LOCUS       Exported                9229 bp ds-DNA     circular SYN 29-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Cre-IRES-PuroR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Somers A, Jean JC, Sommer CA, Omari A, Ford CC, Mills JA, Ying L, 
            Sommer AG, Jean JM, Smith BW, Lafyatis R, Demierre MF, Weiss DJ, 
            French DL, Gadue P, Murphy GJ, Mostoslavsky G, Kotton DN
  TITLE     Generation of transgene-free lung disease-specific human induced 
            pluripotent stem cells using a single excisable lentiviral stem cell
            cassette.
  JOURNAL   Stem Cells. 2010 Oct . 28(10):1728-40.
  PUBMED    20715179
REFERENCE   2  (bases 1 to 9229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 29, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9229
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     32..51
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     285..302
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     394..413
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    542..563
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        578..608
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    616..632
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     621..643
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     640..656
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     640..656
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     706..725
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             917..1550
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1597..1722
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2219..2452
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2637..2681
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2974..3091
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3139..4317
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3370..4308
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4265..4285
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4322..4331
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4361..5389
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    5393..5960
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5410..5962
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5554..5571)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5781..5800
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5974..6573
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5974..5993)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6470..6490
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6587..7175
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6640..6660)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7058..7069)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7323..7503
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7550..7568)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7636..7740
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7741..8601
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7959..7978)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8772..131
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
       61 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      121 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      181 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      241 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      301 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      361 aatgcagcaa gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
      421 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
      481 aaagctcccc gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
      541 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
      601 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga
      661 ttacgaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
      721 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
      781 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
      841 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
      901 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
      961 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     1021 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     1081 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     1141 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     1201 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     1261 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     1321 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     1381 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     1441 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     1501 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     1561 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     1621 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1681 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1741 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1801 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1861 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1921 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1981 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     2041 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
     2101 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     2161 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     2221 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     2281 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     2341 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     2401 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     2461 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     2521 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     2581 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     2641 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2701 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2761 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2821 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2881 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     2941 gccgaattca caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     3001 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     3061 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     3121 agtttggact agtcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     3181 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     3241 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     3301 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     3361 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
     3421 ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt
     3481 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     3541 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     3601 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     3661 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     3721 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     3781 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     3841 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     3901 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     3961 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     4021 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     4081 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     4141 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     4201 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     4261 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgaagc
     4321 ggccgccatg ggcacacgac tccccaagaa gaggaaggtg tccaatttac tgaccgtaca
     4381 ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca agaacctgat
     4441 ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc ttctgtccgt
     4501 ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc ccgcagaacc
     4561 tgaagatgtt cgagattatc ttctatatct tcaggcgcgc ggtctggcag taaaaactat
     4621 ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc cacgaccaag
     4681 tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg ttgatgccgg
     4741 tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg ttcgttcact
     4801 catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg ggattgctta
     4861 taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata tctcacgtac
     4921 tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta gcaccgcagg
     4981 tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga tttccgtctc
     5041 tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa atggtgttgc
     5101 cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt ttgaagcaac
     5161 tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg cctggtctgg
     5221 acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt caataccgga
     5281 gatcatgcaa gctggtggct ggaccaatgt aaatattgtc atgaactata tccgtaacct
     5341 ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattaga gatccctccc
     5401 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     5461 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     5521 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     5581 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     5641 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     5701 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     5761 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     5821 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     5881 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     5941 tttgaaaaac acgatgataa tatggccaca catatgaccg agtacaagcc cacggtgcgc
     6001 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     6061 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     6121 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     6181 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     6241 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     6301 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     6361 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     6421 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     6481 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     6541 acctggtgca tgacccgcaa gcccggtgcc taaatcgata gatcctaatc aacctctgga
     6601 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     6661 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     6721 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     6781 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     6841 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     6901 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     6961 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     7021 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7081 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7141 gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt
     7201 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     7261 ttcactccca acgaagacaa gatcacctgc aggacaggcg cgccctgctt tttgcttgta
     7321 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7381 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7441 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7501 gcacccgggc gattaaggaa agggctagat cattcttgaa gacgaaaggg cctcgtgata
     7561 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     7621 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     7681 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     7741 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     7801 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     7861 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     7921 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     7981 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     8041 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     8101 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     8161 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     8221 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     8281 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     8341 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     8401 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     8461 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     8521 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     8581 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     8641 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     8701 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     8761 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     8821 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     8881 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     8941 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     9001 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     9061 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     9121 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     9181 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcg
//
