LOCUS       Exported               10334 bp ds-DNA     circular SYN 13-JAN-2022
DEFINITION  A C-terminal APEX2 fusion to the human ATGL S47A protein.
ACCESSION   .
VERSION     .
KEYWORDS    ATGL*-V5-APEX2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10334)
  AUTHORS   Bersuker K, Peterson CWH, To M, Sahl SJ, Savikhin V, Grossman EA, 
            Nomura DK, Olzmann JA
  TITLE     A Proximity Labeling Strategy Provides Insights into the Composition
            and Dynamics of Lipid Droplet Proteomes.
  JOURNAL   Dev Cell. 2018 Jan 8;44(1):97-112.e7. doi: 
            10.1016/j.devcel.2017.11.020. Epub 2017 Dec 21.
  PUBMED    29275994
REFERENCE   2  (bases 1 to 10334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 13, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10334
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1941..2244
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2245..2448
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2399..2419
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2450..2468
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2471..2489
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2530..2554
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4130..4171
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4172..4918
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     protein_bind    complement(4939..4963)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4994..5582
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5047..5067)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5465..5476)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5596..6095
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5631..5654)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6028..6047
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6116..6715
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6116..6135)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6612..6632
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6853..7086
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7158..7279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7195..7214)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7249..7268
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7319..7454
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7381..7400
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(7474..7493)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7475..7493)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7503..7520)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7503..7519)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7512..7534)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7661..8116
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7748..7767)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7958..7979
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8142..8246
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8247..9107
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8465..8484)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9278..9866
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9767..9786
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10020..10037
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10154..10175
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10190..10220
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10228..10244
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10233..10255
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10252..10268
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10252..10268
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10287..10307
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10289..10307
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat
     1921 cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     1981 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2041 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     2101 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2161 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     2221 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2281 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2341 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2401 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt
     2461 gatagagatc tccctatcag tgatagagat cgtcgactag tccagtgtgg tggaattctg
     2521 cagatatcaa caagtttgta caaaaaagca ggctttaaag gaaccaattc agtcgactgg
     2581 atccggtacc gaattcgcgg ccgccaccat gtttccccgc gagaagacgt ggaacatctc
     2641 gttcgcgggc tgcggcttcc tcggcgtcta ctacgtcggc gtggcctcct gcctccgcga
     2701 gcacgcgccc ttcctggtgg ccaacgccac gcacatctac ggcgccgcgg ccggggcgct
     2761 cacggccacg gcgctggtca ccggggtctg cctgggtgag gctggtgcca agttcattga
     2821 ggtatctaaa gaggcccgga agcggttcct gggccccctg cacccctcct tcaacctggt
     2881 aaagatcatc cgcagtttcc tgctgaaggt cctgcctgct gatagccatg agcatgccag
     2941 tgggcgcctg ggcatctccc tgacccgcgt gtcagacggc gagaatgtca ttatatccca
     3001 cttcaactcc aaggacgagc tcatccaggc caatgtctgc agcggtttca tccccgtgta
     3061 ctgtgggctc atccctccct ccctccaggg ggtgcgctac gtggatggtg gcatttcaga
     3121 caacctgcca ctctatgagc ttaagaacac catcacagtg tcccccttct cgggcgagag
     3181 tgacatctgt ccgcaggaca gctccaccaa catccacgag ctgcgggtca ccaacaccag
     3241 catccagttc aacctgcgca acctctaccg cctctccaag gccctcttcc cgccggagcc
     3301 cctggtgctg cgagagatgt gcaagcaggg ataccgggat ggcctgcgct ttctgcagcg
     3361 gaacggcctc ctgaaccggc ccaacccctt gctggcgttg ccccccgccc gcccccacgg
     3421 cccagaggac aaggaccagg cagtggagag cgcccaagcg gaggattact cgcagctgcc
     3481 gggagaagat cacatcctgg agcacctgcc cgcccggctc aatgaggccc tgctggaggc
     3541 ctgcgtggag cccacggacc tgctgaccac cctctccaac atgctgcctg tgcgtctggc
     3601 cacggccatg atggtgccct acacgctgcc actggagagc gctctgtcct tcaccatccg
     3661 cttgctggag tggctgcccg acgttcccga ggacatccgg tggatgaagg agcagacggg
     3721 cagcatctgc cagtacctgg tgatgcgcgc caagaggaag ctgggcaggc acctgccctc
     3781 caggctgccg gagcaggtgg agctgcgccg cgtccagtcg ctgccgtccg tgccgctgtc
     3841 ctgcgccgcc tacagagagg cactgcccgg ctggatgcgc aacaacctct cgctggggga
     3901 cgcgctggcc aagtgggagg agtgccagcg ccagctgctg ctcggcctct tctgcaccaa
     3961 cgtggccttc ccgcccgaag ctctgcgcat gcgcgcaccc gccgacccgg ctcccgcccc
     4021 cgcggaccca gcatccccgc agcaccagct ggccgggcct gcccccttgc tgagcacccc
     4081 tgctcccgag gcccggcccg tgatcggggc cctggggctg aaggatccag gcaagcccat
     4141 ccccaacccc ctgctgggcc tggacagcac cggaaagtct tacccaactg tgagtgctga
     4201 ttaccaggac gccgttgaga aggcgaagaa gaagctcaga ggcttcatcg ctgagaagag
     4261 atgcgctcct ctaatgctcc gtttggcatt ccactctgct ggaacctttg acaagggcac
     4321 gaagaccggt ggacccttcg gaaccatcaa gcaccctgcc gaactggctc acagcgctaa
     4381 caacggtctt gacatcgctg ttaggctttt ggagccactc aaggcggagt tccctatttt
     4441 gagctacgcc gatttctacc agttggctgg cgttgttgcc gttgaggtca cgggtggacc
     4501 taaggttcca ttccaccctg gaagagagga caagcctgag ccaccaccag agggtcgctt
     4561 gcccgatccc actaagggtt ctgaccattt gagagatgtg tttggcaaag ctatggggct
     4621 tactgaccaa gatatcgttg ctctatctgg gggtcacact attggagctg cacacaagga
     4681 gcgttctgga tttgagggtc cctggacctc taatcctctt attttcgaca actcatactt
     4741 cacggagttg ttgagtggtg agaaggaagg tctccttcag ctaccttctg acaaggctct
     4801 tttgtctgac cctgtattcc gccctctcgt tgataaatat gcagcggacg aagatgcctt
     4861 ctttgctgat tacgctgagg ctcaccaaaa gctttccgag cttgggtttg ctgatgccta
     4921 atgactcgag atatctagac ccagctttct tgtacaaagt ggttgatatc cagcacagtg
     4981 gcggccgctc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     5041 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     5101 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     5161 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     5221 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     5281 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     5341 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     5401 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     5461 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     5521 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     5581 gcctggaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     5641 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     5701 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     5761 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     5821 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     5881 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     5941 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     6001 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     6061 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac
     6121 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     6181 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     6241 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     6301 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     6361 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     6421 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     6481 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     6541 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     6601 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     6661 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc
     6721 gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat ctcgagggcc
     6781 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     6841 aaaagggggg actggaaggg ctagctcact cccaacgaag acaagatctg ctttttgctt
     6901 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6961 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7021 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7081 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     7141 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7201 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7261 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     7321 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7381 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     7441 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     7501 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     7561 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     7621 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     7681 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     7741 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     7801 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     7861 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     7921 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     7981 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     8041 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     8101 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     8161 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     8221 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     8281 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     8341 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     8401 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     8461 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     8521 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     8581 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     8641 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     8701 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     8761 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     8821 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     8881 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     8941 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     9001 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     9061 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     9121 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     9181 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9241 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     9301 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     9361 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     9421 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     9481 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     9541 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     9601 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     9661 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     9721 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     9781 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     9841 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     9901 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     9961 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    10021 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    10081 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    10141 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    10201 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    10261 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
    10321 agctgcaagc ttaa
//
