LOCUS       Exported                9989 bp ds-DNA     circular SYN 27-APR-2022
DEFINITION  Express CD74-ROS1 fusion (C6;R34) harboring ROS1 L2000V mutation in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pLenti-CD74-ROS1 L2000V
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Lin JJ, Choudhury NJ, Yoda S, Zhu VW, Johnson TW, Sakhtemani R, 
            Dagogo-Jack I, Digumarthy SR, Lee C, Do A, Peterson J, 
            Prutisto-Chang K, Malik W, Hubbeling HG, Langenbucher A, Schoenfeld 
            AJ, Falcon CJ, Temel JS, Sequist LV, Yeap BY, Lennerz JK, Shaw AT, 
            Lawrence MS, Ou SI, Hata AN, Drilon A, Gainor JF
  TITLE     Spectrum of Mechanisms of Resistance to Crizotinib and Lorlatinib in
            ROS1 Fusion-Positive Lung Cancer.
  JOURNAL   Clin Cancer Res. 2021 May 15;27(10):2899-2909. doi: 
            10.1158/1078-0432.CCR-21-0032. Epub 2021 Mar 8.
  PUBMED    33685866
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 27, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9989
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        69..272
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     223..243
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     269..293
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     368..387
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        368..386
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    416..440
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2589..2613)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2645..2668
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2684..2710
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2733..3285
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2877..2894)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3104..3123
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3297..3896
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3297..3316)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3793..3813
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3913..4501
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3966..3986)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4384..4395)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4576..4809
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4928..4946)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5014..5118
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5119..5979
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5337..5356)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6150..6738
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6639..6658
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6892..6909
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     7001..7020
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    7149..7170
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7185..7215
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7223..7239
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7228..7250
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7247..7263
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7247..7263
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7313..7332
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7524..8157
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8204..8329
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8826..9059
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9244..9288
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9585..9702
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        9754..68
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
       61 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      121 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      181 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      241 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
      301 gacgccatcc acgctgtttt gacctccata gaagacaccg gcggccgcag atctctcgag
      361 gttgatctaa tacgactcac tatagggaga cccaagctgg ctagttaagc tatcaacaag
      421 tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc atgcacagga ggagaagcag
      481 gagctgtcgg gaagatcaga agccagtcat ggatgaccag cgcgacctta tctccaacaa
      541 tgagcaactg cccatgctgg gccggcgccc tggggccccg gagagcaagt gcagccgcgg
      601 agccctgtac acaggctttt ccatcctggt gactctgctc ctcgctggcc aggccaccac
      661 cgcctacttc ctgtaccagc agcagggccg gctggacaaa ctgacagtca cctcccagaa
      721 cctgcagctg gagaacctgc gcatgaagct tcccaagcct cccaagcctg tgagcaagat
      781 gcgcatggcc accccgctgc tgatgcaggc gctgcccatg ggagccctgc cccaggggcc
      841 catgcagaat gccaccaagt atggcaacat gacagaggac catgtgatgc acctgctcca
      901 gaatgctgac cccctgaagg tgtacccgcc actgaagggg agcttcccgg agaacctgag
      961 acaccttaag aacaccatgg agaccataga ctggaaggtc tttgagagct ggatgcacca
     1021 ttggctcctg tttgaaatga gcaggcactc cttggagcaa aagcccactg acgctccacc
     1081 gaaagatgat ttttggatac cagaaacaag tttcatactt actattatag ttggaatatt
     1141 tctggttgtt acaatcccac tgacctttgt ctggcataga agattaaaga atcaaaaaag
     1201 tgccaaggaa ggggtgacag tgcttataaa cgaagacaaa gagttggctg agctgcgagg
     1261 tctggcagcc ggagtaggcc tggctaatgc ctgctatgca atacatactc ttccaaccca
     1321 agaggagatt gaaaatcttc ctgccttccc tcgggaaaaa ctgactctgc gtctcttgct
     1381 gggaagtgga gcctttggag aagtgtatga aggaacagca gtggacatct taggagttgg
     1441 aagtggagaa atcaaagtag cagtgaagac tttgaagaag ggttccacag accaggagaa
     1501 gattgaattc ctgaaggagg cacatgtgat gagcaaattt aatcatccca acattctgaa
     1561 gcagcttgga gtttgtctgc tgaatgaacc ccaatacatt atcctggaac tgatggaggg
     1621 aggagacctt cttacttatt tgcgtaaagc ccggatggca acgttttatg gtcctttact
     1681 caccttggtt gaccttgtag acctgtgtgt agatatttca aaaggctgtg tctacttgga
     1741 acggatgcat ttcattcaca gggatctggc agctagaaat tgccttgttt ccgtgaaaga
     1801 ctataccagt ccacggatag tgaagattgg agactttgga ctcgccagag acatctataa
     1861 aaatgattac tatagaaaga gaggggaagg cctgctccca gttcggtgga tggctccaga
     1921 aagtttgatg gatggaatct tcactactca atctgatgta tggtcttttg gaattctgat
     1981 ttgggagatt ttaactcttg gtcatcagcc ttatccagct cattccaacc ttgatgtgtt
     2041 aaactatgtg caaacaggag ggagactgga gccaccaaga aattgtcctg atgatctgtg
     2101 gaatttaatg acccagtgct gggctcaaga acccgaccaa agacctactt ttcatagaat
     2161 tcaggaccaa cttcagttat tcagaaattt tttcttaaat agcatttata agtccagaga
     2221 tgaagcaaac aacagtggag tcataaatga aagctttgaa ggtgaagatg gcgatgtgat
     2281 ttgtttgaat tcagatgaca ttatgccagt tgctttaatg gaaacgaaga accgagaagg
     2341 gttaaactat atggtacttg ctacagaatg tggccaaggt gaagaaaagt ctgagggtcc
     2401 tctaggctcc caggaatctg aatcttgtgg tctgaggaaa gaagagaagg aaccacatgc
     2461 agacaaagat ttctgccaag aaaaacaagt ggcttactgc ccttctggca agcctgaagg
     2521 cctgaactat gcctgtctca ctcacagtgg atatggagat gggtctgatt aaaagggtgg
     2581 gcgcgccgac ccagctttct tgtacaaagt ggtttagcta gagggcccgc ggttcgaact
     2641 cgaggactac aaggacgacg atgacaagtc ggccgctgga ggatacccct acgacgtgcc
     2701 cgactacgcc tagaattccg cccccccccc taacgttact ggccgaagcc gcttggaata
     2761 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     2821 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     2881 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     2941 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     3001 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     3061 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     3121 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     3181 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     3241 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatga
     3301 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     3361 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     3421 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     3481 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     3541 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     3601 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     3661 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     3721 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     3781 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     3841 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgatgca
     3901 tcgatggatc ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     3961 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     4021 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     4081 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     4141 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     4201 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     4261 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     4321 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     4381 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     4441 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     4501 cctgagatcc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     4561 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     4621 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     4681 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     4741 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4801 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     4861 gaatatcaga gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga
     4921 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     4981 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5041 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5101 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5161 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5221 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5281 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5341 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     5401 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5461 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5521 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5581 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5641 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5701 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5761 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     5821 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     5881 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     5941 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6001 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6061 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6121 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6181 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6241 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6301 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6361 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6421 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6481 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6541 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6601 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6661 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6721 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6781 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6841 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     6901 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     6961 attcattaat gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg
     7021 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     7081 tttgcaaaaa gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     7141 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     7201 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     7261 gacatgatta cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7321 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
     7381 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
     7441 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
     7501 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
     7561 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
     7621 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     7681 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     7741 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
     7801 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     7861 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     7921 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     7981 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     8041 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8101 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     8161 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     8221 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     8281 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     8341 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     8401 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     8461 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     8521 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     8581 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     8641 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
     8701 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     8761 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     8821 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     8881 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     8941 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     9001 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     9061 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     9121 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     9181 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     9241 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     9301 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     9361 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     9421 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     9481 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     9541 cggtatcgcc gaattaattc acaaatggca gtattcatcc acaattttaa aagaaaaggg
     9601 gggattgggg ggtacagtgc agggaaaaga atagtagaca taatagcaac agacatacaa
     9661 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     9721 agcagagatc cagtttggac tagtggagtt ccgcgttaca taacttacgg taaatggccc
     9781 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     9841 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     9901 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     9961 cggtaaatgg cccgcctggc attatgccc
//
