LOCUS       Exported                4180 bp ds-DNA     circular SYN 23-SEP-2022
DEFINITION  3rd generation lentiviral packaging plasmid; Contains Rev; also 
            requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid 
            (Addgene#12259).
ACCESSION   .
VERSION     .
KEYWORDS    pRSV-Rev
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D, Naldini L
  TITLE     A third-generation lentivirus vector with a conditional packaging 
            system.
  JOURNAL   J Virol. 1998 Nov . 72(11):8463-71.
  PUBMED    9765382
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 23, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     133..152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     386..403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    520..541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        556..586
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    594..610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     599..621
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     618..634
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     618..634
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        779..1040
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     1051..1075
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1169..1519
                     /codon_start=1
                     /gene="rev"
                     /product="human immunodeficiency virus 1 (HIV-1) Rev 
                     protein"
                     /label=Rev
                     /note="enhances nuclear export of unspliced and 
                     incompletely spliced viral mRNAs"
                     /translation="MAGRSGDSDEDLLKAVRLIKFLYQSNPPPNPEGTRQARRNRRRRW
                     RERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILV
                     ESPTILESGAKE"
     primer_bind     complement(1603..1620)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1603..1619)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1951..2406
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2038..2057)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2248..2269
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2423..2442)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2542..2564
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2602..2620)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2688..2792
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2793..3653
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3011..3030)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3824..232
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
       61 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      481 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
      541 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      601 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaattcga tgtacgggcc
      661 agatatacgc gtatctgagg ggactagggt gtgtttaggc gaaaagcggg gcttcggttg
      721 tacgcggtta ggagtcccct caggatatag tagtttcgct tttgcatagg gagggggaaa
      781 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
      841 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      901 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattcc
      961 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacgcc atttgaccat
     1021 tcaccacatt ggtgtgcacc tccaagctcg agctcgttta gtgaaccgtc agatcgcctg
     1081 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc
     1141 ctcgaagcta gtcgattagg catctcctat ggcaggaaga agcggagaca gcgacgaaga
     1201 cctcctcaag gcagtcagac tcatcaagtt tctctatcaa agcaacccac ctcccaatcc
     1261 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     1321 gatccattcg attagtgaac ggatccttag cacttatctg ggacgatctg cggagcctgt
     1381 gcctcttcag ctaccaccgc ttgagagact tactcttgat tgtaacgagg attgtggaac
     1441 ttctgggacg cagggggtgg gaagccctca aatattggtg gaatctccta caatattgga
     1501 gtcaggagct aaagaatagt gctgttagct tgctcaatgc cacagctata gcagtagctg
     1561 aggggacaga tagggttata gaagtagtac aagaagcttg gcactggccg tcgttttaca
     1621 acgtcgtgat ctgagcctgg gagatctctg gctaactagg gaacccactg cttaagcctc
     1681 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     1741 actagagatc aggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     1801 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     1861 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     1921 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     1981 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     2041 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     2101 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     2161 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     2221 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     2281 accctatctc gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     2341 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     2401 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     2461 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2521 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2581 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     2641 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     2701 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     2761 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     2821 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     2881 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     2941 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     3001 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     3061 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     3121 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     3181 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     3241 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     3301 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     3361 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     3421 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     3481 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     3541 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     3601 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     3661 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3721 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3781 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3841 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3901 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3961 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4021 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4081 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4141 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
//
