LOCUS       Exported                5613 bp ds-DNA     circular SYN 23-JAN-2024
DEFINITION  Cre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    paavCAG-iCre
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Druckmann S, Feng L, Lee B, Yook C, Zhao T, Magee JC, Kim J
  TITLE     Structured Synaptic Connectivity between Hippocampal Regions.
  JOURNAL   Neuron. 2014 Feb 5;81(3):629-40. doi: 10.1016/j.neuron.2013.11.026. 
            Epub 2014 Jan 9.
  PUBMED    24412418
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 23, 2024 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   27..156
                     /label=AAV2 ITR
     primer_bind     1025..1047
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1109..1128
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1170..1190
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    2232..2820
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2285..2305)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2703..2714)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2863..2984
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2874..2893)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2928..2947
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(3363..3951)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3443..3462)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4122..4982)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4745..4764
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4983..5087)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5113..5568)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5250..5271)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5462..5481
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgcgggcctc ttcgctatta cgccagctgc gcgctcgctc gctcactgag gccgcccggg
       61 caaagcccgg gcgtcgggcg acctttggtc gcccggcctc agtgagcgag cgagcgcgca
      121 gagagggagt ggccaactcc atcactaggg gttccttgta gttaatgatt aacccgccat
      181 gctacttatc tacgtagcca tgctctagga agatcgtacc attgacgtca ataatgacgt
      241 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      301 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      361 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      421 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc
      481 ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat
      541 ttatttttta attattttgt gcagcgrkgg gggsgggggg gggggggggg sgsgcscsrg
      601 gsggggsggg gsggggsgrg gggsggggsg gggsgrggsg grgrggkgsg gsggcrgcca
      661 awcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct
      721 ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc
      781 gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg
      841 tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc
      901 ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg
      961 gggggagcgg ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg
     1021 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat
     1081 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
     1141 tttggcaaag aattggatcc accatggtgc ccaagaagaa gaggaaagtc tccaacctgc
     1201 tgactgtgca ccaaaacctg cctgccctcc ctgtggatgc cacctctgat gaagtcagga
     1261 agaacctgat ggacatgttc agggacaggc aggccttctc tgaacacacc tggaagatgc
     1321 tcctgtctgt gtgcagatcc tgggctgcct ggtgcaagct gaacaacagg aaatggttcc
     1381 ctgctgaacc tgaggatgtg agggactacc tcctgtacct gcaagccaga ggcctggctg
     1441 tgaagaccat ccaacagcac ctgggccagc tcaacatgct gcacaggaga tctggcctgc
     1501 ctcgcccttc tgactccaat gctgtgtccc tggtgatgag gagaatcaga aaggagaatg
     1561 tggatgctgg ggagagagcc aagcaggccc tggcctttga acgcactgac tttgaccaag
     1621 tcagatccct gatggagaac tctgacagat gccaggacat caggaacctg gccttcctgg
     1681 gcattgccta caacaccctg ctgcgcattg ccgaaattgc cagaatcaga gtgaaggaca
     1741 tctcccgcac cgatggtggg agaatgctga tccacattgg caggaccaag accctggtgt
     1801 ccacagctgg tgtggagaag gccctgtccc tgggggttac caagctggtg gagagatgga
     1861 tctctgtgtc tggtgtggct gatgacccca acaactacct gttctgccgg gtcagaaaga
     1921 atggtgtggc tgccccttct gccacctccc aactgtccac ccgggccctg gaagggatct
     1981 ttgaggccac ccaccgcctg atctatggtg ccaaggatga ctctgggcag agatacctgg
     2041 cctggtctgg ccactctgcc agagtgggtg ctgccaggga catggccagg gctggtgtgt
     2101 ccatccctga aatcatgcag gctggtggct ggaccaatgt gaacattgtg atgaactaca
     2161 tcagaaacct ggactctgag actggggcca tggtgaggct gctcgaggat ggggactgaa
     2221 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     2281 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     2341 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     2401 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     2461 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     2521 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     2581 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     2641 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     2701 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     2761 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     2821 atcgataccg tcgacccggg cggccgcttc gagcagacat gataagatac attgatgagt
     2881 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     2941 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     3001 ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaagc aagtaaaacc
     3061 tctacaaatg tggtaaaatc gataaggatc ttcctagagc atggctacgt agataagtag
     3121 catggcgggt taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct
     3181 ctkcncgcty sctcgctcac tgaskcsskg cgmycrrwcg ttcggctgcg gcgagcggta
     3241 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     3301 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3361 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3421 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3481 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3541 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3601 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3661 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     3721 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     3781 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     3841 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3901 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3961 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4021 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4081 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     4141 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     4201 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     4261 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     4321 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     4381 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     4441 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     4501 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     4561 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     4621 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     4681 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     4741 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     4801 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     4861 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     4921 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     4981 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     5041 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     5101 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     5161 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     5221 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     5281 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     5341 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     5401 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     5461 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     5521 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat
     5581 tcaggctgcg caactgttgg gaagggcgat cgg
//
