LOCUS       Exported               15400 bp ds-DNA     circular SYN 14-MAR-2023
DEFINITION  RUBY under the control of Ubiquitin promoter/marker for 
            transformation.
ACCESSION   .
VERSION     .
KEYWORDS    UBQ:RUBY
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15400)
  AUTHORS   He Y, Zhang T, Sun H, Zhan H, Zhao Y
  TITLE     A reporter for noninvasively monitoring gene expression and plant 
            transformation.
  JOURNAL   Hortic Res. 2020 Sep 19;7:152. doi: 10.1038/s41438-020-00390-1. 
            eCollection 2020.
  PUBMED    33024566
REFERENCE   2  (bases 1 to 15400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 14, 2023 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15400
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    989..1013
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2344..2973
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3402..4475
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      4541..4735
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4971..4993)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5079..5219
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5134..5153
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5234..5251)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5405..5993)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5485..5504)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6239..7030)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    7551..7575
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     7782..7804
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7796..7813
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7797..7813
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      8388..8640
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(9755..9938)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(9791..9970)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             complement(12177..12233)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(13068..13124)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 aggacgagcg gcgtaccttg aagcggaggt gccgacgggt ggatttgggg gagatctggt
       61 tgtgtgtgtg tgcgctccga acaacacgag gttggggaaa gagggtgtgg agggggtgtc
      121 tatttattac ggcgggcgag gaagggaaag cgaaggagcg gtgggaaagg aatcccccgt
      181 agctgccggt gccgtgagag gaggaggagg ccgcctgccg tgccggctca cgtctgccgc
      241 tccgccacgc aagtccaacg gtggagcgga actctcgaga ggggtccaga ggcagcgaca
      301 gagatgccgt gccgtctgct tcgcttggcc cgacgcgacg ctgctggttc gctggttggt
      361 gtccgttaga ctcgtcgacg gcgtttaaca ggctggcatt atctactcga aacaagaaaa
      421 atgtttcctt agttttttta atttcttaaa gggtatttgt ttaattttta gtcactttat
      481 tttattctat tttatatcta aattattaaa taaaaaaact aaaatagagt tttagttttc
      541 ttaatttaga ggctaaaata gaataaaata gatgtactaa aaaaattagt ctataaaaac
      601 cattaaccct aaaccctaaa tggatgtact aataaaatgg atgaagtatt atataggtga
      661 agctatttgc aaaaaaaaag gagaacacat gcacactaaa aagataaaac tgtagagtcc
      721 tgttgtcaaa atactcaatt gtcctttaga ccatgtctaa ctgttcattt atatgattct
      781 ctaaaacact gatattattg tagtactata gattatatta ttcgtagagt aaagtttaaa
      841 tatatgtata aagatagata aactgcactt caaacaagtg tgacaaaaaa aatatgtggt
      901 aattttttat aacttagaca tgcaatgctc attatctcta gagaggggca cgaccgggtc
      961 acgctgcact gcagaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
     1021 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
     1081 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttaaagt
     1141 actttaaagt actttaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct
     1201 tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc
     1261 gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa
     1321 agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg
     1381 gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg
     1441 aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg
     1501 accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga
     1561 ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc
     1621 aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg
     1681 ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca
     1741 tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc
     1801 gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc
     1861 gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac
     1921 ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg
     1981 acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag
     2041 catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc
     2101 ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct
     2161 gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc
     2221 cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt
     2281 gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa
     2341 cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca
     2401 acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat
     2461 ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc
     2521 ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta
     2581 gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac
     2641 ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg
     2701 gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg
     2761 tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac
     2821 gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc
     2881 gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg
     2941 ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca
     3001 aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt
     3061 tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg
     3121 atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat
     3181 ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt
     3241 ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa
     3301 tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg
     3361 ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat
     3421 ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg
     3481 cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag
     3541 cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga
     3601 ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg
     3661 tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg
     3721 accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg
     3781 cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc
     3841 atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt
     3901 tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga
     3961 aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta
     4021 cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc
     4081 gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg
     4141 attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg
     4201 attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg
     4261 caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg
     4321 gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg
     4381 agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca
     4441 acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa
     4501 ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg
     4561 ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca
     4621 ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg
     4681 cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt
     4741 ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc
     4801 tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc
     4861 tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc
     4921 gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     4981 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     5041 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     5101 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     5161 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     5221 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     5281 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     5341 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5401 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5461 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5521 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5581 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5641 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5701 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5761 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5821 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     5881 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5941 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6001 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6061 tggtcatgca tgatatatct cccaatttgt gtagggctta ttatgcacgc ttaaaaataa
     6121 taaaagcaga cttgacctga tagtttggct gtgagcaatt atgtgcttag tgcatctaac
     6181 gcttgagtta agccgcgccg cgaagcggcg tcggcttgaa cgaatttcta gctagacatt
     6241 atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat
     6301 ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta
     6361 tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg
     6421 gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc
     6481 gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct
     6541 caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg
     6601 caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg
     6661 gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct
     6721 tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc
     6781 ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc
     6841 gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg
     6901 gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga
     6961 gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg
     7021 cttcccccat gatgtttaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc
     7081 tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag
     7141 gcatagactg taccccaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt
     7201 taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc
     7261 attacagctt acgaaccgaa caggcttatg tccactgggt tcgtgcccga attgatcaca
     7321 ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt
     7381 ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc
     7441 tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg
     7501 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata
     7561 tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta
     7621 atgtactgaa ttaacgccga attgaattcg agctcggtac ccgccattca ggctgcgcaa
     7681 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     7741 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     7801 aacgacggcc agtgaattcc tcgactcggt accccctcga cacaaaaagc ctatactgta
     7861 cttaacttga ttgcataatt acttgatcat agactcatag taaacttgat tacacagata
     7921 agtgaagaaa caaaccaatt caagacataa ccaaagagag gtgaaagact gttttatatg
     7981 tctaacattg caccttaata tcacactgtt agttcctttc ttacttaaat tcaacccatt
     8041 aaagtaaaaa caacagataa taataatttg agaatgaaca aaaggaccat atcatttatt
     8101 aactcttatc catccatttg cattttgatg tccgaaaaca aaaactgaaa gaacacagta
     8161 aattacaagc agaacaaatg atagaagaaa acagcttttc caatgccata atactcaaac
     8221 ttagtaggat tctggtgtgt gggcaatgaa actgatgcat tgaacttgac gaacgttgtc
     8281 gaaaccgatg atacggacga aagctgggag gcctggatcg atatcaattg acgcgtcgac
     8341 ctgcagccca agctggcatg caagctgtcg aggggggatc aattcccgat ctagtaacat
     8401 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
     8461 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
     8521 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat
     8581 cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc
     8641 ggggaaattc gggggatcag cttgtgatca tgttacatca cctccaagat cccggacgag
     8701 tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg
     8761 ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg
     8821 cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat
     8881 agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct
     8941 ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc
     9001 agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa
     9061 tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca
     9121 cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg
     9181 cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag
     9241 caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg
     9301 ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga
     9361 ccggctgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg
     9421 cacggcggga gatgcaatag gtcaggctct cgctgaattc cccaatgtca agcacttccg
     9481 gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat
     9541 cggcgcagct atttacccgc aggacatatc cacgccctcc tatatcgaag ctgaaagcac
     9601 gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca
     9661 gaaacttctc gacagacgtc gcggtgagtt caggcttttt catatctcat tgccccccgg
     9721 gatcttgcag gtcgagatcc tctagcggat ccggtgcaga ttatttggat tgagagtgaa
     9781 tatgagactc taattggata ccgaggggaa tttatggaac gtcagtggag catttttgac
     9841 aagaaatatt tgctagctga tagtgacctt aggcgacttt tgaacgcgca ataatggttt
     9901 ctgacgtatg tgcttagctc attaaactcc agaaacccgc ggctgagtgg ctccttcaac
     9961 gttgcggttc tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca
    10021 taacgtgact cccttaattc tccgctcatg atcagattgt cgtttcccgc cttcagtttc
    10081 aagcttgttt ggggatctag tgttttactc ctcatattaa cttcggtcat tagaggccac
    10141 gatttgacac atttttactc aaaacaaaat gtttgcatat ctcttataat ttcaaattca
    10201 acacacaaca aataagagaa aaaacaaata atattaattt gagaatgaac aaaaggacca
    10261 tatcattcat taactcttct ccatccattt ccatttcaca gttcgatagc gaaaaccgaa
    10321 taaaaaacac agtaaattac aagcacaaca aatggtacaa gaaaaacagt tttcccaatg
    10381 ccataatact cgaacaagct agcttactca gttaggtctt atctttaatc atattccata
    10441 gtccatacca tagcacatac agtagttata tgctggagaa gagatccaac aaaacattca
    10501 caatggatta tagaaacatt tgtttattca ttataatgag atgttacatt catttaatat
    10561 tagaaaaagc cacaaattca taacacaaca agccaagaaa aaaacacaaa cttaagcaca
    10621 caaccttttt atttgacaca ccaaatattt catcttcatc ttcatattca ctatcactgg
    10681 aggcttggct caagtttggc ctgcatgacg gcgtcgagga aatcgtcgat cgccttcacg
    10741 gaggagccct tcagctcttt ttcctcgttc acggcgtcct tgagcttttt gcccatttcc
    10801 acggcgcgat tcttcatctc ccagcctttg gagccctcgt tgcgctcaag gatcatctcg
    10861 acgatgcgct tgaccttctc tttggagatc tcgccttcga ggcctctagc aagctcaacc
    10921 gcgacgccca tctcatcttc gaggtacttg aggttgtagg cctgctcggc agcaagtggc
    10981 catccaagca tcggaacgcc ctcgcgaaga gactcaagga tgctgttcca tccgcagtgg
    11041 gtgagaaagc cgccgatgga ttcatggttg aggatctcaa gctgcgggcc gagcttgtac
    11101 acgagcttgc cctgtttctt caccttcatg cgttcctcaa agccctctgg cagccactct
    11161 gggcgcatct cttcgttgat gtcgaagccg aacggggctc tgatcaccca gaggaatggc
    11221 ttctcggagg actcaaggcc agcagcgagt tccatcatct gcgtcgggct gatcgtgttc
    11281 tgggagccga aggagatgta gaggacggag tccggctctt tgaggctgag ccactgcacg
    11341 aactcggcgc ctgttgaatt gttgtcggag gaggagtgct gcactgggga agcaataagt
    11401 gggccgatgc cccagatcgg cagcttggtg tagttgcgga ggatggagaa gccgagtgtc
    11461 tcgatctctt ccacggagtt gcagagccag ccaaaagact tcatggactg gcggagctgc
    11521 ggctggaaat acttggacca atcgtcggag ccgtcggcat atctgaggaa ccgatggagc
    11581 tgggacctgc ggaacttatg gttctctggg aagcctggca gcgggaactc ttggtcgtcg
    11641 gagtagttct ggtgcgggag atcgttccag atggacacgt aggcggatgt gccataagcg
    11701 ccgcctgttg tgaacaccac gcctgtagag cccacatcct tggccacctg gtcaacccag
    11761 ccgaggaaca catcatggat cacgcagagt ggtggcctgg ctgggctgaa atgcctggag
    11821 atgtaatcgc ggaggtgcgg atcgaggctg atggtggaat ggaagaggga caccacgagc
    11881 gggagtgtga gcttatcggt gttctcgatg cccggtggga gtccgtggtt ggtggagttg
    11941 aatggcagct cgacgatcct gatgccgctg gaggagtagg aatggtggtg aaggaggtgc
    12001 ctgaggaagc cggcattgag cggagttgtg agcagggtga tgatcacgtg ggagcgcttg
    12061 tagaggaaca tggcgagttc gagaaatggc ctgaggtggc cctgcgccat gaacgggatc
    12121 atgaggatgt gctgtgtctc gccctcgccg ttggtgttca tcttgatggc ggtcatgggg
    12181 ccaggatttt cctcaacatc tcctgcttgc ttgagcaggc taaagttggt tgctccggat
    12241 ccggcggagg tgaacttgta ggagccgtgg caaagtgtgc catgatccca ggaggagtgg
    12301 atcagctcgg ccttccactt ttcaccagca gcgccgagca caacgtggag cgggtagaag
    12361 tgctctggaa atggatgggc gagcttccag ttcggggcct tgctctcgta gatgttcact
    12421 tcctcgaagc ggccgttgat gagggccttc ctaagccaag aatcgaaggc agcggcccaa
    12481 ggggcaacac catcgaagta gtgcggtgtc tcatcgagtg gatgtgtggc gctgccggag
    12541 ccaataatga gcacgccatc attcttgagc ggcgcgaggg ctctgccgag attgtagtgg
    12601 taggtgccgt cgagatgtgg ctgaactgag agctggcaca ccgggatgtc cgcctctgga
    12661 tacatgagca tgagtggaac ccaggcgccg tgatcaaggc ccctcttttg gtctgtctcg
    12721 gctgtctcga acccggactt cttgaggatc tcttccacct ttctcgcaag ctctggctcg
    12781 cctggagctg ggtacttgaa ctggtacatg gcggctgggt agtcgtcgaa gtcgtggatg
    12841 gtgtcgttga tgtgcacggc gttcacggtt ggcttcactg tctcccagtg gccggagatg
    12901 atgaggatgg ccttcggctt cttggagaaa atcttctcgc gccatgtctc gaagaacggc
    12961 ctgagcggat gtgtatcctc gacggtgagg atcgggttgc cgtgggtgat gaagaaggac
    13021 tctttgatca tctggtcgtt ggcgtcctcg ccgttcatca tcttcatagg cccggggttc
    13081 tcttctacat cacctgcctg cttaaggagg ctaaaattgg tagctccgct accgtagcgc
    13141 ggaatcggga tgagcttgag cggcttggtc ttttggagcg caatgccgaa cttctcgtcc
    13201 atgtcgaggt ccttcgggga gatgtcgcct tcgagcttcc agttgaagaa ctggaggagt
    13261 gtggcgagca tgagggtgag catcctgatg gcgagattca tgcctgggca aattctgcgg
    13321 ccagcgccaa atggaaggag gccgaaatcg cggcccttaa catcgatctc gcagccgatg
    13381 aagcgctctg ggctgaaaat atcggcgttc tgccaggcgt ttgggtccct gccaatggcc
    13441 cagaggttca cgaggatctg ggcatccttc ggcacgatgt agccgtacag ctcgacatcg
    13501 gtgtcggcct ttcttgggag caagaacacg gtcggcggat ggaggcggag tgtctctttg
    13561 atgatcgcct ggaggtacgg caggttgatg atgtcggact cttggatctg cttgtccttg
    13621 ccgaggacct gcttgatttc ctcttgggcc ttttccatca tctctggatt gcggatcagc
    13681 tcggtcatca cccactcgaa tgtggaggat gtggtatcgg tgccggcgtc gaagatgtcc
    13741 acgaggaggt ggttgatctc gcccatcgtc agctcgttct gcttgaacag ctggaggagc
    13801 acatcgagca cgtcgtcggt ggtggtggta gttgtggtgg aggaatctgg ggcgagtctc
    13861 tcgcagatga tgccctggaa cacggcgatc agcttgtcga aggagcaggc gagccttctt
    13921 ctaatgccag atgggtcaac gcagccgaga atcgggaagt agtcggcgta gttcggcttg
    13981 cctatatctt ccatgatgtt ccagatcagc tctttgaact cttggctggt atgggacttg
    14041 tggtgcgcca gctcgacgct gaagaacagc ttgctgagga ggttgaggct ggtcgtaaag
    14101 gcggccttgc caatatccac ggcctggcct ttctgggcgc actcttgaac gtactcgtag
    14161 agctgctgca ccttggcgtg cctgaatgtc tggcaggcat cgagtctctg tggggagagc
    14221 agatgcacgg cggtaatctt gcggaagttg cgccacttcg gagacaccgg gagccaagac
    14281 atggtgagct tgtggtggtc gccggctgta acactattcg ggatcgtgcg gttggagagc
    14341 gggtgatcct tcttgaggaa catctctttg gccacgtcgg cagaggacac aacgatggtg
    14401 gtcacagagc cgagtctgag ggaaatgagt gggccgtgaa tcttggcgag gttggcgaag
    14461 gaccgatgcg gcttcttgcc cacctcaagg atgttgccga tgatcggaag cggctttggt
    14521 cctggcggaa gcagcttggt ggtctgctgg gagaacagca gcttgatgaa gtggaagctg
    14581 atgaaccaga tcgcgaggat catggcgagg gtcgcatgat ccatactagt ttctgcagaa
    14641 gtaacaccaa acaacagggt gagcatcgac aaaagaaaca gtaccaagca aataaatagc
    14701 gtatgaaggc agggctaaaa aaatccacat atagctgctg catatgccat catccaagta
    14761 tatcaagatc aaaataatta taaaacatac ttgtttatta taatagatag gtactcaagg
    14821 ttagagcata tgaatagatg ctgcatatgc catcatgtat atgcatcagt aaaacccaca
    14881 tcaacatgta tacctatcct agatcgatat ttccatccat cttaaactcg taactatgaa
    14941 gatgtatgac acacacatac agttccaaaa ttaataaata caccaggtag tttgaaacag
    15001 tattctactc cgatctagaa cgaatgaacg accgcccaac cacaccacat catcacaacc
    15061 aagcgaacaa aaagcatctc tgtatatgca tcagtaaaac ccgcatcaac atgtatacct
    15121 atcctagatc gatatttcca tccatcatct tcaattcgta actatgaata tgtatggcac
    15181 acacatacag atccaaaatt aataaatcca ccaggtagtt tgaaacagaa ttctactccg
    15241 atctagaacg accgcccaac cagaccacat catcacaacc aagacaaaaa aaagcatgaa
    15301 aagatgaccc gacaaacaag tgcacggcat atattgaaat aaaggaaaag ggcaaaccaa
    15361 accctatgca acgaaacaaa aaaaatcatg aaatcgatcc
//
