LOCUS       Exported                8608 bp ds-DNA     circular SYN 29-MAR-2022
DEFINITION  self-inactivating 3rd generation lentiviral plasmid for 
            co-expression of your gene of interest and Luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pHIV-Luciferase
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8608)
  TITLE     Lentivirus-EF1alpha-MCS-IRES-Luciferase
REFERENCE   2  (bases 1 to 8608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 29, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    3..555
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(147..164)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     374..393
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             562..2214
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2217..2233)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2218..2234)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2743..2923
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(2985..3573)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3065..3084)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3744..4604)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4367..4386
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4605..4709)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4784..4803)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4975..5354
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5356..5554
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5509..5529
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5572..5752
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5799..5924
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6421..6654
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6839..6883
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7181..7298
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7361..8539
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7592..8530
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8487..8507
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
       61 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
      121 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
      181 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
      241 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
      301 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
      361 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
      421 ggtaccccat tgtatgggat ctgatctggg gcctcggtac acatgcttta catgtgttta
      481 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
      541 aacacgatga taatatgggg gatggaagat gccaaaaaca ttaagaaggg cccagcgcca
      601 ttctacccac tcgaagacgg gaccgccggc gagcagctgc acaaagccat gaagcgctac
      661 gccctggtgc ccggcaccat cgcctttacc gacgcacata tcgaggtgga cattacctac
      721 gccgagtact tcgagatgag cgttcggctg gcagaagcta tgaagcgcta tgggctgaat
      781 acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc agttcttcat gcccgtgttg
      841 ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg acatctacaa cgagcgcgag
      901 ctgctgaaca gcatgggcat cagccagccc accgtcgtat tcgtgagcaa gaaagggctg
      961 caaaagatcc tcaacgtgca aaagaagcta ccgatcatac aaaagatcat catcatggat
     1021 agcaagaccg actaccaggg cttccaaagc atgtacacct tcgtgacttc ccatttgcca
     1081 cccggcttca acgagtacga cttcgtgccc gagagcttcg accgggacaa aaccatcgcc
     1141 ctgatcatga acagtagtgg cagtaccgga ttgcccaagg gcgtagccct accgcaccgc
     1201 accgcttgtg tccgattcag tcatgcccgc gaccccatct tcggcaacca gatcatcccc
     1261 gacaccgcta tcctcagcgt ggtgccattt caccacggct tcggcatgtt caccacgctg
     1321 ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga ggagctattc
     1381 ttgcgcagct tgcaagacta taagattcaa tctgccctgc tggtgcccac actatttagc
     1441 ttcttcgcta agagcactct catcgacaag tacgacctaa gcaacttgca cgagatcgcc
     1501 agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg cttccaccta
     1561 ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct gatcaccccc
     1621 gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga ggctaaggtg
     1681 gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct gtgcgtccgt
     1741 ggccccatga tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc tctcatcgac
     1801 aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga gcacttcttc
     1861 atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc cccagccgaa
     1921 ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc cggcctgccc
     1981 gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg taaaaccatg
     2041 accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa gaagctgcgc
     2101 ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc
     2161 aagatccgcg agattctcat taaggccaag aagggcggca agatcgccgt gtaaatcgat
     2221 accgtcgacc tcgatcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     2281 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     2341 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     2401 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc
     2461 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
     2521 gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     2581 caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc
     2641 ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta
     2701 gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac
     2761 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     2821 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     2881 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatgtg agcaaaaggc
     2941 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3001 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3061 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3121 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3181 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3241 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3301 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3361 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3421 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3481 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3541 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3601 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3661 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3721 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     3781 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     3841 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     3901 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     3961 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4021 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4081 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4141 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     4201 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     4261 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4321 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     4381 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     4441 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     4501 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     4561 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     4621 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     4681 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     4741 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
     4801 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     4861 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     4921 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
     4981 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     5041 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     5101 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     5161 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     5221 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     5281 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     5341 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     5401 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     5461 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     5521 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
     5581 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     5641 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     5701 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     5761 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     5821 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     5881 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     5941 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     6001 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     6061 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     6121 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     6181 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     6241 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
     6301 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     6361 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     6421 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     6481 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     6541 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     6601 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     6661 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     6721 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     6781 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     6841 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     6901 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     6961 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     7021 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     7081 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg
     7141 tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     7201 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     7261 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     7321 gagatccagt ttggttagta ccgggcccgc tctagcgtga ggctccggtg cccgtcagtg
     7381 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     7441 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     7501 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     7561 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     7621 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     7681 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     7741 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     7801 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     7861 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     7921 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     7981 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     8041 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     8101 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     8161 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     8221 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     8281 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     8341 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     8401 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     8461 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     8521 tccatttcag gtgtcgtgag cggccgctga actgaattca tcgacgttaa ctattctaga
     8581 gtacccgggc taggatccgc ccctctcc
//
