LOCUS       Exported                3711 bp ds-DNA     circular SYN 14-JAN-2022
DEFINITION  Sars-CoV-2 protein mutation.
ACCESSION   .
VERSION     .
KEYWORDS    pDON223 NSP5 K90R
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, 
            Pohner I, Ohman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S,
            Vartiainen MK, Pietiainen V, Poso A, Varjosalo M
  TITLE     SARS-CoV-2-host proteome interactions for antiviral drug discovery.
  JOURNAL   Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396.
  PUBMED    34709727
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 14, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      55..82
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      174..260
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     309..331
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     323..340
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     324..340
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    356..455
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1377..1476)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1493..1512)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1494..1512)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1517..1533)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1517..1533)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1964..2755
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2848..3436
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3337..3356
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3590..3607
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg
       61 ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct
      121 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc
      181 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac
      241 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg
      301 gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccccaaat
      361 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt
      421 ttttataatg ccaactttgt acaaaaaagt tggcatgtcc ggattccgca agatggcctt
      481 cccttctggc aaggtggagg gctgcatggt gcaggtgacc tgcggaacca ccaccctgaa
      541 cggtctgtgg ctggacgacg tggtgtactg ccccaggcac gtgatctgca cctctgagga
      601 catgctgaac cctaactacg aagacctgct gatcagaaag agcaaccaca acttcctggt
      661 gcaggccggt aacgtgcagc tgcgtgtgat cggccactct atgcagaact gcgtgctgaa
      721 gctgagggtg gacaccgcta accccaagac ccctaagtac aagttcgtga gaatccagcc
      781 tggtcagacc ttctccgtgc tggcctgcta caacggaagc ccatccggcg tgtaccagtg
      841 cgctatgcgt cccaacttca ccatcaaggg cagcttcctg aacggctctt gcggaagcgt
      901 gggtttcaac atcgactacg actgcgtgtc cttctgctac atgcaccaca tggagctgcc
      961 aactggagtg cacgctggaa ctgacctgga gggtaacttc tacggcccct tcgtggacag
     1021 acagactgct caggctgctg gcaccgacac caccatcacc gtgaacgtgc tggcttggct
     1081 gtacgccgct gtgatcaacg gagaccgctg gttcctgaac aggttcacca ccaccctgaa
     1141 cgacttcaac ctggtggcta tgaagtacaa ctacgagcct ctgacccagg accacgtgga
     1201 tatcctgggt ccactgagcg cccagactgg aatcgctgtg ctggacatgt gcgcctccct
     1261 gaaggaactg ctgcagaacg gcatgaacgg acgcaccatc ctgggatccg ctctgctgga
     1321 ggacgaattc acccccttcg acgtggtgag gcagtgctct ggtgtgacct tccagtaccc
     1381 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     1441 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     1501 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     1561 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga
     1621 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg
     1681 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     1741 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     1801 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     1861 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     1921 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat
     1981 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     2041 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     2101 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     2161 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     2221 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     2281 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     2341 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     2401 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     2461 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     2521 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     2581 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     2641 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     2701 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc
     2761 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     2821 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2881 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2941 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3001 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3061 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3121 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3181 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3241 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3301 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3361 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3421 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3481 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     3541 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     3601 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     3661 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg a
//
