LOCUS       Exported                9952 bp ds-DNA     circular SYN 18-FEB-2022
DEFINITION  Lentiviral expression of Cre recombinase and mCherry reporter from 
            CMV promoter (separated by P2A peptide), and blasticidin resistance 
            gene for selection in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    pLenti-mCherry-Cre-blast
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9952)
  AUTHORS   Sapozhnikov DM, Szyf M
  TITLE     Unraveling the functional role of DNA demethylation at specific 
            promoters by targeted steric blockage of DNA methyltransferase with 
            CRISPR/dCas9.
  JOURNAL   Nat Commun. 2021 Sep 29;12(1):5711. doi: 10.1038/s41467-021-25991-9.
  PUBMED    34588447
REFERENCE   2  (bases 1 to 9952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 18, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9952
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     mobile_element  1..768
                     /mobile_element_type="insertion sequence:IS1"
                     /note="prokaryotic transposable element"
     promoter        809..1035
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1036..1216
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1263..1388
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1881..2114
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2299..2343
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2622..2925
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2926..3129
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3080..3100
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3167..3874
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3312..3330)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3580..3599
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3884..3940
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3941..4972
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     primer_bind     complement(5032..5052)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5450..5461)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5491..5532
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(5569..5589)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5574..5903
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5754..5889
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5951..5998
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6017..6415
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6502..6735
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6807..6928
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6844..6863)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6898..6917
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6968..7103
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7123..7142)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7124..7142)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7152..7169)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7152..7168)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7161..7183)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7310..7765
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7397..7416)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7607..7628
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7791..7895
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7896..8756
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8114..8133)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8927..9515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9416..9435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9669..9686
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9803..9824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9839..9869
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9877..9893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9882..9904
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9901..9917
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9901..9917
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ggtaatgact ccaacttatt gatagtgttt tatgttcaga taatgcccga tgactttgtc
       61 atgcagctcc accgattttg agaacgacag cgacttccgt cccagccgtg ccaggtgctg
      121 cctcagattc aggttatgcc gctcaattcg ctgcgtatat cgcttgctga ttacgtgcag
      181 ctttcccttc aggcgggatt catacagcgg ccagccatcc gtcatccata tcaccacgtc
      241 aaagggtgac agcaggctca taagacgccc cagcgtcgcc atagtgcgtt caccgaatac
      301 gtgcgcaaca accgtcttcc ggagactgtc atacgcgtaa aacagccagc gctggcgcga
      361 tttagccccg acatagcccc actgttcgtc catttccgcg cagacgatga cgtcactgcc
      421 cggctgtatg cgcgaggtta ccgactgcgg cctgagtttt ttaagtgacg taaaatcgtg
      481 ttgaggccaa cgcccataat gcgggctgtt gcccggcatc caacgccatt catggccata
      541 tcaatgattt tctggtgcgt accgggttga gaagcggtgt aagtgaactg cagttgccat
      601 gttttacggc agtgagagca gagatagcgc tgatgtccgg cggtgctttt gccgttacgc
      661 accaccccgt cagtagctga acaggaggga cagctgatag aaacagaagc cactggagca
      721 cctcaaaaac accatcatac actaaatcag taagttggca gcatcacccc ctcactaaag
      781 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
      841 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
      901 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
      961 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
     1021 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     1081 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     1141 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     1201 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     1261 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     1321 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     1381 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     1441 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     1501 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     1561 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     1621 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     1681 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     1741 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     1801 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     1861 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     1921 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     1981 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     2041 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     2101 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     2161 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     2221 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     2281 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     2341 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     2401 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     2461 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     2521 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     2581 gtgaacggat ctcgacggta tcgataagct tgggagttcc gcgttacata acttacggta
     2641 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     2701 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     2761 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     2821 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     2881 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     2941 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     3001 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     3061 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     3121 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtatgg tgagcaaggg
     3181 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     3241 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     3301 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     3361 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     3421 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     3481 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     3541 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     3601 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     3661 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     3721 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     3781 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     3841 ccactccacc ggcggcatgg acgagctgta caagggatct ggagcaacaa acttctcact
     3901 actcaaacaa gcaggtgacg tggaggagaa tcccggccct atgtccaatt tactgaccgt
     3961 acaccaaaat ttgcctgcat taccggtcga tgcaacgagt gatgaggttc gcaagaacct
     4021 gatggacatg ttcagggatc gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc
     4081 cgtttgccgg tcgtgggcgg catggtgcaa gttgaataac cggaaatggt ttcccgcaga
     4141 acctgaagat gttcgcgatt atcttctata tcttcaggcg cgcggtctgg cagtaaaaac
     4201 tatccagcaa catttgggcc agctaaacat gcttcatcgt cggtccgggc tgccacgacc
     4261 aagtgacagc aatgctgttt cactggttat gcggcggatc cgaaaagaaa acgttgatgc
     4321 cggtgaacgt gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc aggttcgttc
     4381 actcatggaa aatagcgatc gctgccagga tatacgtaat ctggcatttc tggggattgc
     4441 ttataacacc ctgttacgta tagccgaaat tgccaggatc agggttaaag atatctcacg
     4501 tactgacggt gggagaatgt taatccatat tggcagaacg aaaacgctgg ttagcaccgc
     4561 aggtgtagag aaggcactta gcctgggggt aactaaactg gtcgagcgat ggatttccgt
     4621 ctctggtgta gctgatgatc cgaataacta cctgttttgc cgggtcagaa aaaatggtgt
     4681 tgccgcgcca tctgccacca gccagctatc aactcgcgcc ctggaaggga tttttgaagc
     4741 aactcatcga ttgatttacg gcgctaagga tgactctggt cagagatacc tggcctggtc
     4801 tggacacagt gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag tttcaatacc
     4861 ggagatcatg caagctggtg gctggaccaa tgtaaatatt gtcatgaact atatccgtaa
     4921 cctggatagt gaaacagggg caatggtgcg cctgctggaa gatggcgatt aggtcgacaa
     4981 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     5041 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     5101 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     5161 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     5221 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     5281 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     5341 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     5401 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     5461 tccagcggac cttccttccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt
     5521 ctcgattcta cgcgtaccgg ttagtaatga gtttggaatt aattctgtgg aatgtgtgtc
     5581 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5641 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5701 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     5761 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5821 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     5881 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     5941 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     6001 ggtgaggaac taaaccatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
     6061 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
     6121 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     6181 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     6241 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     6301 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
     6361 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaagcaca
     6421 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     6481 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     6541 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6601 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6661 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6721 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     6781 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     6841 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     6901 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     6961 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7021 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7081 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     7141 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     7201 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     7261 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     7321 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7381 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     7441 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     7501 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     7561 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7621 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7681 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     7741 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     7801 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     7861 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     7921 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     7981 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     8041 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     8101 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     8161 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     8221 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     8281 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     8341 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     8401 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     8461 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     8521 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     8581 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     8641 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     8701 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     8761 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     8821 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     8881 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     8941 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     9001 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     9061 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     9121 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     9181 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     9241 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     9301 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     9361 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     9421 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     9481 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     9541 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     9601 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     9661 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     9721 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     9781 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     9841 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     9901 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac ta
//
