LOCUS Exported 11113 bp ds-DNA circular SYN 19-JAN-2022 DEFINITION 2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmid. ACCESSION . VERSION . KEYWORDS pLVTHM SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11113) AUTHORS Wiznerowicz M, Trono D TITLE Conditional suppression of cellular genes: lentivirus vector-mediated drug-inducible RNA interference. JOURNAL J Virol. 2003 Aug . 77(16):8957-61. PUBMED 12885912 REFERENCE 2 (bases 1 to 11113) AUTHORS . TITLE Direct Submission JOURNAL Exported Jan 19, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11113 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 431..1019 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS 537..548 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind 946..966 /label=WPRE-R /note="WPRE, reverse primer" CDS complement(1094..1810) /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS complement(1094..1810) /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1136..1157) /label=EGFP-C /note="EGFP, forward primer" primer_bind 1485..1504 /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1744..1765 /label=EGFP-N /note="EGFP, reverse primer" regulatory 1807..1816 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" misc_feature 1901..2018 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter complement(2066..3244) /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2075..3013 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind complement(2098..2118) /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" CDS complement(3629..3673) /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 3858..4091 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 4584..4709 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" LTR 4756..5389 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" polyA_signal 5557..5691 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5581..5600) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 5635..5654 /label=SV40pA-R /note="SV40 polyA, reverse primer" CDS complement(6116..6136) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" intron 6266..6331 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" promoter complement(7465..7794) /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7479..7614 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(7533..7552) /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind 7779..7799 /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" primer_bind complement(7869..7886) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8040..8628) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8120..8139) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8799..9659) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9422..9441 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9660..9764) /gene="bla" /label=AmpR promoter primer_bind 9832..9850 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(9888..9910) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 10010..10029 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 10243..10262 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" promoter 10552..10570 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 10552..10569 /label=SP6 /note="SP6 promoter, forward primer" LTR 10595..10775 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" protein_bind complement(10795..10828) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." promoter complement(10863..11077) /label=H1 promoter /note="human H1 RNA promoter" primer_bind complement(10923..10942) /label=H1 /note="Human H1 promoter, forward primer" protein_bind 11106..263 /label=tetracycline response element /bound_moiety="tetracycline repressor TetR" /note="contains seven copies of the tetracycline operator tetO" ORIGIN 1 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa 61 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc 121 tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag 181 tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac tcgactttca 241 cttttctcta tcactgatag ggagtggtaa actcgagggg gatccactag catggtgaat 301 tgatcccatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc ttttctttta 361 aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaaggt acctggccat 421 acgtaccgat gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga 481 agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg 541 agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg 601 agcagccatg gaaaggacgt cagcttcccc gacaacacca cggaattgtc agtgcccaac 661 agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata 721 gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca 781 gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac 841 aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata 901 cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga 961 gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgatta 1021 tcatatgact agtccccgaa gcttgagctc gactagctag ctagctacta gctagtcgag 1081 atctgagtcc ggacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga 1141 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg 1201 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct 1261 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca 1321 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt 1381 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga 1441 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt 1501 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga 1561 agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg 1621 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg 1681 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt 1741 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct 1801 tgctcaccat ggtggcgacc ggtagcgcta ggacgcgcgc gtaagcttag gccttcgaag 1861 atcccgtagt ttaaacctcg aggctagtct cgtgatcgat aaaattttga atttttgtaa 1921 tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc 1981 ctgcactgta ccccccaatc cccccttttc ttttaaaagt taaccgatac cgtcgagatt 2041 aattaaattt aaatgtcgaa attcctcacg acacctgaaa tggaagaaaa aaactttgaa 2101 ccactgtctg aggcttgaga atgaaccaag atccaaactc aaaaagggca aattccaagg 2161 agaattacat caagtgccaa gctggcctaa cttcagtctc cacccactca gtgtggggaa 2221 actccatcgc ataaaacccc tccccccaac ctaaagacga cgtactccaa aagctcgaga 2281 actaatcgag gtgcctggac ggcgcccggt actccgtgga gtcacatgaa gcgacggctg 2341 aggacggaaa ggcccttttc ctttgtgtgg gtgactcacc cgcccgctct cccgagcgcc 2401 gcgtcctcca ttttgagctc cctgcagcag ggccgggaag cggccatctt tccgctcacg 2461 caactggtgc cgaccgggcc agccttgccg cccagggcgg ggcgatacac ggcggcgcga 2521 ggccaggcac cagagcaggc cggccagctt gagactaccc ccgtccgatt ctcggtggcc 2581 gcgctcgcag gccccgcctc gccgaacatg tgcgctggga cgcacgggcc ccgtcgccgc 2641 ccgcggcccc aaaaaccgaa ataccagtgt gcagatcttg gcccgcattt acaagactat 2701 cttgccagaa aaaaagcgtc gcagcaggtc atcaaaaatt ttaaatggct agagacttat 2761 cgaaagcagc gagacaggcg cgaaggtgcc accagattcg cacgcggcgg ccccagcgcc 2821 caggccaggc ctcaactcaa gcacgaggcg aaggggctcc ttaagcgcaa ggcctcgaac 2881 tctcccaccc acttccaacc cgaagctcgg gatcaagaat cacgtactgc agccaggtgg 2941 aagtaattca aggcacgcaa gggccataac ccgtaaagag gccaggcccg cgggaaccac 3001 acacggcact tacctgtgtt ctggcggcaa acccgttgcg aaaaagaacg ttcacggcga 3061 ctactgcact tatatacggt tctcccccac cctcgggaaa aaggcggagc cagtacacga 3121 catcactttc ccagtttacc ccgcgccacc ttctctaggc accggttcaa ttgccgaccc 3181 ctccccccaa cttctcgggg actgtgggcg atgtgcgctc tgcccactga cgggcaccgg 3241 agccaattcc cactcctttc aagacctaga aggtccatta gctgcaaaga ttcctctctg 3301 tttaaaactt tatccatctt tgcaaagctt atcgtcgaca tcgcgatggt cgaggctagt 3361 ctcgtgatcg ataccgtcga gatccgttca ctaatcgaat ggatctgtct ctgtctctct 3421 ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg 3481 tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa 3541 aaactattct taaacctacc aagcctccta ctatcattat gaataatttt atataccaca 3601 gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt 3661 cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg 3721 tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca 3781 gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag 3841 agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc 3901 ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa 3961 tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta 4021 ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga 4081 acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg 4141 tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc 4201 aattgtccct catatctcct cctccaggtc tgaagatcag cggccgcttg ctgtgcggtg 4261 gtcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt gtcttttatc 4321 tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga tctaagttct 4381 tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca gccttctgat 4441 gtttctaaca ggccaggatt aactgcgaat cgttctagct ccctgcttgc ccatactata 4501 tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc ccatcgcgat 4561 ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt ctagcctccg 4621 ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct 4681 tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc tttcaagtcc 4741 ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg agggatctct 4801 agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt 4861 attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt 4921 ctaaccagag agacccagta caggcaaaaa gcagctgctt atatgcagga tctgagggct 4981 cgccactccc cagtcccgcc caggccacgc ctccctggaa agtccccagc ggaaagtccc 5041 ttgtagcaag ctcgatatca gcagttcttg aagtactccg gatgcagctc tcgggccacg 5101 tgatgaaatg ctaggcggct gtcaaacctc cactctaaca cttctctctc cgggtcatcc 5161 atcccatgca ggctcacagg gtgtaacaag ctggtgttct ctcctttatt ggcctcttct 5221 accttatctg gctcaactgg tactagcttg tagcaccatc caaaggtcag tggatatctg 5281 acccctggcc ctggtgtgta gttctgctaa tcagggaagt agccttgtgt gtggtagatc 5341 cacagatcaa ggatatcttg tcttctttgg gagtgaatta gcccttccaa ctactaagtt 5401 tgtagtacat atttaacaaa tacaatttct ttaaaatgaa aataattcag aggaatcaca 5461 ggtttagagt aaatgaaacc acaggtaatt ggcagtggta atagggtatg gggtgggaag 5521 tttgggatga ttttggttag cttgagttat ccagttgatc cagacatgat aagatacatt 5581 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt 5641 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac 5701 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag 5761 taaaacctct acaaatgtgg tatggctgat tatgatctct agtcaaggca ctatacatca 5821 aatattcctt attaacccct ttacaaatta aaaagctaaa ggtacacaat ttttgagcat 5881 agttattaat agcagacact ctatgcctgt gtggagtaag aaaaaacagt atgttatgat 5941 tataactgtt atgcctactt ataaaggtta cagaatattt ttccataatt ttcttgtata 6001 gcagtgcagc tttttccttt gtggtgtaaa tagcaaagca agcaagagtt ctattactaa 6061 acacagcatg actcaaaaaa cttagcaatt ctgaaggaaa gtccttgggg tcttctacct 6121 ttctcttctt ttttggagga gtagaatgtt gagagtcagc agtagcctca tcatcactag 6181 atggcatttc ttctgagcaa aacaggtttt cctcattaaa ggcattccac cactgctccc 6241 attcatcagt tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa 6301 cacattatac acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt 6361 tgtccaatta tgtcacacca cagaagtaag gttccttcac aaagatccgg ggcccactca 6421 taaatccagt tgccgccacg gtagccaatc accgtatcgt ataaatcatc gtcggtacgt 6481 tcggcatcgc tcatcacaat acgtgcctgg acgtcgagga tttcgcgtgg gtcaatgccg 6541 cgccagatcc acatcagacg gttaatcatg cgataccagt gagggatggt tttaccatca 6601 agggccgact gcacaggcgg ttgtgcgccg tgattaaagc ggcggactag cgtcgaggtt 6661 tcaggatgtt taaagcgggg tttgaacagg gtttcgctca ggtttgcctg tgtcatggat 6721 gcagcctcca gaatacttac tggaaactat tgtaacccgc ctgaagttaa aaagaacaac 6781 gcccggcagt gccaggcgtt gaaaagatta gcgaccggag attggcggga cgaatacgac 6841 gcccatatcc cacggctgtt caatccaggt atcttgcggg atatcaacaa catagtcatc 6901 aaccagcgga cgaccagccg gttttgcgaa gatggtgaca aagtgcgctt ttggatacat 6961 ttcacgaatc gcaaccgcag taccaccggt atccaccagg tcatcaataa cgatgaagcc 7021 ttcgccatcg ccttctgcgc gtttcagcac tttaagctcg cgctggttgt cgtgatcgta 7081 gctggaaata caaacggtat cgacatgacg aatacccagt tcacgcgcca gtaacgcacc 7141 cggtacagac cgccacggct tacggcaata atgcctttcc attgttcaga aggcatcagt 7201 cggcttgcga gtttacgtgc atggatctgc aacatgtccc aggtgacgat gtatttttcg 7261 ctcatgtgaa gtgtcccagc ctgtttatct acggcttaaa aagtgttcga ggggaaaata 7321 ggttgcgcga gattatagag atctggcgca ctaaaaacca gtatttcaca tgagtccgcg 7381 tctttttacg cactgcctct ccctgacgcg ggataaagtg gtattctcaa acatatctcg 7441 caagcctgtc ttgtgtccaa gctttttgca aaagcctagg cctccaaaaa agcctcctca 7501 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat 7561 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg 7621 agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc 7681 tgctggggag cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg 7741 catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca 7801 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 7861 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 7921 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 7981 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 8041 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 8101 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 8161 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 8221 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 8281 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 8341 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 8401 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 8461 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 8521 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 8581 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 8641 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 8701 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 8761 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 8821 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 8881 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 8941 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 9001 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 9061 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 9121 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 9181 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 9241 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 9301 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 9361 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 9421 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 9481 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 9541 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 9601 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 9661 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 9721 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 9781 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt 9841 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 9901 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 9961 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag 10021 cagattgtac tgagagtgca ccattcgacg ctctccctta tgcgactcct gcattaggaa 10081 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa 10141 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca 10201 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata 10261 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag 10321 gatctggcta gcgatgaccc tgctgattgg ttcgctgacc atttccgggt gcgggacggc 10381 gttaccagaa actcagaagg ttcgtccaac caaaccgact ctgacggcag tttacgagag 10441 agatgatagg gtctgcttca gtaagccaga tgctacacaa ttaggcttgt acatattgtc 10501 gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg 10561 acactataga atacacggaa ttaattctag atgctgctag agattttcca cactgactaa 10621 aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga 10681 agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc 10741 tcccaggctc agatctggtc taaccagaga gacccagtac aagcaaaaag cagcataact 10801 tcgtataatg tatgctatac gaagttattc ccatgcgacg gtatcgataa gcttggagac 10861 gcgtggtctc atacagaact tataagattc ccaaatccaa agacatttca cgtttatggt 10921 gatttcccag aacacatagc gacatgcaaa tattgcaggg cgccactccc ctgtccctca 10981 cagccatctt cctgccaggg cgcacgcgcg ctgggtgttc ccgcctagtg acactgggcc 11041 cgcgattcct tggagcgggt tgatgacgtc agcgttcgaa ttcctgcagc cccgactttc 11101 acttttctct atc //