LOCUS Exported 8042 bp ds-DNA circular SYN 22-DEC-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS HA-HIF2alpha-P405A/P531A-pcDNA3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8042) AUTHORS Yan Q, Bartz S, Mao M, Li L, Kaelin WG TITLE The hypoxia-inducible factor 2alpha N-terminal and C-terminal transactivation domains cooperate to promote renal tumorigenesis in vivo. JOURNAL Mol Cell Biol. 2007 Mar . 27(6):2092-102. PUBMED 17220275 REFERENCE 2 (bases 1 to 8042) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 22, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8042 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(121..450) /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 135..270 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(189..208) /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind 435..455 /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin complement(464..892) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(574..595) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 786..805 /label=F1ori-R /note="F1 origin, reverse primer" polyA_signal 938..1162 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind 1151..1168 /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" promoter 1188..1206 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 1188..1205 /label=SP6 /note="SP6 promoter, forward primer" CDS complement(3871..3897) /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" primer_bind complement(3919..3938) /label=T7 /note="T7 promoter, forward primer" promoter complement(3920..3938) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter complement(3983..4186) /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind complement(4012..4032) /label=CMV-F /note="Human CMV immediate early promoter, forward primer" enhancer 4187..4566 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" primer_bind 4738..4757 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" promoter 4832..4936 /gene="bla" /label=AmpR promoter CDS 4937..5797 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5155..5174) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5968..6556 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6457..6476 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6710..6727 /label=L4440 /note="L4440 vector, forward primer" protein_bind 6844..6865 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 6880..6910 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6918..6934 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6923..6945 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 6942..6958 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 6942..6958 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" polyA_signal 7007..7128 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7018..7037) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 7072..7091 /label=SV40pA-R /note="SV40 polyA, reverse primer" CDS complement(7302..54) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(7413..7432) /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind 8023..8042 /label=Neo-R /note="Neomycin resistance gene, reverse primer" ORIGIN 1 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa 61 cgatcctcat cctgtctctt gatcagatcc gaaaatggat atacaagctc ccgggagctt 121 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc 181 agaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg 241 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc 301 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc 361 acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc 421 tggggacttt ccacacccta actgacacac attccacaga attaattcgc gttaaatttt 481 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca 541 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta 601 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta 661 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg 721 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga 781 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg 841 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtggggatac 901 cccctagagc cccagctggt tctttccgcc tcagaagcca tagagcccac cgcatcccca 961 gcatgcctgc tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc 1021 agaatagaat gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg 1081 acagtgggag tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat 1141 ggctggcaac tagaaggcac agtcgaggct gatcagcgag ctctagcatt taggtgacac 1201 tatagaatag ggccctctag atgcatgctc gagcggccgc aattgtcagg tggcctggtc 1261 cagggctctg aggaggtccc ctccttgcag gagcgtggag cttcccagca cgggcacgtt 1321 cacctcacag tcatatctgg tcagttcggg cagcaggtag gactcaaatg agggcccgag 1381 cagccggctt gccatgcctg acaccttgtg ggctgacgac aggctgtagt cctggtactg 1441 ggtggcgtag cactgtgggg ggaacctgct cttgctgttc tccccgggac tgatggcaga 1501 tggaggctgt ggcagcggca gatgtctcag gggatggccc aggcccctca tggggttttg 1561 ggtgaactta tcattgggta catttgcgct cagtggcttg tccggcatca aagggcagct 1621 cccacccctg aggttcttca tccgtttcca catcaaatgt gaggtgctgc caccaggtgg 1681 gtcccccccg ctcaggtcct ggaaggcttg ctcttcatac tccagctgtc gcttcagctt 1741 cagcttgttg gagagggcta ccatggccgg actcagcacg tctgggcctc gagccccaaa 1801 accctttgca gaccttgtct tgaaggtgga gacatggggt ggagagacag ggggccccaa 1861 cggcgctgct cccaagaact ctgtgcgctg atccccgacg gcccactttg tgggcccaaa 1921 atgtaatggt ggatctgggg gccactgggt attggatctg ccccccatgg aagagagagg 1981 ggtgctggcc tggccacagc acggtggcag ggatgctttg cttccggcat caaagaagat 2041 ggaggacatg ggccggtgct cgggctctgt cttcttgctc tccagctgct gctgaaactt 2101 gtccaggagg aagggactgt gcggggctac aggggccagt ggctggaaga tgtttgtcat 2161 ggcactgaag cagtgctggg gggtggactg tgggttctcc gccaagagcc gctcctcggg 2221 gcagatgggg cttagctgga agtcttcccc gtccatgggg atataagctg ccagtgtctc 2281 caagtccagc tcattgaaat ccgtctgggt actgcattgg tccttggcct ctgtgtccat 2341 ggcgaagagc ttctcaatca cttcaatctt caggtcgtta tccaaagatg tgtaatagtc 2401 ttcagggcta ttgggcgtgg agcagctgct gctgctgctg gtggcactgg gggtggtgct 2461 gcccggggca gctgcctggg gcacggtgaa ggcaggcagg ctcccagcct cgctctgggt 2521 gctgtggctc ctcaactccg tggcccatgg ctggctcggg ggcaggatgg ccttgccata 2581 ggctgaggac tcctcgaagt tctgattccc gaaatccaga gagatgatgg cgtctcctgg 2641 ggtagcagcc agctgggcca gctcctcggg ctcctccttt agcttggtga ataggaagtt 2701 actcttctca gacacagccc ccttgccact gctatcaaag atgctgttca tggccatcag 2761 gtggggcttg aacagggatt cagtctggtc catggagaac accacgtcat tcttctcaat 2821 ctcactcagg acgtagttga cacacatgat gcactggggc tgcaggttgc gagggttgta 2881 gatgaccgtc ccctgggtct ccagccacac gtagccccca tgctttgcga gcatccggta 2941 ctggccactt actacctgac ccttggtgca caagttctgg tgactcttgg tcatgttctc 3001 ggagtctagc gcatggtaga attcataggc tgagcggcca agcagctcct cagggtggta 3061 accaatcagt tctgtgattc tgtcatcaca gtaggtgaac ttcatgtcca tgctgtggcg 3121 gctcaggaag gtcttgctat ccagggggat gtccatgtgg gatgggtgct ggattggttc 3181 acacatgatg atgaggcagg acagcagggg ctccttgtag ccacacagac tattgtgagg 3241 agggcagttg ttgtagactt tcacctggcc cgtgcagtgc aagaccttcc aggtggctga 3301 cttgaggttg acagtacggc ctctgttggt gaccgtgcac ttcatcctca tgaagaagtc 3361 ccgctctgtg gacatgtctt tgcttttttt cccaaaacca gagccatttt tgagactcag 3421 gttctcacga atctcctcat ggtcgcaggg atgagtgaag tcaaagatac tatgtcctgt 3481 tagctccacc tgtgtaagtc ccatgaactt gctgatgttt tctgacagaa agatcatgtc 3541 gccatcttgg gtcaccacgg caatgaaacc ctccaaggct ttcaggtaca agttgtccat 3601 ctgctggtca gcttcggctt cggactcgtt ttcagagcaa actgaggaga ggagcttgtg 3661 tgttcgcagg aagctgattg ccagtcgcat gatggaggcc ttgtccagat gggagctcac 3721 actgtggggc agaggcagct catgggccag ctcatagaac acctccgtct ccttgctccg 3781 ccggcaccgc gcagcatccc gggacttctc cttcctcctc tccgagctac tccttttctt 3841 ctccttgtca gctgtcatgg tggcggatcc agcataatct ggaacatcat atggatacat 3901 ggtggcaagc ttgggtctcc ctatagtgag tcgtattaat ttcgataagc cagtaagcag 3961 tgggttctct agttagccag agagctctgc ttatatagac ctcccaccgt acacgcctac 4021 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt 4081 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt 4141 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga 4201 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat 4261 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat 4321 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat 4381 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg 4441 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca 4501 tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta gtcaataatc 4561 aatgtcaacg cgtatatctg gcccgtacat cgcgaagcag cgcaaaacgc ctaaccctaa 4621 gcagattctt catgcaattg tcggtcaagc cttgccttgt tgtagcttaa attttgctcg 4681 cgcactactc agcgacctcc aacacacaag cagggagcag atactggctt aactatgcgg 4741 catcagagca gattgtactg agagtgcacc ataggggatc gggagatctc ccgatccgtc 4801 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 4861 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 4921 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 4981 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 5041 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 5101 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 5161 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 5221 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 5281 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 5341 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 5401 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 5461 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 5521 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 5581 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 5641 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 5701 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 5761 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 5821 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 5881 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 5941 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 6001 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 6061 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct 6121 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 6181 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 6241 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 6301 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 6361 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 6421 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 6481 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 6541 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 6601 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 6661 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 6721 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 6781 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 6841 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc 6901 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca 6961 tgattacgcc aagctctagc tagaggtcga cggtatacag acatgataag atacattgat 7021 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt 7081 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttgg ggtgggcgaa 7141 gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat 7201 tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt 7261 gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga 7321 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag 7381 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc 7441 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt 7501 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg 7561 ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg 7621 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga 7681 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt 7741 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc 7801 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca 7861 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt 7921 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac 7981 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat 8041 ag //