LOCUS Exported 5676 bp ds-DNA circular SYN 26-JAN-2022 DEFINITION Localization: Microtubules, Excitation: 587, Emission: 610. ACCESSION . VERSION . KEYWORDS mCherry-MAPTau-C-10 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5676) TITLE Davidson lab - mCherry constructs REFERENCE 2 (bases 1 to 5676) AUTHORS . TITLE Direct Submission JOURNAL Exported Jan 26, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5676 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 547..556 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 553..1260 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(698..716) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 966..985 /label=mCherry-F /note="mCherry, forward primer" polyA_signal 2476..2597 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2513..2532) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2567..2586 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2604..3059) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2741..2762) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2953..2972 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3086..3190 /gene="bla" /label=AmpR promoter primer_bind complement(3215..3235) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 3259..3469 /label=SV40 promoter /note="SV40 early promoter" promoter 3281..3477 /label=SV40 promoter /note="SV40 early promoter" rep_origin 3328..3463 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3390..3409 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3512..4306 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3566..3585) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4176..4195 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4494..4513) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4538..4585 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4914..5502 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5403..5422 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 541 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 601 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 661 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 721 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 781 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 841 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 901 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 961 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 1021 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 1081 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 1141 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 1201 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 1261 tccggaagcg gcgggtccgg gagcggaagc gctgagcccc gccaggagtt cgaagtgatg 1321 gaagatcacg ctgggacgta cgggttgggg gacaggaaag atcagggggg ctacaccatg 1381 caccaagacc aagagggtga cacggacgct ggcctgaaag ctgaagaagc aggcattgga 1441 gacaccccca gcctggaaga cgaagctgct ggtcacgtga cccaagctcg catggtcagt 1501 aaaagcaaag acgggactgg aagcgatgac aaaaaagcca agggggctga tggtaaaacg 1561 aagatcgcca caccgcgggg agcagcccct ccaggccaga agggccaggc caacgccacc 1621 aggattccag caaaaacccc gcccgctcca aagacaccac ccagctctgg tgaacctcca 1681 aaatcagggg atcgcagcgg ctacagcagc cccggctccc caggcactcc cggcagccgc 1741 tcccgcaccc cgtcccttcc aaccccaccc acccgggagc ccaagaaggt ggcagtggtc 1801 cgtactccac ccaagtcgcc gtcttccgcc aagagccgcc tgcagacagc ccccgtgccc 1861 atgccagacc tgaagaatgt caagtccaag atcggctcca ctgagaacct gaagcaccag 1921 ccgggaggcg ggaaggtgca aatagtctac aaaccagttg acctgagcaa ggtgacctcc 1981 aagtgtggct cattaggcaa catccatcat aaaccaggag gtggccaggt ggaagtaaaa 2041 tctgagaagc ttgacttcaa ggacagagtc cagtcgaaga ttgggtccct ggacaatatc 2101 acccacgtcc ctggcggagg aaataaaaag attgaaaccc acaagctgac cttccgcgag 2161 aacgccaaag ccaagacaga ccacggggcg gagatcgtgt acaagtcgcc agtggtgtct 2221 ggggacacgt ctccacggca tctcagcaat gtctcctcca ccggcagcat cgacatggta 2281 gactcgcccc agctcgccac gctagctgac gaggtgtctg cctccctggc caagcagggt 2341 ttgtgaggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag 2401 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat 2461 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc 2521 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 2581 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt 2641 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta 2701 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc 2761 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg 2821 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact 2881 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt 2941 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc 3001 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc 3061 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 3121 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 3181 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga 3241 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 3301 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 3361 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 3421 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat 3481 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg 3541 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 3601 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 3661 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct 3721 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 3781 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 3841 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 3901 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 3961 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 4021 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 4081 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 4141 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 4201 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 4261 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg 4321 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc 4381 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc 4441 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca 4501 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa 4561 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct 4621 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca 4681 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc 4741 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa 4801 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 4861 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 4921 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 4981 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 5041 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 5101 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 5161 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 5221 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 5281 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 5341 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 5401 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 5461 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 5521 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 5581 cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac 5641 ggggtcatta gttcatagcc catatatgga gttccg //