LOCUS       Exported                9983 bp ds-DNA     circular SYN 22-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pRosa26PAm1 (DM#272)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9983)
  AUTHORS   Murtaugh LC, Stanger BZ, Kwan KM, Melton DA
  TITLE     Notch signaling controls multiple steps of pancreatic 
            differentiation.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Dec 9. 100(25):14920-5.
  PUBMED    14657333
REFERENCE   2  (bases 1 to 9983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 22, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9983
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    6..1088
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    1116..5456
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        5506..6005
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5541..5564)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5938..5957
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6032..6604
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          6681..6746
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(6814..6831)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6820..7044
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7138..7158)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7138..7156)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7177..7193)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7177..7193)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7190..7212)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7201..7217
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7225..7255)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7270..7291
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7408..7425)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7579..8167)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7659..7678)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8338..9198)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8961..8980
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9199..9303)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9330..9785
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9417..9436)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9627..9648
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9911..9933
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9925..9942
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9926..9942
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9952..9971
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9952..9970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccaccgcggc aggccctccg agcgtggtgg agccgttctg tgagacagcc gggtacgagt
       61 cgtgacgctg gaaggggcaa gcgggtggtg ggcaggaatg cggtccgccc tgcagcaacc
      121 ggagggggag ggagaaggga gcggaaaagt ctccaccgga cgcggccatg gctcgggggg
      181 gggggggcag cggaggagcg cttccggccg acgtctcgtc gctgattggc ttcttttcct
      241 cccgccgtgt gtgaaaacac aaatggcgtg ttttggttgg cgtaaggcgc ctgtcagtta
      301 acggcagccg gagtgcgcag ccgccggcag cctcgctctg cccactgggt ggggcgggag
      361 gtaggtgggg tgaggcgagc tggacgtgcg ggcgcggtcg gcctctggcg gggcggggga
      421 ggggagggag ggtcagcgaa agtagctcgc gcgcgagcgg ccgcccaccc tccccttcct
      481 ctgggggagt cgttttaccc gccgccggcc gggcctcgtc gtctgattgg ctctcggggc
      541 ccagaaaact ggcccttgcc attggctcgt gttcgtgcaa gttgagtcca tccgccggcc
      601 agcgggggcg gcgaggaggc gctcccaggt tccggccctc ccctcggccc cgcgccgcag
      661 agtctggccg cgcgcccctg cgcaacgtgg caggaagcgc gcgctggggg cggggacggg
      721 cagtagggct gagcggctgc ggggcgggtg caagcacgtt tccgacttga gttgcctcaa
      781 gaggggcgtg ctgagccaga cctccatcgc gcactccggg gagtggaggg aaggagcgag
      841 ggctcagttg ggctgttttg gaggcaggaa gcacttgctc tcccaaagtc gctctgagtt
      901 gttatcagta agggagctgc agtggagtag gcggggagaa ggccgcaccc ttctccggag
      961 gggggagggg agtgttgcaa tacctttctg ggagttctct gctgcctcct ggcttctgag
     1021 gaccgccctg ggcctgggag aatcccttcc ccctcttccc tcgtgatctg caactccagt
     1081 ctttctagcc ttaattaatt taaatggcgc gccggctaga agatgggcgg gagtcttctg
     1141 ggcaggctta aaggctaacc tggtgtgtgg gcgttgtcct gcaggggaat tgaacaggtg
     1201 taaaattgga gggacaagac ttcccacaga ttttcggttt tgtcgggaag ttttttaata
     1261 ggggcaaata aggaaaatgg gaggataggt agtcatctgg ggttttatgc agcaaaacta
     1321 caggttatta ttgcttgtga tccgcctcgg agtattttcc atcgaggtag attaaagaca
     1381 tgctcacccg agttttatac tctcctgctt gagatcctta ctacagtatg aaattacagt
     1441 gtcgcgagtt agactatgta agcagaattt taatcatttt taaagagccc agtacttcat
     1501 atccatttct cccgctcctt ctgcagcctt atcaaaaggt attttagaac actcatttta
     1561 gccccatttt catttattat actggcttat ccaaccccta gacagagcat tggcattttc
     1621 cctttcctga tcttagaagt ctgatgactc atgaaaccag acagattagt tacatacacc
     1681 acaaatcgag gctgtagctg gggcctcaac actgcagttc ttttataact ccttagtaca
     1741 ctttttgttg atcctttgcc ttgatcctta attttcagtg tctatcacct ctcccgtcag
     1801 gtggtgttcc acatttgggc ctattctcag tccagggagt tttacaacaa tagatgtatt
     1861 gagaatccaa cctaaagctt aactttccac tcccatgaat gcctctctcc tttttctcca
     1921 tttataaact gagctattaa ccattaatgg tttccaggtg gatgtctcct cccccaatat
     1981 tacctgatgt atcttacata ttgccaggct gatattttaa gacattaaaa ggtatatttc
     2041 attattgagc cacatggtat tgattactgc ttactaaaat tttgtcattg tacacatctg
     2101 taaaaggtgg ttccttttgg aatgcaaagt tcaggtgttt gttgtctttc ctgacctaag
     2161 gtcttgtgag cttgtatttt ttctatttaa gcagtgcttt ctcttggact ggcttgactc
     2221 atggcattct acacgttatt gctggtctaa atgtgatttt gccaagcttc ttcaggacct
     2281 ataattttgc ttgacttgta gccaaacaca agtaaaatga ttaagcaaca aatgtatttg
     2341 tgaagcttgg tttttaggtt gttgtgttgt gtgtgcttgt gctctataat aatactatcc
     2401 aggggctgga gaggtggctc ggagttcaag agcacagact gctcttccag aagtcctgag
     2461 ttcaattccc agcaaccaca tggtggctca caaccatctg taatgggatc tgatgccctc
     2521 ttctggtgtg tctgaagacc acaagtgtat tcacattaaa taaataaatc ctccttcttc
     2581 ttcttttttt tttttttaaa gagaatactg tctccagtag aatttactga agtaatgaaa
     2641 tactttgtgt ttgttccaat atggtagcca ataatcaaat tactctttaa gcactggaaa
     2701 tgttaccaag gaactaattt ttatttgaag tgtaactgtg gacagaggag ccataactgc
     2761 agacttgtgg gatacagaag accaatgcag actttaatgt cttttctctt acactaagca
     2821 ataaagaaat aaaaattgaa cttctagtat cctatttgtt taaactgcta gctttactta
     2881 acttttgtgc ttcatctata caaagctgaa agctaagtct gcagccatta ctaaacatga
     2941 aagcaagtaa tgataatttt ggatttcaaa aatgtagggc cagagtttag ccagccagtg
     3001 gtggtgcttg cctttatgcc tttaatccca gcactctgga ggcagagaca ggcagatctc
     3061 tgagtttgag cccagcctgg tctacacatc aagttctatc taggatagcc aggaatacac
     3121 acagaaaccc tgttggggag gggggctctg agatttcata aaattataat tgaagcattc
     3181 cctaatgagc cactatggat gtggctaaat ccgtctacct ttctgatgag atttgggtat
     3241 tattttttct gtctctgctg ttggttgggt cttttgacac tgtgggcttt ctttaaagcc
     3301 tccttcctgc catgtggtct cttgtttgct actaacttcc catggcttaa atggcatggc
     3361 tttttgcctt ctaagggcag ctgctgagat ttgcagcctg atttccaggg tggggttggg
     3421 aaatctttca aacactaaaa ttgtccttta attttttttt taaaaaatgg gttatataat
     3481 aaacctcata aaatagttat gaggagtgag gtggactaat attaaatgag tccctcccct
     3541 ataaaagagc tattaaggct ttttgtctta tacttaactt tttttttaaa tgtggtatct
     3601 ttagaaccaa gggtcttaga gttttagtat acagaaactg ttgcatcgct taatcagatt
     3661 ttctagtttc aaatccagag aatccaaatt cttcacagcc aaagtcaaat taagaatttc
     3721 tgacttttaa tgttaatttg cttactgtga atataaaaat gatagctttt cctgaggcag
     3781 ggtctcacta tgtatctctg cctgatctgc aacaagatat gtagactaaa gttctgcctg
     3841 cttttgtctc ctgaatacta aggttaaaat gtagtaatac ttttggaact tgcaggtcag
     3901 attcttttat aggggacaca ctaagggagc ttgggtgata gttggtaaaa tgtgtttcaa
     3961 gtgatgaaaa cttgaattat tatcaccgca acctactttt taaaaaaaaa agccaggcct
     4021 gttagagcat gcttaaggga tccctaggac ttgctgagca cacaagagta gttacttggc
     4081 aggctcctgg tgagagcata tttcaaaaaa caaggcagac aaccaagaaa ctacagttaa
     4141 ggttacctgt ctttaaacca tctgcatata cacagggata ttaaaatatt ccaaataata
     4201 tttcattcaa gttttccccc atcaaattgg gacatggatt tctccggtga ataggcagag
     4261 ttggaaacta aacaaatgtt ggttttgtga tttgtgaaat tgttttcaag tgatagttaa
     4321 agcccatgag atacagaaca aagctgctat ttcgaggtct cttggtttat actcagaagc
     4381 acttctttgg gtttccctgc actatcctga tcatgtgcta ggcctacctt aggctgattg
     4441 ttgttcaaat aaacttaagt ttcctgtcag gtgatgtcat atgatttcat atatcaaggc
     4501 aaaacatgtt atatatgtta aacatttgta cttaatgtga aagttaggtc tttgtgggtt
     4561 tgatttttaa ttttcaaaac ctgagctaaa taagtcattt ttacatgtct tacatttggt
     4621 ggaattgtat aattgtggtt tgcaggcaag actctctgac ctagtaaccc tacctataga
     4681 gcactttgct gggtcacaag tctaggagtc aagcatttca ccttgaagtt gagacgtttt
     4741 gttagtgtat actagtttat atgttggagg acatgtttat ccagaagata ttcaggacta
     4801 tttttgactg ggctaaggaa ttgattctga ttagcactgt tagtgagcat tgagtggcct
     4861 ttaggcttga attggagtca cttgtatatc tcaaataatg ctggcctttt ttaaaaagcc
     4921 cttgttcttt atcaccctgt tttctacata atttttgttc aaagaaatac ttgtttggat
     4981 ctccttttga caacaatagc atgttttcaa gccatatttt ttttcctttt tttttttttt
     5041 tttggttttt cgagacaggg tttctctgta tagccctggc tgtcctggaa ctcactttgt
     5101 agaccaggct ggcctcgaac tcagaaatcc gcctgcctct gcctcctgag tgccgggatt
     5161 aaaggcgtgc accaccacgc ctggctaagt tggatatttt gttatataac tataaccaat
     5221 actaactcca ctgggtggat ttttaattca gtcagtagtc ttaagtggtc tttattggcc
     5281 cttcattaaa atctactgtt cactctaaca gaggctgttg gtactagtgg cacttaagca
     5341 acttcctacg gatatactag cagattaagg gtcagggata gaaactagtc tagcgttttg
     5401 tatacctacc agctttatac taccttgttc tgatagaaat atttcaggac atctagctta
     5461 tcgataccgt cgacggtatc gataagcttg atatcgaatt ctaccgggta ggggaggcgc
     5521 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     5581 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     5641 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     5701 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     5761 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     5821 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     5881 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     5941 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     6001 tttcgacctg caggtcctcg ccatggatcc tgatgatgtt gttgattctt ctaaatcttt
     6061 tgtgatggaa aacttttctt cgtaccacgg gactaaacct ggttatgtag attccattca
     6121 aaaaggtata caaaagccaa aatctggtac acaaggaaat tatgacgatg attggaaagg
     6181 gttttatagt accgacaata aatacgacgc tgcgggatac tctgtagata atgaaaaccc
     6241 gctctctgga aaagctggag gcgtggtcaa agtgacgtat ccaggactga cgaaggttct
     6301 cgcactaaaa gtggataatg ccgaaactat taagaaagag ttaggtttaa gtctcactga
     6361 accgttgatg gagcaagtcg gaacggaaga gtttatcaaa aggttcggtg atggtgcttc
     6421 gcgtgtagtg ctcagccttc ccttcgctga ggggagttct agcgttgaat atattaataa
     6481 ctgggaacag gcgaaagcgt taagcgtaga acttgagatt aattttgaaa cccgtggaaa
     6541 acgtggccaa gatgcgatgt atgagtatat ggctcaagcc tgtgcaggaa atcgtgtcag
     6601 gcgatctctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
     6661 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
     6721 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
     6781 ggtggaatgc agatcctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     6841 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     6901 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     6961 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     7021 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgaggg
     7081 ggggcccggt acatttaaat gcgatcgcgt ttaaacgcgt gtacccagct tttgttccct
     7141 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7201 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7261 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7321 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     7381 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7441 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7501 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7561 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7621 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7681 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7741 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7801 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7861 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7921 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7981 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     8041 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8101 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8161 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8221 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8281 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8341 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8401 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8461 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8521 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8581 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8641 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8701 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     8761 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     8821 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     8881 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     8941 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9001 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9061 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9121 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9181 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9241 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9301 gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc
     9361 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     9421 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     9481 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     9541 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     9601 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     9661 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     9721 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     9781 aatttccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     9841 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     9901 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
     9961 tcactatagg gcgaattgga gct
//
