LOCUS Exported 4723 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pPAmCherry1-N1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4723) AUTHORS Subach FV, Patterson GH, Manley S, Gillette JM, Lippincott-Schwartz J, Verkhusha VV TITLE Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. JOURNAL Nat Methods. 2009 Feb;6(2):153-9. Epub 2009 Jan 25. PUBMED 19169259 REFERENCE 2 (bases 1 to 4723) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4723 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 519..539 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" misc_feature 591..671 /label=MCS /note="multiple cloning site" regulatory 673..682 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 679..1389 /codon_start=1 /product="photoactivatable monomeric derivative of DsRed fluorescent protein (Subach et al., 2009)" /label=PAmCherry1 /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(824..842) /label=mCherry-R /note="mCherry, reverse primer" polyA_signal 1511..1632 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1548..1567) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1602..1621 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1639..2094) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1776..1797) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1988..2007 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2121..2225 /gene="bla" /label=AmpR promoter promoter 2227..2584 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2250..2270) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2435..2570 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2497..2516 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2619..3413 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2673..2692) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3283..3302 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3601..3620) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3645..3692 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4021..4609 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4510..4529 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 661 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcattaag 721 gagttcatgc gcttcaaggt gcacatggag gggtccgtga acggccacgt gttcgagatc 781 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc 841 aagggcggcc ccctgccctt cacctgggac atcctgagcc ctcagttcat gtacggctcc 901 aatgcctacg tgaagcaccc cgccgacatc cccgactact ttaagctgtc cttccccgag 961 ggcttcaagt gggagcgcgt gatgaaattc gaggacggcg gcgtggtgac cgtgacccag 1021 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc 1081 ccctccgacg gccccgtgat gcagaagaag accatgggct gggaggccct ctccgagcgg 1141 atgtaccccg aggacggcgc cctgaagggc gaggtcaagc ccagagtgaa gctgaaggac 1201 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg 1261 cccggcgcct acaacgtcaa ccgcaagctg gacatcacca gccacaacga ggactacacc 1321 atcgtggagc agtacgagag agccgagggc cgccactcca ccggcggcat ggacgagctg 1381 tacaagtaag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt 1441 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat 1501 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 1561 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 1621 caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt 1681 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat 1741 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat 1801 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac 1861 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc 1921 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga 1981 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca 2041 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg 2101 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 2161 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 2221 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc 2281 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 2341 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 2401 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 2461 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 2521 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 2581 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 2641 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 2701 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 2761 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat 2821 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 2881 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 2941 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 3001 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 3061 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 3121 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc 3181 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 3241 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 3301 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 3361 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 3421 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 3481 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 3541 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac 3601 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca 3661 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg 3721 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt 3781 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc 3841 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca 3901 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 3961 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 4021 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 4081 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 4141 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt 4201 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 4261 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 4321 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 4381 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 4441 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 4501 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 4561 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 4621 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 4681 gttatcccct gattctgtgg ataaccgtat taccgccatg cat //