LOCUS Exported 4723 bp ds-DNA circular SYN 19-JAN-2022 DEFINITION Localization: N1 Cloning Vector, Excitation: 587, Emission: 610. ACCESSION . VERSION . KEYWORDS mCherry2-N1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4723) TITLE Michael Davidson Empty Backbones REFERENCE 2 (bases 1 to 4723) AUTHORS . TITLE Direct Submission JOURNAL Exported Jan 19, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4723 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 181..201 /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter complement(226..330) /gene="bla" /label=AmpR promoter rep_origin 357..812 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(444..463) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 654..675 /label=F1ori-F /note="F1 origin, forward primer" polyA_signal 819..940 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(830..849) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 884..903 /label=SV40pA-R /note="SV40 polyA, reverse primer" CDS complement(1062..1772) /codon_start=1 /product="derivative of the monomeric red fluorescent protein mCherry" /label=mCherry2 /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind 1609..1627 /label=mCherry-R /note="mCherry, reverse primer" regulatory 1769..1778 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" misc_feature 1780..1860 /label=MCS /note="multiple cloning site" promoter complement(1883..2086) /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind complement(1912..1932) /label=CMV-F /note="Human CMV immediate early promoter, forward primer" enhancer 2087..2390 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" rep_origin complement(2565..3153) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2645..2664) /label=pBR322ori-F /note="pBR322 origin, forward primer" polyA_signal 3482..3529 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" primer_bind 3554..3573 /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" CDS complement(3761..4555) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3872..3891) /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind 4482..4501 /label=Neo-R /note="Neomycin resistance gene, reverse primer" promoter complement(4590..224) /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4604..16 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(4658..4677) /label=SV40pro-F /note="SV40 promoter/origin, forward primer" ORIGIN 1 gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat 61 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg 121 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac 181 accctaactg acacacattc cacagctggt tctttccgcc tcaggactct tcctttttca 241 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 301 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc 361 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 421 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 481 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 541 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 601 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 661 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 721 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 781 gaattttaac aaaatattaa cgcttacaat ttacgcctta agatacattg atgagtttgg 841 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat 901 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca 961 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta 1021 caaatgtggt atggctgatt atgatctaga gtcgcggccg cttacttgta cagctcgtcc 1081 atgccgccgg tggagtggcg gccctcggcg cgttcgtact gttccacgat ggtgtagtcc 1141 tcgttgtggg aaaggatgtc caacttgatg tcgacgttgt aggcgccggg cagctgcacg 1201 ggcttcttgg ccttgtaggt ggtcttgacc tcagcgtcgt agtggccgcc gtccttcagc 1261 ttcagcctct gcttgatctc gcccttcagg gcgccgtcct cggggtacat ccgctcagtg 1321 gaggcctccc agcccatggt acgacactgc attacggggc cgtcggaggg gaagttggtg 1381 ccgcgcagct tcaccttgta gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg 1441 gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc aattgaagcc ctcggggaag 1501 gacagcttca agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac 1561 atgaactgag gggacaggat gtcccaggcg aagggcaggg ggccaccctt ggtcaccttc 1621 agcttggcgg tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc gatctcgaac 1681 tcgtggccgt tcacggagcc ctccatgtgc accttgaagc gcatgaactc cttgatgatg 1741 gccatgttat cctcctcgcc cttgctcacc atggtggcga ccggtggatc ccgggcccgc 1801 ggtaccgtcg actgcagaat tcgaagcttg agctcgagat ctgagtccgg tagcgctagc 1861 ggatctgacg gttcactaaa ccagctctgc ttatatagac ctcccaccgt acacgcctac 1921 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt 1981 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt 2041 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga 2101 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat 2161 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat 2221 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat 2281 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg 2341 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca 2401 tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta atgcatggcg 2461 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 2521 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 2581 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 2641 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 2701 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 2761 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 2821 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 2881 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 2941 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 3001 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 3061 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 3121 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 3181 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 3241 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 3301 tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc gagccctggg 3361 ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc 3421 caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta 3481 tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg 3541 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg gtgggcgaag 3601 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt 3661 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg 3721 ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa 3781 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc 3841 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct 3901 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt 3961 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg 4021 gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt 4081 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat 4141 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg 4201 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc 4261 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag 4321 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt 4381 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca 4441 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata 4501 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa 4561 acgatcctca tcctgtctct tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct 4621 cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa 4681 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcg //