LOCUS       Exported               10317 bp ds-DNA     circular SYN 25-JAN-2022
DEFINITION  Second generation Rac1 FRET biosensor for lentivirus production.
ACCESSION   .
VERSION     .
KEYWORDS    pLenti-Rac1-2G
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10317)
  AUTHORS   Fritz RD, Menshykau D, Martin K, Reimann A, Pontelli V, Pertz O
  TITLE     SrGAP2-Dependent Integration of Membrane Geometry and 
            Slit-Robo-Repulsive Cues Regulates Fibroblast Contact Inhibition of 
            Locomotion.
  JOURNAL   Dev Cell. 2015 Sep 29. pii: S1534-5807(15)00582-1. doi: 
            10.1016/j.devcel.2015.09.002.
  PUBMED    26439400
REFERENCE   2  (bases 1 to 10317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 25, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10317
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(1286..1315)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(1325..1342)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1425..1449)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(1446..1649)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1475..1495)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1650..1953
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2029..2628)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2112..2132)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2609..2628
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2638..2967)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2652..2787
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2952..2972
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    2984..3100
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(3367..3411)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3596..3829
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4322..4447
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4494..4674
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4675..4901)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4929..4949)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4929..4947)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4968..4984)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4968..4984)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4981..5003)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4992..5008
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5016..5046)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5061..5082
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5199..5216)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5370..5958)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5450..5469)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6129..6989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6752..6771
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6990..7094)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7120..7575)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7257..7278)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7469..7488
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7702..7724
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7716..7733
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7717..7733
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7743..7762
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7743..7761
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7782..7917
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7957..8078
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7968..7987)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8022..8041
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             8150..8383
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    8474..9062
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             8580..8591
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8989..9009
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9086..9664)
                     /codon_start=1
                     /product="human Rac family small GTPase 1"
                     /label=Rac1
                     /translation="MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     primer_bind     complement(9683..9704)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(9839..40)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     10032..10051
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10291..10312
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcaccacccc ggtgaacagc tcctcgccct tgctcaccat gccaccgctg ccacccttgt
       61 acagctcgtc catgccgaga gtgatcatgg cggccgcccc tttagtagaa ccttcaccag
      121 aaccaggttt accagaacca gaagtccctt tagtagaacc ttcaccagaa ccaggtttac
      181 cagaaccaga agtcccttta gtagaacctt caccagaacc aggtttacca gaaccagaag
      241 tccctttagt agaaccttca ccagaaccag gtttaccaga accagaagtc ccgggagctg
      301 acttatctgt aaagctcatg tatttctggc tgttggatgt cttcttcgag ttgtaaaact
      361 ccaacacatc cagaacagcc tgcgggtttt tcttctgctc cgacttagtg atatttgatg
      421 tctgaagcaa gcgggcccac tgctctggca ttcccgtaaa ctcccctgtg acagcatcaa
      481 aaccgacatg aattgtgtgt tcaaaatctg aagggagaga aatctctggc cgctctttct
      541 cttttccgga gttgcgggcc acggcgctct cgtaaacggt caccttgttg tagtccttgt
      601 cgtggttcag gatctcgatg cggtggtcca caaagtgata gtcgggcagc ttcaccgcct
      661 tcttggccct gtagatggtc ttgaagtcaa cgcggtggtg gccgccgccc tccagcagca
      721 gcttgtgctt gacgtcgccc ttcagcacgc cgtcgcgcac gtacatcctc tcggtggagg
      781 cgtcccagcc ggtggtcttc ttctgcatca cggggccgtt gggggggaag ttctcgccct
      841 tgaggtgtat ctcgtagatg aaggagtcct cctccatgga gatgtcggac ttcaccttca
      901 cgatgccctt gtcctcgaag gtcatggtgc gctcccaaga gtagccctcg gggaaggact
      961 gcttgaagta gttggggatg tcgtcggggt acttggtgaa ggccctgttg ccgtaggcga
     1021 acgcggtggt cagaatgtcg taggagaagg gcaggggggc tccctccttc acctccaggt
     1081 tgatggtgtt ggtgccgtcg tagggcttgc cctcgccctc gccctcgatc acgaaggcgt
     1141 ggccattcac gttgccctcc atcttcagct tgatcttcat gtcgggcttg attacgccca
     1201 ttgtggtctc ctcgcccttg ctcaccattc ctccgctagc acccttgtac agctcgtcca
     1261 tgccgtcggt ggaggatcct gagccaagat cctcctctga tattagtttc tgctccccgc
     1321 tcccgtgatg gtggtgatgg tgtgccatgg tatatctcct ttgattgtat ctagagtcgg
     1381 tgtcttctat ggaggtcaaa acagcgtgga tggcgtctcc aggcgatctg acggttcact
     1441 aaacgagctc tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat
     1501 ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc
     1561 ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc
     1621 ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg
     1681 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat
     1741 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc
     1801 aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc
     1861 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag
     1921 ccaggcgggc catttaccgt aagttatgta acgcggaact cccaagctta tcgatgcatg
     1981 gggtcgtgcg ctcctttcgg tcgggcgctg cgggtcgtgg ggcgggcgtc aggcaccggg
     2041 cttgcgggtc atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt
     2101 gaagccgagc cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg
     2161 caccccggcg cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt
     2221 gccctggtgg tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg
     2281 gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc
     2341 ggccatgcgc gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt
     2401 ccagaccgcc accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac
     2461 gcgcgtgagg aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac
     2521 ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg
     2581 gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg taagcttttt
     2641 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     2701 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     2761 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     2821 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     2881 cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     2941 ggactttcca caccctaact gacacacatt ccacagggtc gataaaattt tgaattttgt
     3001 aatttgtttt tgtaattctt tagtttgtat gtctgttgct attatgtcta ctattctttc
     3061 ccctgcactg taccccccaa tccccccttt tcttttaaaa gttaaccgat accgtcgaga
     3121 tccgttcact aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc
     3181 ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata
     3241 tccctgccta actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa
     3301 gcctcctact atcattatga ataattttat ataccacagc caatttgtta tgttaaacca
     3361 attccacaaa cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg
     3421 gttttgcgat tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc
     3481 ccactccatc caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc
     3541 attccaaggc acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag
     3601 ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc
     3661 ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg
     3721 ctgctgcact ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc
     3781 gcccatagtg cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc
     3841 tcttttttct ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc
     3901 aatttttact actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc
     3961 tccaggtctg aagatcagcg gccgcttgct gtgcggtggt cttacttttg ttttgctctt
     4021 cctctatctt gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat
     4081 agagggttgc tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg
     4141 gttgtagctg tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa
     4201 ctgcgaatcg ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct
     4261 ttccccctgg ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac
     4321 tgacgctctc gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac
     4381 tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc
     4441 gagagagctc ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga
     4501 gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag
     4561 acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt
     4621 ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag acccgtttat
     4681 gtatcgagct aggcacttaa atacaatatc tctgcaatgc ggcaattcag tggttcgtcc
     4741 aatccatgtc agacccgtct gttgccttcc taataaggca cgatcgtacc accttacttc
     4801 caccaatcgg catgcacggt gctttttctc tccttgtaag gcatgttgct aactcatcgt
     4861 taccatgttg caagactaca agagtattgc ataagactac attaagcttg cagctccagc
     4921 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
     4981 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     5041 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     5101 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     5161 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     5221 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     5281 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     5341 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     5401 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5461 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5521 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     5581 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5641 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5701 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5761 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     5821 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5881 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5941 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6001 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6061 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     6121 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     6181 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     6241 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     6301 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     6361 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     6421 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     6481 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     6541 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     6601 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     6661 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     6721 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     6781 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     6841 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     6901 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     6961 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     7021 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     7081 ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt
     7141 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     7201 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     7261 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     7321 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     7381 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     7441 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     7501 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     7561 cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     7621 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     7681 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg
     7741 cgtaatacga ctcactatag ggcgaattgg gtacgtccct aggcctccaa aaaagcctcc
     7801 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     7861 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     7921 cggagttagg ggcgggatag ctagagccag acatgataag atacattgat gagtttggac
     7981 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     8041 ctttatttgt aaccattata agctgcaata aacaagttcc tctcactctc tgatattcat
     8101 ttctttgcaa gttataaata ctgaataata agatgacatg aactactact gctagagatt
     8161 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg
     8221 gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct
     8281 agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc agtacaagca
     8341 aaaagcagat cttgtcttcg ttgggagtga attagccctt ccagtccccc cttttctttt
     8401 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg taccgagctc
     8461 gaattaattc caggcgggga ggcggcccaa agggagatcc gactcgtctg agggcgaagg
     8521 cgaagacgcg gaagaggccg cagagccggc agcaggccgc gggaaggaag gtccgctgga
     8581 ttgagggccg aagggacgta gcagaaggac gtcccgcgca gaatccaggt ggcaacacag
     8641 gcgagcagcc atggaaagga cgtcagcttc cccgacaaca ccacggaatt gtcagtgccc
     8701 aacagccgag cccctgtcca gcagcgggca aggcaggcgg cgatgagttc cgccgtggca
     8761 atagggaggg ggaaagcgaa agtcccggaa aggagctgac aggtggtggc aatgccccaa
     8821 ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct gacaacgggc
     8881 cacaactcct cataaagaga cagcaaccag gatttataca aggaggagaa aatgaaagcc
     8941 atacgggaag caatagcatg atacaaaggc attaaagcag cgtatccaca tagcgtaaaa
     9001 ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc cagaggttga
     9061 ttgtcgacga attctggtgc tcgagtcaca acagcaggca ttttctcttc ctcttcttca
     9121 cgggaggcgg gcagaggact gctcggatcg cttcgtcaaa cactgtcttg aggcctcgct
     9181 gtgtgagcgc cgagcactcc aggtatttta cagcaccaat ctccttggcc atagctagac
     9241 cctgcggata ggtgatggga gtcagcttct tctccttcag tttctcgatc gtgtctttat
     9301 catccctaag atcaagttta gttcccacta ggatgatggg agtgttggga cagtggtgcc
     9361 gcacctcagg ataccacttt gcacggacat tttcaaatga tgcaggactc acaagggaaa
     9421 agcaaattaa gaacacatct gtttgcggat aggatagggg gcgtaatctg tcataatctt
     9481 cttgtccagc tgtatcccat aagcccagat tcaccggttt tccatctacc ataacattgg
     9541 cagaataatt gtcaaagaca gtagggatat attctccagg aaatgcattg gttgtgtaac
     9601 tgatcagtag gcaagtttta cctacagctc cgtctcccac caccacacac ttgatggcct
     9661 gcatggtacc cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
     9721 tggggtcttt gctcagggcg gactggtagc tcaggtagtg gttgtcgggc agcagcacgg
     9781 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ccgccgtcct
     9841 cgatgttgtg gcggatcttg aagttggcct tgatgccgtt cttctgcttg tcggcggtga
     9901 tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct
     9961 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca
    10021 cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt
    10081 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggg
    10141 cgaagcacat caggccgtag cccagggtgg tcacgagggt gggccagggc acgggcagct
    10201 tgccggtggt gcagatcagc ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
    10261 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgg
//
