LOCUS Exported 11591 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pTight-hMYT1L-N174 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11591) AUTHORS Yoo AS, Sun AX, Li L, Shcheglovitov A, Portmann T, Li Y, Lee-Messer C, Dolmetsch RE, Tsien RW, Crabtree GR TITLE MicroRNA-mediated conversion of human fibroblasts to neurons. JOURNAL Nature. 2011 Jul 13. doi: 10.1038/nature10323. PUBMED 21753754 REFERENCE 2 (bases 1 to 11591) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11591 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2028..2143 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" promoter 2235..2549 /label=tight TRE promoter /note="Tet-responsive promoter PTight, consisting of seven tet operator sequences followed by the minimal CMV promoter" protein_bind 2243..2261 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2279..2297 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2314..2332 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2350..2368 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2386..2404 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2421..2439 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2457..2475 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 2522..2546 /label=LNCX /note="Human CMV promoter, forward primer" promoter 6169..6668 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(6204..6227) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 6601..6620 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 6695..7489 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(6749..6768) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 7359..7378 /label=Neo-F /note="Neomycin resistance gene, forward primer" misc_feature 7513..8101 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7566..7586) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7984..7995) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 8309..8942 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" primer_bind complement(9071..9087) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9071..9087) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9084..9106) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9095..9111 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9119..9149) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9164..9185 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9302..9319) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9473..10058) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9553..9572) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10229..11089) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10852..10871 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(11090..11194) /gene="bla" /label=AmpR promoter polyA_signal 11242..11376 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(11279..11298) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 11333..11352 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaatc gatgaggccc 2221 tttcgtcttc actcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc 2281 cctatcagtg atagagaacg atgtcgagtt tactccctat cagtgataga gaacgtatgt 2341 cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata 2401 gagaacgtat gtcgagttta tccctatcag tgatagagaa cgtatgtcga gtttactccc 2461 tatcagtgat agagaacgta tgtcgaggta ggcgtgtacg gtgggaggcc tatataagca 2521 gagctcgttt agtgaaccgt cagatcgcct ggagaaggat ccgcggccgc gccggctcta 2581 gatcgcgaac gcgtgaattc accatggagg tggacaccga ggagaagcgg catcgcacgc 2641 ggtccaaagg ggttcgagtt cccgtggaac cagccataca agagctgttc agctgtccca 2701 cccctggctg tgacggcagt ggtcatgtca gtggcaaata tgcaagacac agaagtgtat 2761 atggttgtcc cttggcgaaa aaaagaaaaa cacaagataa acagccccag gaacctgctc 2821 ctaaacgaaa gccatttgcc gtgaaagcag acagctcctc agtggatgag tgtgacgaca 2881 gtgatgggac tgaggacatg gatgagaagg aggaggatga gggggaggag tactccgagg 2941 acaatgacga gccaggggat gaggacgagg aggacgagga gggggaccgg gaggaggagg 3001 aggagatcga ggaggaggat gaggacgatg acgaggatgg agaagatgtg gaggatgaag 3061 aagaggaaga ggaggaggag gaggaggagg aagaggaaga agaaaacgaa gaccatcaaa 3121 tgaattgtca caatactcga ataatgcaag acacagaaaa ggatgataac aataatgacg 3181 aatatgacaa ttacgatgaa ctggtggcca agtcattgtt aaacctcggc aaaatcgctg 3241 aggatgcagc ctaccgggcc aggactgagt cagaaatgaa cagcaatacc tccaatagtc 3301 tggaagacga tagtgacaaa aacgaaaacc tgggtcggaa aagtgagttg agtttagact 3361 tagacagtga tgttgttaga gaaacagtgg actcccttaa actattagcc caaggacacg 3421 gtgttgtgct ctcagaaaac atgaatgaca gaaattatgc agacagcatg tcgcagcaag 3481 acagtagaaa tatgaattac gtcatgttgg ggaagcccat gaacaatgga ctcatggaaa 3541 agatggtgga ggagagcgat gaggaggtgt gtctgagcag tctggagtgt ttgaggaatc 3601 agtgcttcga cctggccagg aagctcagtg agaccaaccc gcaggagagg aatccgcagc 3661 agaacatgaa catccgtcag catgtccggc cagaagagga cttcccagga aggacgccgg 3721 acagaaacta ctcggacatg ctgaacctca tgcggctgga ggagcagttg agcccccggt 3781 cgagagtgtt tgccagctgt gcgaaggagg atgggtgtca tgagcgggac gacgatacca 3841 cctctgtgaa ctcggacagg tctgaagagg tgttcgacat gaccaagggg aacctgaccc 3901 tgctggagaa agccatcgct ttggaaacgg aaagagcaaa ggccatgagg gagaagatgg 3961 ccatggaagc tgggaggagg gacaatatga ggtcatatga ggaccagtct ccgagacaac 4021 ttcccgggga ggacagaaag cctaaatcca gtgacagcca tgtcaaaaag ccatactatg 4081 atccctcaag aacagaaaag aaagagagca agtgtccaac ccccgggtgt gatggaaccg 4141 gccacgtaac tgggctgtac ccacatcacc gcagcctgtc cggatgcccg cacaaagata 4201 gggtccctcc agaaatcctt gccatgcatg aaagtgtcct caagtgcccc actccgggct 4261 gcacggggcg cgggcatgtc aacagcaaca ggaactccca ccgaagcctc tccggatgcc 4321 cgatcgctgc agcagagaaa ctggccaagg cacaggaaaa gcaccagagc tgcgacgtgt 4381 ccaagtccag ccaggcctcg gaccgcgtgc tcaggccaat gtgctttgtg aagcagctgg 4441 agattcctca gtatggctac agaaacaatg tccccacaac tacgccgcgt tccaacctgg 4501 ccaaggagct cgagaaatat tccaagacct cgtttgaata caacagttac gacaaccata 4561 cttatggcaa gcgagccata gctcccaagg tgcaaaccag ggatatatcc cccaaaggat 4621 atgatgatgc gaagcggtac tgcaaggacc ccagccccag cagcagcagc accagcagct 4681 acgcgcccag cagcagcagc aacctgagct gcggcggggg cagcagcgcc agcagcacgt 4741 gcagcaagag cagcttcgac tacacgcacg acatggaggc ggcccacatg gcggccaccg 4801 ccatcctcaa cctgtccacg cgctgccgcg agatgccgca gaacctgagc accaagccgc 4861 aggacctgtg cgccacgcgg aaccctgaca tggaggtgga tgagaacggg accctggacc 4921 tcagcatgaa caagcagagg ccgcgggaca gctgctgccc catcctgacc cctctggagc 4981 ccatgtcccc ccagcagcag gcagtgatga acaaccggtg tttccagctg ggcgagggcg 5041 actgctggga cttgcccgta gactacacca aaatgaaacc ccggaggata gacgaggacg 5101 agtccaaaga cattactcca gaagacttgg acccattcca ggaggctcta gaagaaagac 5161 ggtatcccgg ggaggtgacc atcccaagtc ccaaacccaa gtaccctcag tgcaaggaga 5221 gcaaaaagga cttaataact ctgtctggct gccccctggc ggacaaaagc attcgaagta 5281 tgctggccac cagctcccaa gaactcaagt gccccacgcc tggctgtgat ggttctggac 5341 atatcaccgg caattatgct tctcatcgga gcctttcagg ttgcccaaga gcaaagaaaa 5401 gtggtatcag gatagcacag agcaaagaag ataaagaaga tcaagaaccc atcaggtgtc 5461 cggtccccgg gtgcgacggc cagggccaca tcactgggaa gtacgcgtcc catcgcagcg 5521 cctccgggtg ccccttggcg gccaagaggc agaaagacgg gtacctgaat ggctcccagt 5581 tctcctggaa gtcggtcaag acggaaggca tgtcctgccc cacgccagga tgcgacggct 5641 caggccacgt cagcggcagc ttcctcacac accgcagctt gtcaggatgc ccgagagcca 5701 cgtcagcgat gaagaaggca aagctttctg gagagcagat gctgaccatc aaacagcggg 5761 ccagcaacgg tatagaaaat gatgaagaaa tcaaacagtt agatgaagaa atcaaggagc 5821 taaatgaatc caattcccag atggaagccg atatgattaa actcagaact cagattacca 5881 cgatggagag caacctgaag accatcgaag aggagaacaa agtgattgag cagcagaacg 5941 agtctctcct ccacgagctg gcgaacctga gccagtctct gatccacagc ctggctaaca 6001 tccagctgcc gcacatggat ccaatcaatg aacaaaattt tgatgcttac gtgactactt 6061 tgacggaaat gtatacaaat caagatcgtt atcagagtcc agaaaataaa gccctactgg 6121 aaaatataaa gcaggctgtg agaggaattc aggtctgaga attctaccgg gtaggggagg 6181 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct 6241 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc 6301 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc 6361 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc 6421 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc 6481 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg 6541 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg 6601 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg 6661 gcctttcgac ctgcagccca agcttcctag gaccatgatt gaacaagatg gattgcacgc 6721 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 6781 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 6841 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg 6901 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 6961 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 7021 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 7081 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 7141 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 7201 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg 7261 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 7321 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 7381 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 7441 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgac ccgggcgggg 7501 cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 7561 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 7621 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 7681 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 7741 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 7801 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 7861 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt 7921 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 7981 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 8041 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg 8101 cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata 8161 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt 8221 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 8281 agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa 8341 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac 8401 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca 8461 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct 8521 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc 8581 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga 8641 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc 8701 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac 8761 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 8821 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 8881 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 8941 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 9001 agagagtgag aggccttgac attgctagcg ttttaccgtc gacctctagc tagagcttgg 9061 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 9121 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 9181 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 9241 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 9301 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 9361 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 9421 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 9481 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 9541 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 9601 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 9661 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 9721 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 9781 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 9841 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 9901 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 9961 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt 10021 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 10081 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 10141 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 10201 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 10261 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 10321 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 10381 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 10441 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 10501 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 10561 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 10621 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 10681 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 10741 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 10801 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 10861 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 10921 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 10981 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 11041 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 11101 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 11161 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 11221 acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta caaataaagc 11281 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 11341 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa 11401 aatcatccca aacttcccac cccataccct attaccactg ccaattacct agtggtttca 11461 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta 11521 aatatgtact acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca 11581 aacttagtag t //