LOCUS       Exported                8097 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBMN YFP-LID
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Bonger KM, Chen LC, Liu CW, Wandless TJ
  TITLE     Small-molecule displacement of a cryptic degron causes conditional 
            protein degradation.
  JOURNAL   Nat Chem Biol. 2011 Jul 3. doi: 10.1038/nchembio.598.
  PUBMED    21725303
REFERENCE   2  (bases 1 to 8097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..594
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    657..1014
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1079..1495
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1425..1447
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1463..1479
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1505..1878
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1816..1832
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             1896..1922
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1923..2639
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1968..1989)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2229..2248)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2576..2597
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2646..2966
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     misc_feature    3067..3652
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3234..3251)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3461..3480
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3653..4336
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     CDS             4349..5035
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     LTR             5084..5677
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5808..5825)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5979..6567)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6059..6078)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6741..7601)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7364..7383
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     7669..7687
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaaat acataactga gaatagaaaa gttcagatca aggtcaggaa cagatggaac
      121 agctgaatat gggccaaagc ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
      181 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
      241 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
      301 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
      361 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
      421 ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg
      481 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
      541 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg
      601 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
      661 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc
      721 ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
      781 gagttcggaa cacccggccg caaccctggg agacgtccca gggacttcgg gggccgtttt
      841 tgtggcccga cctgagtcca aaaatcccga tcgttttgga ctctttggtg cacccccctt
      901 agaggaggga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
      961 tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat
     1021 cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaata tgggcccggg
     1081 ccagactgtt accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat
     1141 cgctcacaac cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga
     1201 atggccaacc tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac
     1261 ccaggttaag atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta
     1321 catcgtgacc tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca
     1381 ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg
     1441 ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag gcgcccccat
     1501 atggccatat gagatcttat atggggcacc cccgcccctt gtaaacttcc ctgaccctga
     1561 catgacaaga gttactaaca gcccctctct ccaagctcac ttacaggctc tctacttagt
     1621 ccagcacgaa gtctggagac ctctggcggc acgtaccaag aacaactgga ccgaccggtg
     1681 gtacctcacc cttaccgagt cggcgacaca gtgtgggtcc gccgacacca gactaagaac
     1741 ctagaacctc gctggaaagg accttacaca gtcctgctga ccacccccac cgccctcaaa
     1801 gtagacggca tcgcagcttg gatacacgcc gcccacgtga aggctgccga ccccgggggt
     1861 ggaccatcct ctagactgcc ggatccgcca ccatgtatcc gtacgacgta ccggattatg
     1921 cgatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     1981 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     2041 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     2101 ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
     2161 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     2221 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     2281 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     2341 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     2401 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     2461 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     2521 actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     2581 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     2641 aattcggagt gcaggtggaa accatctccc caggagacgg gcgcaccttc cccaagcgcg
     2701 gccagacctg cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa gttgattcct
     2761 cccgggacag aaacaagccc tttaagttta tgctaggcaa gcaggaggtg atccgaggct
     2821 gggaagaagg ggttgcccag atgagtgtgg gtcagagagc caaactgact atatctccag
     2881 attatgccta tggtgccact gggcacccag gcatcatccc accacatgcc actctcgtct
     2941 tcgatgtgga gcttctaaaa ctggaaacgc gtggagtgga ggaagtggcg gagggggtag
     3001 ttctcttaag gcggcgaggt aattagctga atgcatcgat ccgcggcggc cgctacgtaa
     3061 attccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     3121 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     3181 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     3241 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     3301 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     3361 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     3421 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     3481 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     3541 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac
     3601 cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatggtgag
     3661 cggcctgctg aaggagagta tgcgcatcaa gatgtacatg gagggcaccg tgaacggcca
     3721 ctacttcaag tgcgagggcg agggcgacgg caaccccttc gccggcaccc agagcatgag
     3781 aatccacgtg accgagggcg cccccctgcc cttcgccttc gacatcctgg ccccctgctg
     3841 cgagtacggc agcaggacct tcgtgcacca caccgccgag atccccgact tcttcaagca
     3901 gagcttcccc gagggcttca cctgggagag aaccaccacc tacgaggacg gcggcatcct
     3961 gaccgcccac caggacacca gcctggaggg caactgcctg atctacaagg tgaaggtgca
     4021 cggcaccaac ttccccgccg acggccccgt gatgaagaac aagagcggcg gctgggagcc
     4081 cagcaccgag gtggtgtacc ccgagaacgg cgtgctgtgc ggccggaacg tgatggccct
     4141 gaaggtgggc gaccggcacc tgatctgcca ccactacacc agctaccgga gcaagaaggc
     4201 cgtgcgcgcc ctgaccatgc ccggcttcca cttcaccgac atccggctcc agatgctgcg
     4261 gaagaagaag gacgagtact tcgagctgta cgaggccagc gtggcccggt acagcgacct
     4321 gcccgagaag gccaacagat ctcccgggat ggtgagcggc ctgctgaagg agagtatgcg
     4381 catcaagatg tacatggagg gcaccgtgaa cggccactac ttcaagtgcg agggcgaggg
     4441 cgacggcaac cccttcgccg gcacccagag catgagaatc cacgtgaccg agggcgcccc
     4501 cctgcccttc gccttcgaca tcctggcccc ctgctgcgag tacggcagca ggaccttcgt
     4561 gcaccacacc gccgagatcc ccgacttctt caagcagagc ttccccgagg gcttcacctg
     4621 ggagagaacc accacctacg aggacggcgg catcctgacc gcccaccagg acaccagcct
     4681 ggagggcaac tgcctgatct acaaggtgaa ggtgcacggc accaacttcc ccgccgacgg
     4741 ccccgtgatg aagaacaaga gcggcggctg ggagcccagc accgaggtgg tgtaccccga
     4801 gaacggcgtg ctgtgcggcc ggaacgtgat ggccctgaag gtgggcgacc ggcacctgat
     4861 ctgccaccac tacaccagct accggagcaa gaaggccgtg cgcgccctga ccatgcccgg
     4921 cttccacttc accgacatcc ggctccagat gctgcggaag aagaaggacg agtacttcga
     4981 gctgtacgag gccagcgtgg cccggtacag cgacctgccc gagaaggcca actgagtcga
     5041 cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
     5101 gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac
     5161 tgagaataga gaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa
     5221 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag
     5281 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     5341 gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt
     5401 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
     5461 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
     5521 ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa
     5581 taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc
     5641 tgagtgattg actacccgtc agcgggggtc tttcattcac atgcagcatg tatcaaaatt
     5701 aatttggttt tttttcttaa gtatttacat taaatggcca tagttgcatt aatgaatcgg
     5761 ccaacgcgcg gggagaggcg gtttgcgtat tggcgctctt ccgcttcctc gctcactgac
     5821 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     5881 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     5941 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6001 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6061 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6121 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6181 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6241 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6301 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6361 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     6421 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6481 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6541 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     6601 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     6661 ttcacctaga tccttttaaa ttaaaaatga agtttgcgca aatcaatcta aagtatatat
     6721 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6781 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6841 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6901 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6961 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7021 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7081 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7141 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7201 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7261 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7321 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
     7381 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7441 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7501 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7561 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7621 tattgaagca tttatcaggg ttattgtctc atgacattaa cctataaaaa taggcgtatc
     7681 acgaggccct ttcgtcttca agaacagctt tgctcttagg agtttcctaa tacatcccaa
     7741 actcaaatat ataaagcatt tgacttgttc tatgccctag ggggcggggg gaagctaagc
     7801 cagctttttt taacatttaa aatgttaatt ccattttaaa tgcacagatg tttttatttc
     7861 ataagggttt caatgtgcat gaatgctgca atattcctgt taccaaagct agtataaata
     7921 aaaatagata aacgtggaaa ttacttagag tttctgtcat taacgtttcc ttcctcagtt
     7981 gacaacataa atgcgctgct gagaagccag tttgcatctg tcaggatcaa tttcccatta
     8041 tgccagtcat attaattact agtcaattag ttgattttta tttttgacat atacatg
//
