LOCUS Exported 4726 bp ds-DNA circular SYN 20-DEC-2021 DEFINITION Localization: N1 Cloning Vector, Excitation: 559, Emission: 600. ACCESSION . VERSION . KEYWORDS mRuby2-N1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4726) AUTHORS Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin MZ TITLE Improving FRET dynamic range with bright green and red fluorescent proteins. JOURNAL Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171. PUBMED 22961245 REFERENCE 2 (bases 1 to 4726) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 20, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4726 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 65..422 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(88..108) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 273..408 /label=SV40 ori /note="SV40 origin of replication" primer_bind 335..354 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 457..1251 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(511..530) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 1121..1140 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(1439..1458) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 1483..1530 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 1859..2447 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2348..2367 /label=pBR322ori-F /note="pBR322 origin, forward primer" enhancer 2622..2925 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2926..3129 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 3080..3100 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" misc_feature 3152..3232 /label=MCS /note="multiple cloning site" regulatory 3234..3243 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3240..3950 /codon_start=1 /product="monomeric red fluorescent protein derived from mRuby, with improved photophysical properties (Lam et al., 2012)" /label=mRuby2 /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV AKFAGLGGGMDELYK" polyA_signal 4075..4196 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4112..4131) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4166..4185 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(4203..4658) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4340..4361) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 4552..4571 /label=F1ori-R /note="F1 origin, reverse primer" promoter 4685..63 /gene="bla" /label=AmpR promoter ORIGIN 1 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 61 agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc 121 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 181 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 241 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 301 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 361 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 421 aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac 481 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca 541 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt 601 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg 661 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga 721 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct 781 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg 841 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg 901 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc 961 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat 1021 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac 1081 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt 1141 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct 1201 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc 1261 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca 1321 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 1381 tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg 1441 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga 1501 ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc 1561 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt 1621 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg 1681 caggccctgc catagcctca ggttactcat atatacttta gattgattta aaacttcatt 1741 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 1801 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 1861 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 1921 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 1981 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca 2041 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 2101 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 2161 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 2221 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 2281 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 2341 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 2401 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 2461 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 2521 tatcccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca 2581 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta 2641 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat 2701 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg 2761 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac 2821 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt 2881 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg 2941 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc 3001 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt 3061 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 3121 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc 3181 tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg gtcgccacca 3241 tggtgtctaa gggcgaagag ctgatcaagg aaaatatgcg tatgaaggtg gtcatggaag 3301 gttcggtcaa cggccaccaa ttcaaatgca caggtgaagg agaaggcaat ccgtacatgg 3361 gaactcaaac catgaggatc aaagtcatcg agggaggacc cctgccattt gcctttgaca 3421 ttcttgccac gtcgttcatg tatggcagcc gtacttttat caagtacccg aaaggcattc 3481 ctgatttctt taaacagtcc tttcctgagg gttttacttg ggaaagagtt acgagatacg 3541 aagatggtgg agtcgtcacc gtcatgcagg acaccagcct tgaggatggc tgtctcgttt 3601 accacgtcca agtcagaggg gtaaactttc cctccaatgg tcccgtgatg cagaagaaga 3661 ccaagggttg ggagcctaat acagagatga tgtatccagc agatggtggt ctgaggggat 3721 acactcatat ggcactgaaa gttgatggtg gtggccatct gtcttgctct ttcgtaacaa 3781 cttacaggtc aaaaaagacc gtcgggaaca tcaagatgcc cggtatccat gccgttgatc 3841 accgcctgga aaggttagag gaaagtgaca atgaaatgtt cgtagtacaa cgcgaacacg 3901 cagttgccaa gttcgccggg cttggtggtg ggatggacga gctgtacaag taagcggccg 3961 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac 4021 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg 4081 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 4141 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc 4201 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 4261 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 4321 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 4381 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 4441 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 4501 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 4561 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 4621 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa 4681 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaat //