaataa ggcacgatcg taccacctta cttccaccaa tcggcatgca
      481 cggtgctttt tctctccttg taaggcatgt tgctaactca tcgttaccat gttgcaagac
      541 tacaagagta ttgcataaga ctacattaag cttgcagctc cagcttttgt tccctttagt
      601 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      661 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
      721 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
      781 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      841 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      901 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      961 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1021 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1081 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1141 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1201 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1261 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1321 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1381 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1441 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1501 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1561 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1621 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1681 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1741 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     1801 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1861 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1921 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1981 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2041 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2101 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2161 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2221 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2281 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2341 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2401 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2461 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     2521 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     2581 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     2641 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2701 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2761 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
     2821 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     2881 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     2941 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     3001 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     3061 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     3121 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     3181 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     3241 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     3301 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     3361 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
     3421 atagggcgaa ttgggtacgt ccctaggcct ccaaaaaagc ctcctcacta cttctggaat
     3481 agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg
     3541 ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg
     3601 atagctagag ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     3661 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     3721 tataagctgc aataaacaag ttcctctcac tctctgatat tcatttcttt gcaagttata
     3781 aatactgaat aataagatga catgaactac tactgctaga gattttccac actgactaaa
     3841 agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa
     3901 gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct
     3961 cccaggctca gatctggtct aaccagagag acccagtaca agcaaaaagc agatcttgtc
     4021 ttcgttggga gtgagctagc ccttccagtc cccccttttc ttttaaaaag tggctaagat
     4081 ctacagctgc cttgtaagtc attggtctta aaggtaccgg gccctcgaga tgcatggggt
     4141 cgtgcgctcc tttcggtcgg gcgctgcggg tcgtggggcg ggcgtcaggc accgggcttg
     4201 cgggtcatgc accaggtgcg cggtccttcg ggcacctcga cgtcggcggt gacggtgaag
     4261 ccgagccgct cgtagaaggg gaggttgcgg ggcgcggagg tctccaggaa ggcgggcacc
     4321 ccggcgcgct cggccgcctc cactccgggg agcacgacgg cgctgcccag acccttgccc
     4381 tggtggtcgg gcgagacgcc gacggtggcc aggaaccacg cgggctcctt gggccggtgc
     4441 ggcgccagga ggccttccat ctgttgctgc gcggccagcc gggaaccgct caactcggcc
     4501 atgcgcgggc cgatctcggc gaacaccgcc cccgcttcga cgctctccgg cgtggtccag
     4561 accgccaccg cggcgccgtc gtccgcgacc cacaccttgc cgatgtcgag cccgacgcgc
     4621 gtgaggaaga gttcttgcag ctcggtgacc cgctcgatgt ggcggtccgg atcgacggtg
     4681 tggcgcgtgg cggggtagtc ggcgaacgcg gcggcgaggg tgcgtacggc cctggggacg
     4741 tcgtcgcggg tggcgaggcg caccgtgggc ttgtactcgg tcatggtaag cttgggctgc
     4801 aggtcgaaag gcccggagat gaggaagagg agaacagcgc ggcagacgtg cgcttttgaa
     4861 gcgtgcagaa tgccgggcct ccggaggacc ttcgggcgcc cgccccgccc ctgagcccgc
     4921 ccctgagccc gcccccggac ccaccccttc ccagcctctg agcccagaaa gcgaaggagc
     4981 aaagctgcta ttggccgctg ccccaaaggc ctacccgctt ccattgctca gcggtgctgt
     5041 ccatctgcac gagactagtg agacgtgcta cttccatttg tcacgtcctg cacgacgcga
     5101 gctgcggggc gggggggaac ttcctgacta ggggaggagt agaaggtggc gcgaaggggc
     5161 caccaaagaa cggagccggt tggcgcctac cggtggatgt ggaatgtgtg cgaggccaga
     5221 ggccacttgt gtagcgccaa gtgcccagcg gggctgctaa agcgcatgct ccagactgcc
     5281 ttgggaaaag cgcctcccct acccggtaga attccaggcg gggaggcggc ccaaagggag
     5341 atccgactcg tctgagggcg aaggcgaaga cgcggaagag gccgcagagc cggcagcagg
     5401 ccgcgggaag gaaggtccgc tggattgagg gccgaaggga cgtagcagaa ggacgtcccg
     5461 cgcagaatcc aggtggcaac acaggcgagc agccatggaa aggacgtcag cttccccgac
     5521 aacaccacgg aattgtcagt gcccaacagc cgagcccctg tccagcagcg ggcaaggcag
     5581 gcggcgatga gttccgccgt ggcaataggg agggggaaag cgaaagtccc ggaaaggagc
     5641 tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc
     5701 acgccacgtt gcctgacaac gggccacaac tcctcataaa gagacagcaa ccaggattta
     5761 tacaaggagg agaaaatgaa agccatacgg gaagcaatag catgatacaa aggcattaaa
     5821 gcagcgtatc cacatagcgt aaaaggagca acatagttaa gaataccagt caatctttca
     5881 caaattttgt aatccagagg ttgattgtcg acgcggccgc tttacttgta cagctcgtcc
     5941 atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc
     6001 tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc gggcagcagc
     6061 acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg
     6121 tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc
     6181 atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg
     6241 tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac
     6301 ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg
     6361 acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag
     6421 cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc
     6481 agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg
     6541 ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg
     6601 ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtggcgac cggtggatcc
     6661 tctagagtcg gtgtcttcta tggaggtcaa aacagcgtgg atggcgtctc caggcgatct
     6721 gacggttcac taaacgagct ctgcttatat agacctccca ccgtacacgc ctaccgccca
     6781 tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc
     6841 aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc
     6901 gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta
     6961 atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc
     7021 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt
     7081 gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag
     7141 tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt
     7201 tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac tcccaagctt
     7261 atcgataaaa ttttgaattt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt
     7321 gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta
     7381 aaagttaacc gataccgtcg agatccgttc actaatcgaa tggatctgtc tctgtctctc
     7441 tctccacctt cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg
     7501 gtctgaaacg ataatggtga atatccctgc ctaactctat tcactataga aagtacagca
     7561 aaaactattc ttaaacctac caagcctcct actatcatta tgaataattt tatataccac
     7621 agccaatttg ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat
     7681 tcttgttcat tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt
     7741 gtgtaattgt taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc
     7801 agagatttat tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca
     7861 gagcaacccc aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt
     7921 cttgcctgga gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca
     7981 atagccctca gcaaattgtt ctgctgctgc actataccag acaataattg tctggcctgt
     8041 accgtcagcg tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg
     8101 aacaaagctc ctattcccac tgctcttttt tctctctgca ccactcttct ctttgccttg
     8161 gtgggtgcta ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc
     8221 caattgtccc tcatatctcc tcctccaggt ctgaagatca gcggccgctt gctgtgcggt
     8281 ggtcttactt ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat
     8341 ctctatcctt tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc
     8401 ttctgatcct gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga
     8461 tgtttctaac aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat
     8521 atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga
     8581 tctaattctc ccccgcttaa tactgacgct ctcgcaccc
//
