LOCUS Exported 11716 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pTight-9-124 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11716) AUTHORS Yoo AS, Sun AX, Li L, Shcheglovitov A, Portmann T, Li Y, Lee-Messer C, Dolmetsch RE, Tsien RW, Crabtree GR TITLE MicroRNA-mediated conversion of human fibroblasts to neurons. JOURNAL Nature. 2011 Jul 13. doi: 10.1038/nature10323. PUBMED 21753754 REFERENCE 2 (bases 1 to 11716) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11716 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2063..2180 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" CDS complement(2245..2619) /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" regulatory 2616..2625 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" promoter complement(2629..2647) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter complement(2639..2686) /label=EM7 promoter /note="synthetic bacterial promoter " promoter 2763..3077 /label=tight TRE promoter /note="Tet-responsive promoter PTight, consisting of seven tet operator sequences followed by the minimal CMV promoter" protein_bind 2771..2789 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2807..2825 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2842..2860 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2878..2896 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2914..2932 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2949..2967 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2985..3003 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 3050..3074 /label=LNCX /note="Human CMV promoter, forward primer" CDS 3097..3792 /codon_start=1 /product="red fluorescent protein from Entacmaea quadricolor" /label=TurboRFP /note="mammalian codon-optimized" /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC DLPSKLGHR" misc_feature 4494..5066 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(4661..4678) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 4888..4907 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5069..5671 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind 5568..5588 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 5687..6275 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5740..5760) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6158..6169) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6482..6715 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(6816..6833) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6822..7046 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 7092..7520 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7179..7198) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7389..7410 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7529..7549) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7534..7863 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7714..7849 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7776..7795 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" polyA_signal 9071..9192 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9241..9257) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9241..9257) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9254..9276) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9265..9281 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9289..9319) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9334..9355 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9472..9489) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9643..10231) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9723..9742) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10402..11262) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11025..11044 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(11263..11367) /gene="bla" /label=AmpR promoter polyA_signal 11415..11549 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac 2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag 2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa 2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag 2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt 2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat 2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag 2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg 2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac 2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg 2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt 2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc 2701 cgaggttcta gagaccggtg gttaacgcgg atcccatcga tgaggccctt tcgtcttcac 2761 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat 2821 agagaacgat gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc 2881 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt 2941 cgagtttatc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag 3001 agaacgtatg tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag 3061 tgaaccgtca gatcgccagc gctaccggtc gccaccatga gcgagctgat caaggagaac 3121 atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc 3181 gagggcgaag gcaagcccta cgagggcacc cagaccatga agatcaaggt ggtcgagggc 3241 ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcaaagcc 3301 ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc tgagggcttc 3361 acatgggaga gaatcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc 3421 agcttccaga acggctgcat catctacaac gtcaagatca acggggtgaa cttcccatcc 3481 aacggccctg tgatgcagaa gaaaacacgc ggctgggagg ccaacaccga gatgctgtac 3541 cccgctgacg gcggcctgag aggccacagc cagatggccc tgaagctcgt gggcgggggc 3601 tacctgcact gctccttcaa gaccacatac agatccaaga aacccgctaa gaacctcaag 3661 atgcccggct tccacttcgt ggaccacaga ctggaaagaa tcaaggaggc cgacaaagag 3721 acctacgtcg agcagcacga gatggctgtg gccaagtact gcgacctccc tagcaaactg 3781 gggcacagat aactcgagcg gacgcgtcgg cggcctgtcg gtcccctctg gctctccgcg 3841 tgcgcccggg gctgcgggag cggagtccgc tcaggccccc gcgccgggtc ggcggcggca 3901 cgtggggccc acagcgcggc agcggtgccg gcgaatggga ggcccgtttc tctctttggt 3961 tatctagctg tatgagtgcc acagagccgt cataaagcta gataaccgaa agtagaaatg 4021 actctcacaa cttctgcgtg cgatggcccg gccggccgcc ctggccacag gagccagagg 4081 agcccagaga agcccacgcc acccagggat tccagctcga cggagcggcc acgccgggac 4141 caggtatccg acctcggctc tccttctagt aggtgggagt actgctcaga gctacaactc 4201 taggagtagg gactccaagc ctagagctcc aagagagggt gaagggcagg gagaaaatta 4261 tagtaatagt tgcaatgagt cacttgcttc tagatcaaga tcagagactc tgctctccgt 4321 gttcacagcg gaccttgatt taatgtcata caattaaggc acgcggtgaa tgccaagagc 4381 ggagcctaca gctgcacttg aaggacatcc gagagaagtt aggaagggtg gggagaaaca 4441 attctagaat gaacccatcc tgtgcgacac ggccgcaaat tccgcaaatt ccgcccctct 4501 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt 4561 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct 4621 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa 4681 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg 4741 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc 4801 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg 4861 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg 4921 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc 4981 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg 5041 gttttccttt gaaaaacacg ataataccat ggccaccgag tacaagccca cggtgcgcct 5101 cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta 5161 ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca 5221 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg 5281 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga 5341 gatcggctcg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga 5401 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt 5461 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc 5521 ggccgagcgc gctggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt 5581 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac 5641 ctggtgcatg acccgcaagc ccggtgcctg agttcgcgtc tggaacaatc aacctctgga 5701 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 5761 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 5821 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 5881 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 5941 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 6001 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 6061 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 6121 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 6181 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 6241 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac 6301 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc 6361 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta 6421 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga 6481 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct 6541 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 6601 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 6661 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 6721 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 6781 gagaggcctt gacattgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca 6841 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 6901 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 6961 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 7021 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag 7081 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 7141 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 7201 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 7261 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 7321 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 7381 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 7441 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 7501 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc 7561 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 7621 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 7681 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 7741 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 7801 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 7861 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gatgaaaaag 7921 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc 7981 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg 8041 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt 8101 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaatta 8161 attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga 8221 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat 8281 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg 8341 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg 8401 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat 8461 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa 8521 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt 8581 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat 8641 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct 8701 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa 8761 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg 8821 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt 8881 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata 8941 gcacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat 9001 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt 9061 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 9121 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 9181 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg 9241 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 9301 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 9361 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 9421 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 9481 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 9541 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 9601 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 9661 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 9721 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 9781 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 9841 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 9901 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 9961 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 10021 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 10081 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 10141 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 10201 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 10261 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 10321 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 10381 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 10441 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 10501 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 10561 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 10621 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 10681 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 10741 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 10801 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 10861 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 10921 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 10981 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 11041 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 11101 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 11161 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 11221 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 11281 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 11341 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga 11401 cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 11461 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 11521 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc 11581 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct 11641 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta 11701 ctacaaactt agtagt //