LOCUS Exported 1494 bp ds-DNA linear SYN 20-APR-2022 DEFINITION Soluble light-dependent effector protein (HP1alpha with I163A mutation) for optodroplet formation or recruitment to CIBN localizers, . ACCESSION . VERSION . KEYWORDS pPHR-mCherry-mHP1alpha-I163A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1494) TITLE Rippe Lab Plasmids 2022 REFERENCE 2 (bases 1 to 1494) AUTHORS . TITLE Direct Submission JOURNAL Exported Apr 20, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1494 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1..1494 /codon_start=1 /product="N-terminal photolyase homology region of Arabidopsis thaliana cryptochrome 2" /label=CRY2PHR /note="binds to CIB1 upon exposure to blue light (Kennedy et al., 2010)" /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA" ORIGIN 1 atgaagatgg acaaaaagac tatagtttgg tttagaagag acctaaggat tgaggataat 61 cctgcattag cagcagctgc tcacgaagga tctgtttttc ctgtcttcat ttggtgtcct 121 gaagaagaag gacagtttta tcctggaaga gcttcaagat ggtggatgaa acaatcactt 181 gctcacttat ctcaatcctt gaaggctctt ggatctgacc tcactttaat caaaacccac 241 aacacgattt cagcgatctt ggattgtatc cgcgttaccg gtgctacaaa agtcgtcttt 301 aaccacctct atgatcctgt ttcgttagtt cgggaccata ccgtaaagga gaagctggtg 361 gaacgtggga tctctgtgca aagctacaat ggagatctat tgtatgaacc gtgggagata 421 tactgcgaaa agggcaaacc ttttacgagt ttcaattctt actggaagaa atgcttagat 481 atgtcgattg aatccgttat gcttcctcct ccttggcggt tgatgccaat aactgcagcg 541 gctgaagcga tttgggcgtg ttcgattgaa gaactagggc tggagaatga ggccgagaaa 601 ccgagcaatg cgttgttaac tagagcttgg tctccaggat ggagcaatgc tgataagtta 661 ctaaatgagt tcatcgagaa gcagttgata gattatgcaa agaacagcaa gaaagttgtt 721 gggaattcta cttcactact ttctccgtat ctccatttcg gggaaataag cgtcagacac 781 gttttccagt gtgcccggat gaaacaaatt atatgggcaa gagataagaa cagtgaagga 841 gaagaaagtg cagatctttt tcttagggga atcggtttaa gagagtattc tcggtatata 901 tgtttcaact tcccgtttac tcacgagcaa tcgttgttga gtcatcttcg gtttttccct 961 tgggatgctg atgttgataa gttcaaggcc tggagacaag gcaggaccgg ttatccgttg 1021 gtggatgccg gaatgagaga gctttgggct accggatgga tgcataacag aataagagtg 1081 attgtttcaa gctttgctgt gaagtttctt ctccttccat ggaaatgggg aatgaagtat 1141 ttctgggata cacttttgga tgctgatttg gaatgtgaca tccttggctg gcagtatatc 1201 tctgggagta tccccgatgg ccacgagctt gatcgcttgg acaatcccgc gttacaaggc 1261 gccaaatatg acccagaagg tgagtacata aggcaatggc ttcccgagct tgcgagattg 1321 ccaactgaat ggatccatca tccatgggac gctcctttaa ccgtactcaa agcttctggt 1381 gtggaactcg gaacaaacta tgcgaaaccc attgtagaca tcgacacagc tcgtgagcta 1441 ctagctaaag ctatttcaag aacccgtgaa gcacagatca tgatcggagc agca //