LOCUS       Exported                6507 bp ds-DNA     circular SYN 06-APR-2022
DEFINITION  Envelope protein for producing lentiviral and MuLV retroviral 
            particles. Use in conjunction with a packaging vector such as 
            pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral)..
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-VSV-G
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, 
            Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD
  TITLE     Lentivirus-delivered stable gene silencing by RNAi in primary cells.
  JOURNAL   RNA 2003 Apr;9(4):493-501.
  PUBMED    12649500
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 6, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(35..52)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(206..794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(286..305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(965..1825)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1588..1607
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1826..1930)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1956..2411)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2093..2114)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2305..2324
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2538..2560
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2552..2569
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2553..2569
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2579..2598
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2579..2597
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        2718..3097
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3098..3301
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3252..3272
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3298..3322
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          3413..3985
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             4071..5606
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     primer_bind     complement(5833..5852)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5898..5953
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5952..5971)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6311..6327)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6311..6327)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6324..6346)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6335..6351
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6359..6389)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6404..6425
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
       61 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      121 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      961 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1021 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1081 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1141 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1201 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1261 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1321 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1381 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1441 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1501 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1561 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1621 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1681 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1741 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1801 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1861 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1921 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     1981 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     2041 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     2101 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     2161 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     2221 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     2281 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     2341 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     2401 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     2461 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     2521 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta
     2581 atacgactca ctatagggcg aattggagct ccaccgcggt ggcggccgct ctagagagct
     2641 tggcccattg catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc
     2701 aacattaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
     2761 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     2821 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     2881 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     2941 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     3001 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     3061 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     3121 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     3181 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     3241 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     3301 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     3361 aagacaccgg gaccgatcca gcctccggtc gaccgatcct gagaacttca gggtgagttt
     3421 ggggaccctt gattgttctt tctttttcgc tattgtaaaa ttcatgttat atggaggggg
     3481 caaagttttc agggtgttgt ttagaatggg aagatgtccc ttgtatcacc atggaccctc
     3541 atgataattt tgtttctttc actttctact ctgttgacaa ccattgtctc ctcttatttt
     3601 cttttcattt tctgtaactt tttcgttaaa ctttagcttg catttgtaac gaatttttaa
     3661 attcactttt gtttatttgt cagattgtaa gtactttctc taatcacttt tttttcaagg
     3721 caatcagggt atattatatt gtacttcagc acagttttag agaacaattg ttataattaa
     3781 atgataaggt agaatatttc tgcatataaa ttctggctgg cgtggaaata ttcttattgg
     3841 tagaaacaac tacaccctgg tcatcatcct gcctttctct ttatggttac aatgatatac
     3901 actgtttgag atgaggataa aatactctga gtccaaaccg ggcccctctg ctaaccatgt
     3961 tcatgccttc ttctctttcc tacagctcct gggcaacgtg ctggttgttg tgctgtctca
     4021 tcattttggc aaagaattcc tcgacggatc cctcgaggaa ttctgacact atgaagtgcc
     4081 ttttgtactt agccttttta ttcattgggg tgaattgcaa gttcaccata gtttttccac
     4141 acaaccaaaa aggaaactgg aaaaatgttc cttctaatta ccattattgc ccgtcaagct
     4201 cagatttaaa ttggcataat gacttaatag gcacagcctt acaagtcaaa atgcccaaga
     4261 gtcacaaggc tattcaagca gacggttgga tgtgtcatgc ttccaaatgg gtcactactt
     4321 gtgatttccg ctggtatgga ccgaagtata taacacattc catccgatcc ttcactccat
     4381 ctgtagaaca atgcaaggaa agcattgaac aaacgaaaca aggaacttgg ctgaatccag
     4441 gcttccctcc tcaaagttgt ggatatgcaa ctgtgacgga tgccgaagca gtgattgtcc
     4501 aggtgactcc tcaccatgtg ctggttgatg aatacacagg agaatgggtt gattcacagt
     4561 tcatcaacgg aaaatgcagc aattacatat gccccactgt ccataactct acaacctggc
     4621 attctgacta taaggtcaaa gggctatgtg attctaacct catttccatg gacatcacct
     4681 tcttctcaga ggacggagag ctatcatccc tgggaaagga gggcacaggg ttcagaagta
     4741 actactttgc ttatgaaact ggaggcaagg cctgcaaaat gcaatactgc aagcattggg
     4801 gagtcagact cccatcaggt gtctggttcg agatggctga taaggatctc tttgctgcag
     4861 ccagattccc tgaatgccca gaagggtcaa gtatctctgc tccatctcag acctcagtgg
     4921 atgtaagtct aattcaggac gttgagagga tcttggatta ttccctctgc caagaaacct
     4981 ggagcaaaat cagagcgggt cttccaatct ctccagtgga tctcagctat cttgctccta
     5041 aaaacccagg aaccggtcct gctttcacca taatcaatgg taccctaaaa tactttgaga
     5101 ccagatacat cagagtcgat attgctgctc caatcctctc aagaatggtc ggaatgatca
     5161 gtggaactac cacagaaagg gaactgtggg atgactgggc accatatgaa gacgtggaaa
     5221 ttggacccaa tggagttctg aggaccagtt caggatataa gtttccttta tacatgattg
     5281 gacatggtat gttggactcc gatcttcatc ttagctcaaa ggctcaggtg ttcgaacatc
     5341 ctcacattca agacgctgct tcgcaacttc ctgatgatga gagtttattt tttggtgata
     5401 ctgggctatc caaaaatcca atcgagcttg tagaaggttg gttcagtagt tggaaaagct
     5461 ctattgcctc ttttttcttt atcatagggt taatcattgg actattcttg gttctccgag
     5521 ttggtatcca tctttgcatt aaattaaagc acaccaagaa aagacagatt tatacagaca
     5581 tagagatgaa ccgacttgga aagtaactca aatcctgcac aacagattct tcatgtttgg
     5641 accaaatcaa cttgtgatac catgctcaaa gaggcctcaa ttatatttga gtttttaatt
     5701 tttatgaaaa aaaaaaaaaa aaacggaatt cctcgaggga tccgtcgagg aattcactcc
     5761 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa
     5821 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     5881 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     5941 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     6001 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg
     6061 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
     6121 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
     6181 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
     6241 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacggat cggccgcaat
     6301 tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6361 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     6421 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     6481 attaatgaat cggccaacgc gcgggga
//
