LOCUS Exported 5822 bp ds-DNA circular SYN 28-FEB-2023 DEFINITION VSV-G envelope expressing plasmid. ACCESSION . VERSION . KEYWORDS pMD2.G SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5822) TITLE Trono Lab Packaging and Envelope Plasmids REFERENCE 2 (bases 1 to 5822) AUTHORS . TITLE Direct Submission JOURNAL Exported Feb 28, 2023 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5822 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(311..1846) /codon_start=1 /product="vesicular stomatitis virus G glycoprotein" /label=VSV-G /note="Indiana strain" /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK" intron 1921..2396 /label=-beta-globin intron /note="internally truncated intron from human beta-globin" primer_bind complement(2509..2533) /label=LNCX /note="Human CMV promoter, forward primer" promoter complement(2530..2733) /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind complement(2559..2579) /label=CMV-F /note="Human CMV immediate early promoter, forward primer" enhancer 2734..3113 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" primer_bind 3215..3234 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 3304..3408 /gene="bla" /label=AmpR promoter CDS 3409..4269 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3627..3646) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 4440..5028 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4929..4948 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 5182..5199 /label=L4440 /note="L4440 vector, forward primer" polyA_signal 5538..110 /label=-beta-globin poly(A) /note="human beta-globin polyadenylation signal" ORIGIN 1 attaggcaga atccagatgc tcaaggccct tcataatatc ccccagttta gtagttggac 61 ttagggaaca aaggaacctt taatagaaat tggacagcaa gaaagcgagc ttagtgatac 121 ttgtgggcca gggcattagc cacaccagcc accactttct gataggcagc ctgcactggt 181 ggggtgaatt ccgttttttt tttttttttt cataaaaatt aaaaactcaa atataattga 241 ggcctctttg agcatggtat cacaagttga tttggtccaa acatgaagaa tctgttgtgc 301 aggatttgag ttactttcca agtcggttca tctctatgtc tgtataaatc tgtcttttct 361 tggtgtgctt taatttaatg caaagatgga taccaactcg gagaaccaag aatagtccaa 421 tgattaaccc tatgataaag aaaaaagagg caatagagct tttccaacta ctgaaccaac 481 cttctacaag ctcgattgga tttttggata gcccagtatc accaaaaaat aaactctcat 541 catcaggaag ttgcgaagca gcgtcttgaa tgtgaggatg ttcgaacacc tgagcctttg 601 agctaagatg aagatcggag tccaacatac catgtccaat catgtataaa ggaaacttat 661 atcctgaact ggtcctcaga actccattgg gtccaatttc cacgtcttca tatggtgccc 721 agtcatccca cagttccctt tctgtggtag ttccactgat cattccgacc attcttgaga 781 ggattggagc agcaatatcg actctgatgt atctggtctc aaagtatttt agggtaccat 841 tgattatggt gaaagcagga ccggttcctg ggtttttagg agcaagatag ctgagatcca 901 ctggagagat tggaagaccc gctctgattt tgctccaggt ttcttggcag agggaataat 961 ccaagatcct ctcaacgtcc tgaattagac ttacatccac tgaggtctga gatggagcag 1021 agatacttga cccttctggg cattcaggga atctggctgc agcaaagaga tccttatcag 1081 ccatctcgaa ccagacacct gatgggagtc tgactcccca atgcttgcag tattgcattt 1141 tgcaggcctt gcctccagtt tcataagcaa agtagttact tctgaaccct gtgccctcct 1201 ttcccaggga tgatagctct ccgtcctctg agaagaaggt gatgtccatg gaaatgaggt 1261 tagaatcaca tagccctttg accttatagt cagaatgcca ggttgtagag ttatggacag 1321 tggggcatat gtaattgctg cattttccgt tgatgaactg tgaatcaacc cattctcctg 1381 tgtattcatc aaccagcaca tggtgaggag tcacctggac aatcactgct tcggcatccg 1441 tcacagttgc atatccacaa ctttgaggag ggaagcctgg attcagccaa gttccttgtt 1501 tcgtttgttc aatgctttcc ttgcattgtt ctacagatgg agtgaaggat cggatggaat 1561 gtgttatata cttcggtcca taccagcgga aatcacaagt agtgacccat ttggaagcat 1621 gacacatcca accgtctgct tgaatagcct tgtgactctt gggcattttg acttgtaagg 1681 ctgtgcctat taagtcatta tgccaattta aatctgagct tgacgggcaa taatggtaat 1741 tagaaggaac atttttccag tttccttttt ggttgtgtgg aaaaactatg gtgaacttgc 1801 aattcacccc aatgaataaa aaggctaagt acaaaaggca cttcatagtg tcagaattca 1861 gatctcacgt gctttgccaa agtgatgggc cagcacacag accagcacgt tgcccaggag 1921 ctgtgggagg aagataagag gtatgaacat gattagcaaa agggcctagc ttggactcag 1981 aataatccag ccttatccca accataaaat aaaagcagaa tggtagctgg attgtagctg 2041 ctattagcaa tatgaaacct cttacatcag ttacaattta tatgcagaaa tatttatatg 2101 cagaaatatt gctattgcct taacccagaa attatcactg ttattcttta gaatggtgca 2161 aagaggcatg atacattgta tcattattgc cctgaaagaa agagattagg gaaagtatta 2221 gaaataagat aaacaaaaaa gtatattaaa agaagaaagc attttttaaa attacaaatg 2281 caaaattacc ctgatttggt caatatgtgt accctgttac ttctcccctt cctatgacat 2341 gaacttaacc atagaaaaga aggggaaaga aaacatcaag ggtcccatag actcaccctg 2401 aagttctcag gatccgagct cggtaccaca tgtaagcttc gaggggaggc tggatcggtc 2461 ccggtgtctt ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt 2521 cactaaacga gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt 2581 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa 2641 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc 2701 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta 2761 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg 2821 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac 2881 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat 2941 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg 3001 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg 3061 aactaatgac cccgtaattg attactatta ataactagtc aataatcaat gtcaacatgg 3121 cggtaatgtt ggacatgagc caatataaat gtacatatta tgatatggat acaacgtatg 3181 caatgggcca agctcatggc tgactaattt tttttattta tgcagaggcc gaggccggat 3241 cctctagccc atgggggccc cctcagggga tccacgtcag gtggcacttt tcggggaaat 3301 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 3361 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 3421 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 3481 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 3541 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 3601 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 3661 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 3721 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 3781 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 3841 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 3901 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 3961 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 4021 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 4081 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 4141 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 4201 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 4261 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 4321 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 4381 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 4441 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 4501 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 4561 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc 4621 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 4681 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 4741 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 4801 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 4861 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 4921 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 4981 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 5041 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 5101 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 5161 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcagatct gcggccgcac 5221 tagtgctaga ctgccatgtc gagggattcc gggtcactgt gagtggggga ggcagggaag 5281 aagggctcac aggacagtca aaccatgccc cctgtttttc cttcttcaag tagacctcta 5341 taagacaaca gagacaacta aggctgagtg gccaggcgag gagaaaccat ctcgccgtaa 5401 aacatggaag gaacacttca ggggaaaggt ggtatctcta agcaagagaa ctgagtggag 5461 tcaaggctga gagatgcagg ataagcaaat gggtagtgaa aagacattca tgaggacagc 5521 taaaacaata agtaatgtaa aatacagcat agcaaaactt taacctccaa atcaagcctc 5581 tacttgaatc cttttctgag ggatgaataa ggcataggca tcaggggctg ttgccaatgt 5641 gcattagctg tttgcagcct caccttcttt catggagttt aagatatagt gtattttccc 5701 aaggtttgaa ctagctcttc atttctttat gttttaaatg cactgacctc ccacattccc 5761 tttttagtaa aatattcaga aataatttaa atacatcatt gcaatgaaaa taaatgtttt 5821 tt //