LOCUS Exported 5046 bp ds-DNA circular SYN 21-JAN-2022 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence. . ACCESSION . VERSION . KEYWORDS pDONR221-SLC39A10_STOP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5046) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 5046) AUTHORS . TITLE Direct Submission JOURNAL Exported Jan 21, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5046 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2603..2702) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2719..2738) /label=T7 /note="T7 promoter, forward primer" promoter complement(2720..2738) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2743..2759) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2743..2759) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2861..2880) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2872..3681 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2939..2958) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3828..4416 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4317..4336 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 4570..4587 /label=L4440 /note="L4440 vector, forward primer" terminator 4746..4773 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 4865..4951 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 5000..5022 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 5014..5031 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 5015..5031 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgaa ggtgcacatg 121 cacaccaagt tctgcctgat ctgtctgctg acattcatct ttcaccactg caaccactgt 181 cacgaggagc acgatcacgg accagaggcc ctgcacaggc agcacagagg catgaccgag 241 ctggagccct ctaagttcag caagcaggcc gccgagaacg agaagaagta ctatatcgag 301 aagctgtttg agcggtacgg cgagaatggc agactgagct tctttggcct ggagaagctg 361 ctgaccaacc tgggcctggg cgagaggaag gtggtggaga tcaatcacga ggatctgggc 421 cacgaccacg tgtcccacct ggacatcctg gccgtgcagg agggcaagca ctttcactcc 481 cacaaccacc agcactctca caaccacctg aatagcgaga accagaccgt gacaagcgtg 541 tccaccaagc gcaatcacaa gtgcgatccc gagaaggaga cagtggaggt gtccgtgaag 601 tctgacgata agcacatgca cgaccacaac caccggctga gacaccacca caggctgcac 661 caccacctgg atcacaacaa tacccaccac ttccacaatg actctatcac acctagcgag 721 cgcggcgagc cttccaatga gccatctacc gagacaaaca agacccagga gcagtccgat 781 gtgaagctgc caaagggcaa gcggaagaag aagggccgca agagcaacga gaattccgaa 841 gtgatcacac ccggctttcc ccctaatcac gatcagggcg agcagtatga gcacaacagg 901 gtgcacaagc ccgaccgcgt gcacaatcct ggacactccc acgtgcacct gccagagagg 961 aacggacacg acccaggaag aggacaccag gacctggatc ctgacaatga gggcgagctg 1021 aggcacacca ggaagaggga ggcaccacac gtgaagaaca atgccatcat cagcctgaga 1081 aaggatctga acgaggacga tcaccaccac gagtgtctga atgtgacaca gctgctgaag 1141 tactatggac acggagcaaa cagcccaatc tccaccgacc tgttcacata cctgtgccct 1201 gccctgctgt atcagatcga ttcccggctg tgcatcgagc actttgacaa gctgctggtg 1261 gaggatatca ataaggacaa gaacctggtg ccagaggacg aggcaaacat cggagcatct 1321 gcctggatct gcggcatcat cagcatcacc gtgatctctc tgctgagcct gctgggcgtg 1381 atcctggtgc ctatcatcaa tcagggctgt ttcaagtttc tgctgacctt cctggtggcc 1441 ctggcagtgg gaacaatgag cggcgatgca ctgctgcacc tgctgccaca ctcccaggga 1501 ggacatgacc actctcacca gcacgcacac ggacacggac actctcacgg acacgagagc 1561 aacaagttcc tggaggagta cgatgccgtg ctgaagggac tggtggccct gggaggcatc 1621 tatctgctgt tcatcatcga gcactgcatc aggatgttta agcactacaa gcagcagcgc 1681 ggcaagcaga agtggtttat gaagcagaat accgaggaga gcacaatcgg ccggaagctg 1741 tccgaccaca agctgaacaa tacccctgat tctgactggc tgcagctgaa gccactggcc 1801 ggcacagacg attccgtggt gtctgaggat agactgaacg agaccgagct gacagacctg 1861 gagggacagc aggagagccc accaaagaat tacctgtgca tcgaggagga gaagatcatc 1921 gatcactctc acagcgacgg cctgcacacc atccacgagc acgatctgca cgcagcagca 1981 cacaatcacc acggagagaa caagacagtg ctgagaaagc acaaccacca gtggcaccac 2041 aagcacagcc accactccca cggaccatgc cactccggat ctgacctgaa ggagaccggc 2101 atcgccaaca tcgcctggat ggtcatcatg ggcgatggca tccacaattt ctccgacgga 2161 ctggcaatcg gagcagcctt ttctgccgga ctgaccggag gaatcagcac atccatcgcc 2221 gtgttctgtc acgagctgcc tcacgagctg ggcgattttg ccgtgctgct gaaggccggc 2281 atgaccgtga agcaggccat cgtgtacaat ctgctgagcg ccatgatggc ctatatcggc 2341 atgctgatcg gcacagccgt gggccagtac gccaacaata tcaccctgtg gatcttcgcc 2401 gtgacagccg gcatgtttct gtatgtggcc ctggtggaca tgctgcccga gatgctgcac 2461 ggcgatggcg acaacgagga gcacggcttc tgccctgtgg gccagtttat cctgcagaat 2521 ctgggcctgc tgttcggctt tgccatcatg ctggtcatcg ccctgtacga ggataagatc 2581 gtgttcgaca tccagtttta agacccagct ttcttgtaca aagttggcat tataagaaag 2641 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt 2701 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg 2761 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca 2821 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat 2881 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg 2941 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg 3001 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt 3061 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag 3121 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca 3181 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca 3241 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc 3301 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat 3361 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt 3421 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt 3481 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga 3541 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa 3601 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg 3661 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt 3721 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg 3781 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 3841 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 3901 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 3961 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta 4021 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 4081 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 4141 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 4201 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 4261 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 4321 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 4381 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 4441 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 4501 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 4561 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct 4621 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 4681 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa 4741 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg 4801 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg 4861 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc 4921 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta 4981 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc 5041 gggccc //