LOCUS       Exported               13173 bp ds-DNA     circular SYN 05-JAN-2022
DEFINITION  Pmyo-3 DAF-2 unc-54 3' UTR C.elegans muscle expression of DAF-2 .
ACCESSION   .
VERSION     .
KEYWORDS    pJH4733
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13173)
  AUTHORS   Hung WL, Wang Y, Chitturi J, Zhen M
  TITLE     A Caenorhabditis elegans developmental decision requires insulin 
            signaling-mediated neuron-intestine communication.
  JOURNAL   Development. 2014 Apr;141(8):1767-79. doi: 10.1242/dev.103846. Epub 
            2014 Mar 26.
  PUBMED    24671950
REFERENCE   2  (bases 1 to 13173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 5, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13173
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    114..135
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        150..180
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    188..204
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     193..215
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     212..228
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     212..228
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10309..10326)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10309..10325)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10318..10340)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10538..10993
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10625..10644)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10835..10856
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11010..11029)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11129..11151
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11189..11207)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11275..11379
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11380..12240
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11598..11617)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12411..12999
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12900..12919
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13153..13170
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
       61 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
      121 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
      181 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat
      241 tcgaatggcc atgggacgtc gacgcttgca tgcctgcagc gtttccgata cccccttata
      301 tcagcacaca ttcagtcatc ataccacaaa atgttgtgat agtgataggc aaaactaccg
      361 tatttcctct attaatcttg catgcaagac taattttcga ttaacccgta ggggtgcaag
      421 actaatagag actgcaagac tattagaggc tgaaatacta attttcgtat gctcaataat
      481 tttggaaatt ggcctatttt ttgtagaaac ttgataccgt ttgcggccgc cggctataat
      541 aagttctgaa taaaataatt ttcccgacaa aacatgagta tttctttcga aaataaaagt
      601 gcaggctaat tagagattat tctgtaatta actgcataat ttgtcacgtg ccatagtttt
      661 acattccact acgtcatagt tcttaaaata ctaatctcct gaaaatagaa gtaggtgaag
      721 aaagtttaat tatcagttct aaaatgacaa ttgatctttg gaatatgttc tgaaactacc
      781 gatcattgaa cagatgctat ttgaatgata tagaattgta tatttgcaat ttctgaaacg
      841 cgttcttaaa ggcacacaga ttaattcaaa agggtctggc cgcaaaaagg tttatggtgg
      901 ccgattttga gttttgtgtg tgattgcttt ttcacaatca gtgttttcag gattatgtga
      961 tgaactagat cttcaagttt cgttacattt catatgtttt cggaactcac gaagtacata
     1021 ttgggtattg tgctcaaaaa attcagcaat cagcttcgct ccgctgactt tagaacccaa
     1081 aaaaatagta tggccaaact gactgtgtta cgatcatttc aatttttcaa tacatattta
     1141 agatttctaa gagtaagaag gtcaaaaact gttctggaat acatatatat ttttcaggtt
     1201 acaattagtc aaaaagtgca ctgaaatata cgttttaatt tcacgaataa cccaattagt
     1261 tcaatgtatt tttggtcaac caacgttaaa gtttggcttc caaccaatta tcatttctga
     1321 tcaaccacaa tgttttttct ttatctgcaa gttaattttt tatttttatc cagatgtttg
     1381 gcatattttt caattcttca ctagcgccca cttcttgcac ttccggcgcc ctgaatctaa
     1441 tgcatctgtt gcaagaattg aaagaccaat caacacattg ttttcttcac gagatactga
     1501 agaaaatgaa taaaaacaga gaaaaagagc catgtgatta gtgacaactg ttgctaacag
     1561 ataccatagc ttggacttgg tacgtgatgg caacgtatgg gtcaacaaaa atgattgcag
     1621 agggggtgca aaacagtcaa gtcgagaaaa tatgaaaaac agaaaacaaa gaacagaaaa
     1681 atgggtttga gagtcagtat aatttataaa agaaaaattg tacatagaaa ttaaccattt
     1741 ttgtagaaga agttattttt caagcatcgt taaaaattat tcaaagcacc ttatttcata
     1801 tttaatttta aacatggtta aatgaacaac acggtgcgca atcaggaaaa cttgaaatct
     1861 gaaactgttg ttgtgatctt cttcgcaact gttcagatag cactagtgta atgttaagag
     1921 tgcgcgaata taatggaata taatggatca cacctcctgc catcaggtaa acgtctctgt
     1981 tatcacatat ttccaactat taaattttta ccttttacag ttttacattt ttttgaaaaa
     2041 agtaactttt tgtcttcaaa atccctgacg aaaatatcaa atattttaat cgagactgca
     2101 gaggaaccga ttgatgattt ggaaaatcca gctttacctg tgtaagaact gaaaagtttc
     2161 ataaccctag ggtattccca gttacattcc ccactggcta acaatagcac ccagtttttc
     2221 atcacctttc ttcaaatttc tcggcgattt gttaaaaaca aaatttgtgt cccttctctg
     2281 atatctctat gtctctaaac acaagttcat cggaaaacga aggagggtag gtgttggttg
     2341 ggctcccgaa gtgaaaatag aagagcaaga atagaatatt agagagagag tgcagagagg
     2401 gcgggatagc tcccgggatt ccgttttctt cttctttatc ttcaacgatg atgtgtgtgc
     2461 gtgttgtata gattctgttg ctcccccaca actcgctccg aaggctcaat acaattcaat
     2521 tgatattgga ggagagccta ccggagtggg aggataagaa gaaacataag aagaagaaga
     2581 agaagaagca tgcttctggt ttttgatgct atgaaaacgg cacaaaaaga tgattgaggt
     2641 cccttttcaa taccttctct catctttcaa atcccattga aacctaaaac ttctcaccac
     2701 gctttaccat tgttctccaa aaacttatag caatgtctat aactttttta tctctgaaaa
     2761 gcagtgttcc atttttcttt ttcctatttt atttcaattg tttctcacat ttcgtttgga
     2821 ttctttgctt gtcaaccagc ttcttcttcc acttttaccg tctaattttc agggcaggga
     2881 gccatcaaac ccacgaccac tagatccatc tagcccggga tgaatattgt cagatgtcgg
     2941 agacgacaca aaattttgga aaatttggaa gaagagaatc tcggcccgag ctgctcgtcg
     3001 acgacttcaa caaccgctgc caccgaagct ctcggaacaa ccactgagga tatgaggctt
     3061 aagcagcagc gaagctcgtc gcgtgccacg gagcacgata ttgtcgacgg caatcaccac
     3121 gacgacgagc acatcacaat gagacggctt cgacttgtca aaaattcgcg gacgcggcgt
     3181 agaacgacgc ccgattcaag tatggactgc tatgaggaaa acccgccatc acaaaaaact
     3241 tcaataaatt attcttggat ttctaaaaag tcatcaatga cgtcattaat gcttttactg
     3301 ctattcgctt ttgtacagcc gtgtgcctca atagtcgaaa aacgatgcgg cccaatcgat
     3361 attcgaaata ggccgtggga tattaagccg caatggtcga aacttggtga tccgaacgaa
     3421 aaagatttgg ctggtcagag aatggtcaac tgcacagtgg tggaaggttc gctgacaatc
     3481 tcatttgtac tgaaacacaa gacaaaagca caagaagaaa tgcatcgaag tctacagcca
     3541 agatattccc aagacgaatt tatcactttt ccgcatctac gtgaaattac tggaactctg
     3601 ctcgtttttg agactgaagg attagtggat ttgcgtaaaa ttttcccaaa tcttcgtgta
     3661 attggaggcc gttcgctgat tcaacactat gcgctgataa tttatcgaaa tccggatttg
     3721 gagatcggtc ttgacaagct ttccgtaatt cgaaatggtg gtgtacggat aatcgataat
     3781 cgaaaactgt gctacacgaa aacgattgat tggaaacatt tgatcacttc ttccatcaac
     3841 gatgttgtcg ttgataatgc tgccgagtac gctgtcactg agactggatt gatgtgccca
     3901 cgtggagctt gcgaagagga taaaggcgaa tcaaagtgtc attatttgga ggaaaagaat
     3961 caggaacaag gtgtcgaaag agttcagagt tgttggtcga acaccacttg ccaaaagtct
     4021 tgtgcttatg atcgtcttct tccaacgaaa gaaatcggac cgggatgtga tgcgaacggc
     4081 gatcgatgtc acgatcaatg cgtgggcggt tgtgagcgtg tgaatgatgc gacagcatgc
     4141 cacgcgtgca agaatgtcta tcacaaggga aagtgtatcg aaaagtgtga tgctcacctg
     4201 taccttctcc ttcaacgtcg ttgtgtgacc cgtgagcagt gtctgcagct gaatccggtg
     4261 ctctcgaaca aaacagtgcc tatcaaggcg acggcaggcc tttgctcgga taaatgtccc
     4321 gatggttatc aaatcaaccc ggatgatcat cgagaatgcc gaaaatgcgt tggcaagtgt
     4381 gagattgtgt gcgagatcaa tcacgtcatt gatacgtttc cgaaggcaca ggcgatcagg
     4441 ctatgcaata ttattgacgg aaatctgacg atcgagattc gcggaaaaca ggattcggga
     4501 atggcgtccg agttgaagga tatatttgcg aacattcaca cgatcaccgg ctacctgttg
     4561 gtacgtcaat cgtcaccgtt tatctcgttg aacatgttcc ggaatttacg acgtattgag
     4621 gcaaagtcac tgttcagaaa tctatatgct atcacagttt ttgaaaatcc gaatttaaaa
     4681 aagctattcg attcaacgac ggatttgacg cttgatcgtg gaactgtgtc aattgccaat
     4741 aacaagatgt tatgcttcaa gtatatcaag cagctaatgt caaagttaaa tataccactc
     4801 gatccgatag atcaatcaga agggacaaat ggtgagaagg caatctgtga ggatatggca
     4861 atcaacgtga gcatcacagc ggtcaacgcg gactcggtct tctttagttg gccctcattc
     4921 aacattaccg atatagatca gcgaaagttt ctcggctacg agctcttctt caaagaagtc
     4981 ccacgaatcg atgagaacat gacgatcgaa gaggatcgaa gtgcgtgtgt cgattcgtgg
     5041 cagagtgtct tcaaacagta ctacgagacg tcgaacggtg aaccgacccc ggacattttt
     5101 atggatattg gaccgcgcga gcgaattcgg ccgaatacgc tctacgcgta ctatgtggcg
     5161 acgcagatgg tgttgcatgc cggtgcgaag aacggtgtat cgaagattgg ttttgtgagg
     5221 acgagctact atacgcctga tcctccgacg ttggcactag cgcaagtcga ttcggacgct
     5281 attcatatta cgtgggaagc gccgctccaa ccgaacggag acctcacgca ttacacaatt
     5341 atgtggcgtg agaatgaagt gagcccgtac gaggaagccg aaaagttttg tacagatgca
     5401 agcacccccg caaatcgaca acacacgaaa gatccgaaag agacgattgt agccgataag
     5461 ccagtcgata ttccgtcatc acgtaccgta gctccgacac ttttgactat gatgggtcac
     5521 gaagatcagc agaaaacgtg cgctgcaacg cccggttgtt gttcgtgttc ggctatcgaa
     5581 gaatcatcgg aacagaacaa gaagaagcga ccggatccga tgtcggcgat cgaatcatct
     5641 gcatttgaga ataagctgtt ggatgaggtt ttaatgccga gagacacgat gcgagtgaga
     5701 cgatcaattg aagacgcgaa tcgagtcagt gaagagttgg aaaaagctga aaatttggga
     5761 aaagctccaa aaactctcgg tggaaagaag ccgctgatcc atatttcgaa gaagaagccg
     5821 tcgagcagca gcaccacatc cacaccggct ccaacgatcg catcaatgta tgccttaaca
     5881 aggaaaccga ctacggtgcc gggaacaagg attcggctct acgagatcta cgaaccttta
     5941 cccggaagct gggcgattaa tgtatcagct ctggcattgg ataatagtta tgtgatacga
     6001 aatttgaagc attacacact ttatgcgatt tctctatccg cgtgccaaaa catgacagta
     6061 cccggagcat cttgctcaat atcccatcgt gcgggagcat tgaaacgaac aaaacacatc
     6121 acagacattg ataaagtgtt gaatgaaaca attgaatgga gatttatgaa taatagtcaa
     6181 caagtcaacg tgacgtggga tccaccgact gaagtgaatg gtggaatatt cggttatgtt
     6241 gtaaagctta agtcaaaagt cgatggatca attgttatga cgagatgtgt cggtgcgaag
     6301 agaggatatt caacacggaa tcagggtgtc ctattccaga atttggccga tggacgttat
     6361 tttgtctcag taacggcgac ctctgtacac ggcgctggac cggaagccga atcctccgac
     6421 ccaatcgtcg tcatgacgcc aggcttcttc actgtggaaa tcattctcgg catgcttctc
     6481 gtctttttga ttttaatgtc aattgccggt tgtataatct actactacat tcaagtacgc
     6541 tacggcaaaa aagtgaaagc tctatctgac tttatgcaat tgaatcccga atattgtgtg
     6601 gacaataagt acaatgcaga cgattgggag ctacggcagg atgatgttgt gctcggacaa
     6661 cagtgtggag agggatcatt cggaaaagtg tacctcggaa ctggaaataa tgttgtttct
     6721 ctgatgggtg atcgtttcgg accgtgtgct attaagatta atgtagatga tccagcgtcg
     6781 actgagaatc tcaactatct catggaagct aatattatga agaactttaa gactaacttt
     6841 atcgtcaaac tgtacggagt tatctctact gtacaaccag cgatggttgt gatggaaatg
     6901 atggatcttg gaaatctccg tgactatctc cgatcgaaac gcgaagacga agtgttcaat
     6961 gagacggact gcaacttttt cgacataatc ccgagggata aattccatga gtgggccgca
     7021 cagatttgtg atggtatggc gtacctggag tcgctcaagt tttgccatcg agatctcgcc
     7081 gcacgtaatt gcatgataaa tcgggatgag actgtcaaga ttggagattt cggaatggct
     7141 cgtgatctat tctatcatga ctattataag ccatcgggca agcgtatgat gcctgttcga
     7201 tggatgtcac ccgagtcgtt gaaagacgga aagtttgact cgaaatctga tgtttggagc
     7261 ttcggagttg ttctctatga aatggttaca ctcggtgctc agccatatat tggtttgagt
     7321 aatgatgagg tgttgaatta tattggaatg gcccggaagg ttatcaagaa gcccgaatgt
     7381 tgtgaaaact attggtataa ggtgatgaaa atgtgctgga gatactcacc tcgggatcgt
     7441 ccgacgttcc tccagctcgt tcatcttcta gcagctgaag cttcaccaga attccgagat
     7501 ttatcatttg tcctaaccga taatcaaatg atccttgacg attcagaagc actggatctt
     7561 gatgatattg atgatactga tatgaatgat caggttgtcg aggtggcacc ggatgttgag
     7621 aacgtcgagg ttcagagtga ttcggaacgt cggaatacgg attcaatacc gttgaaacag
     7681 tttaagacga tccctccgat caatgcgacg acgagtcatt cgacaatatc gattgatgag
     7741 acaccgatga aagcgaagca gcgagaagga tcgctggatg aggagtacgc attgatgaat
     7801 catagtggag gtccgagtga tgcggaagtt cggacgtatg ctggtgatgg agattatgtg
     7861 gagagagatg ttcgagagaa tgatgtgcca acgcgacgaa atactggtgc atcaacatca
     7921 agttacacag gtggtggtcc atattgccta acaaatcgtg gtggttcaaa tgaacgagga
     7981 gccggtttcg gtgaagcagt acgattaact gatggtgttg gaagtggaca tttaaatgat
     8041 gatgattatg ttgaaaaaga gatatcatcc atggatacgc gccggagcac gggcgcctcg
     8101 agctcttcct acggtgttcc acagacgaat tggagtggaa atcgtggtgc cacgtattat
     8161 acgagtaaag ctcaacaggc agcaactgca gcagcagcag cagcagcagc tctccaacag
     8221 caacaaaatg gtggtcgagg cgatcgatta actcaactac ccggaactgg acatttacaa
     8281 tcgacacgtg gtggacaaga tggagattat attgaaactg aaccgaaaaa ttatagaaat
     8341 aatggatctc catcggtact ggattttttt ttttggagaa attagccgga aaattcgatt
     8401 tttttcgctg aaaatctgaa aaaatttggc taaaaattgc cctgaaacac gtttttttcg
     8461 gaattttcat ataaaaatgg acattttcgt aaaaaattgg ctatgtgttg tgaatttttt
     8521 ctgactttta aaggcctttg aactaggaat attaaaattt tattgaaaat tattgaaaag
     8581 tgcaaatttt tatttttttt aggtgaaaaa agcttagatt taggcgaatt ttctgaaatt
     8641 ttatagtgaa tttggttctc aggatattag ccattttttc ccgatttttt ggtaaaaatt
     8701 tgttaaaacc cgcttcaaat gactgtagtt ttatacaaaa ataactaaaa attcgatttt
     8761 tcaattattc agctcgaaaa agcccaacaa aaatatttaa aatttgaaga aaaaagctaa
     8821 aaatgagtaa aaatgactgg tttttttcgc tttttctatt tgaataaatt tctgaaaaat
     8881 gacgaaaatc tgaaaaaatt accccggaca ttgggaaatt tcgaaaaaat cttgaaaata
     8941 gtctgaaatt tttaaaattt acagattttc acaaaaaagg gaaatttcgc atttcctgaa
     9001 attgcccaaa aatttttttt tcattttttt aaaaattctg gaaatttatt tttggctgtt
     9061 tcaaattttt aaaaaaagaa aaatcccaaa aattggtgaa aactccatta aaattctcca
     9121 gaaaattcaa attttccatg aaaaattgtt ttaaaagcat gaaaatctgc cgatttttgt
     9181 ctaaaatttt taaaaatatt tcaaaaattt cagaaaaaac aggaaaagcg aatattacat
     9241 aaataaaaaa tctggaaatt ctagaaactg aaaatttttt aatttttccg ctcaaaaatt
     9301 ctaatattcg atgaaaatga gcccgaaaat tcgagtaaat ttccaaaaaa aatttttttt
     9361 tttttcaaac acaaatatta atttttatta atttttcagc gaaacggcaa cagccgtgac
     9421 attttcaacg gacgttcggc tttcggtgaa aatgagcatc taatcgagga taatgagcat
     9481 ccacttgtgg taccatggta ttgatatctg agctccgcat cggccgctgt catcagatcg
     9541 ccatctcgcg cccgtgcctc tgacttctaa gtccaattac tcttcaacat ccctacatgc
     9601 tctttctccc tgtgctccca ccccctattt ttgttattat caaaaaactt ctcttaattt
     9661 ctttgttttt tagcttcttt taagtcacct ctaacaatga aattgtgtag attcaaaaat
     9721 agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca ttaataataa
     9781 ttctatccca aaatctacac aatgttctgt gtacacttct tatgtttttt acttctgata
     9841 aatttttttg aaacatcata gaaaaaaccg cacacaaaat accttatcat atgttacgtt
     9901 tcagtttatg accgcaattt ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc
     9961 atgctcatcg tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag tgaaagttta
    10021 tgaaattaat tttcctgctt ttgctttttg gggtttcccc tattgtttgt caagatttcg
    10081 aggacggcgt ttttcttgct aaaatcacaa gtattgatga gcacgatgca agaaagatcg
    10141 gaagaaggtt tgggtttgag gctcagtgga aggtgagtag aagttgataa tttgaaagtg
    10201 gagtagtgtc tatggggttt ttgccttaaa tgacagaata cattcccaat ataccaaaca
    10261 taactgtttc tactagtggc cacgtgggcc gtgcacctta agcttggcac tggccgtcgt
    10321 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
    10381 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
    10441 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
    10501 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
    10561 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
    10621 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
    10681 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
    10741 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
    10801 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
    10861 acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc
    10921 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
    10981 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
    11041 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
    11101 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
    11161 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
    11221 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
    11281 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
    11341 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
    11401 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
    11461 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
    11521 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
    11581 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
    11641 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
    11701 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
    11761 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    11821 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    11881 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
    11941 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    12001 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    12061 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
    12121 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    12181 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    12241 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    12301 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    12361 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    12421 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    12481 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    12541 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    12601 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    12661 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    12721 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    12781 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    12841 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    12901 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    12961 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    13021 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    13081 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    13141 acgaccgagc gcagcgagtc agtgagcgag gaa
//
