LOCUS Exported 9989 bp ds-DNA circular SYN 28-APR-2022 DEFINITION Express CD74-ROS1 fusion (C6;R34) harboring ROS1 L2086F mutation in mammalian cells. ACCESSION . VERSION . KEYWORDS pLenti-CD74-ROS1 L2086F SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9989) AUTHORS Lin JJ, Choudhury NJ, Yoda S, Zhu VW, Johnson TW, Sakhtemani R, Dagogo-Jack I, Digumarthy SR, Lee C, Do A, Peterson J, Prutisto-Chang K, Malik W, Hubbeling HG, Langenbucher A, Schoenfeld AJ, Falcon CJ, Temel JS, Sequist LV, Yeap BY, Lennerz JK, Shaw AT, Lawrence MS, Ou SI, Hata AN, Drilon A, Gainor JF TITLE Spectrum of Mechanisms of Resistance to Crizotinib and Lorlatinib in ROS1 Fusion-Positive Lung Cancer. JOURNAL Clin Cancer Res. 2021 May 15;27(10):2899-2909. doi: 10.1158/1078-0432.CCR-21-0032. Epub 2021 Mar 8. PUBMED 33685866 REFERENCE 2 (bases 1 to 9989) AUTHORS . TITLE Direct Submission JOURNAL Exported Apr 28, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9989 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 142..345 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 296..316 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 342..366 /label=LNCX /note="Human CMV promoter, forward primer" primer_bind 441..460 /label=T7 /note="T7 promoter, forward primer" promoter 441..459 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 489..513 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(2662..2686) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 2718..2741 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 2757..2783 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" misc_feature 2806..3358 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(2950..2967) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 3177..3196 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 3370..3969 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(3370..3389) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 3866..3886 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 3986..4574 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4039..4059) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4457..4468) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 4649..4882 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(5001..5019) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 5087..5191 /gene="bla" /label=AmpR promoter CDS 5192..6052 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5410..5429) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6223..6811 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6712..6731 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6965..6982 /label=L4440 /note="L4440 vector, forward primer" primer_bind 7074..7093 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 7222..7243 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7258..7288 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7296..7312 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7301..7323 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 7320..7336 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 7320..7336 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 7386..7405 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 7597..8230 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 8277..8402 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 8899..9132 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 9317..9361 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 9658..9775 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 9827..141 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc 61 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta 121 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag 181 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt 241 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa 301 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt 361 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccggcggccg 421 cagatctctc gaggttgatc taatacgact cactataggg agacccaagc tggctagtta 481 agctatcaac aagtttgtac aaaaaagcag gctccgcggc cgcccccttc accatgcaca 541 ggaggagaag caggagctgt cgggaagatc agaagccagt catggatgac cagcgcgacc 601 ttatctccaa caatgagcaa ctgcccatgc tgggccggcg ccctggggcc ccggagagca 661 agtgcagccg cggagccctg tacacaggct tttccatcct ggtgactctg ctcctcgctg 721 gccaggccac caccgcctac ttcctgtacc agcagcaggg ccggctggac aaactgacag 781 tcacctccca gaacctgcag ctggagaacc tgcgcatgaa gcttcccaag cctcccaagc 841 ctgtgagcaa gatgcgcatg gccaccccgc tgctgatgca ggcgctgccc atgggagccc 901 tgccccaggg gcccatgcag aatgccacca agtatggcaa catgacagag gaccatgtga 961 tgcacctgct ccagaatgct gaccccctga aggtgtaccc gccactgaag gggagcttcc 1021 cggagaacct gagacacctt aagaacacca tggagaccat agactggaag gtctttgaga 1081 gctggatgca ccattggctc ctgtttgaaa tgagcaggca ctccttggag caaaagccca 1141 ctgacgctcc accgaaagat gatttttgga taccagaaac aagtttcata cttactatta 1201 tagttggaat atttctggtt gttacaatcc cactgacctt tgtctggcat agaagattaa 1261 agaatcaaaa aagtgccaag gaaggggtga cagtgcttat aaacgaagac aaagagttgg 1321 ctgagctgcg aggtctggca gccggagtag gcctggctaa tgcctgctat gcaatacata 1381 ctcttccaac ccaagaggag attgaaaatc ttcctgcctt ccctcgggaa aaactgactc 1441 tgcgtctctt gctgggaagt ggagcctttg gagaagtgta tgaaggaaca gcagtggaca 1501 tcttaggagt tggaagtgga gaaatcaaag tagcagtgaa gactttgaag aagggttcca 1561 cagaccagga gaagattgaa ttcctgaagg aggcacatct gatgagcaaa tttaatcatc 1621 ccaacattct gaagcagctt ggagtttgtc tgctgaatga accccaatac attatcctgg 1681 aactgatgga gggaggagac cttcttactt atttgcgtaa agcccggatg gcaacgtttt 1741 atggtccttt actcaccttg gttgaccttg tagacctgtg tgtagatatt tcaaaaggct 1801 gtgtctactt ggaacggatg catttcattc acagggatct ggcagctaga aattgctttg 1861 tttccgtgaa agactatacc agtccacgga tagtgaagat tggagacttt ggactcgcca 1921 gagacatcta taaaaatgat tactatagaa agagagggga aggcctgctc ccagttcggt 1981 ggatggctcc agaaagtttg atggatggaa tcttcactac tcaatctgat gtatggtctt 2041 ttggaattct gatttgggag attttaactc ttggtcatca gccttatcca gctcattcca 2101 accttgatgt gttaaactat gtgcaaacag gagggagact ggagccacca agaaattgtc 2161 ctgatgatct gtggaattta atgacccagt gctgggctca agaacccgac caaagaccta 2221 cttttcatag aattcaggac caacttcagt tattcagaaa ttttttctta aatagcattt 2281 ataagtccag agatgaagca aacaacagtg gagtcataaa tgaaagcttt gaaggtgaag 2341 atggcgatgt gatttgtttg aattcagatg acattatgcc agttgcttta atggaaacga 2401 agaaccgaga agggttaaac tatatggtac ttgctacaga atgtggccaa ggtgaagaaa 2461 agtctgaggg tcctctaggc tcccaggaat ctgaatcttg tggtctgagg aaagaagaga 2521 aggaaccaca tgcagacaaa gatttctgcc aagaaaaaca agtggcttac tgcccttctg 2581 gcaagcctga aggcctgaac tatgcctgtc tcactcacag tggatatgga gatgggtctg 2641 attaaaaggg tgggcgcgcc gacccagctt tcttgtacaa agtggtttag ctagagggcc 2701 cgcggttcga actcgaggac tacaaggacg acgatgacaa gtcggccgct ggaggatacc 2761 cctacgacgt gcccgactac gcctagaatt ccgccccccc ccctaacgtt actggccgaa 2821 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt 2881 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg 2941 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc 3001 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc 3061 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa 3121 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc 3181 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg 3241 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac 3301 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg 3361 gccacaacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc 3421 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc 3481 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc 3541 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc 3601 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag 3661 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg 3721 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag 3781 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc 3841 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc 3901 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc 3961 ggtgcctgat gcatcgatgg atcctaatca acctctggat tacaaaattt gtgaaagatt 4021 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc 4081 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg 4141 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac 4201 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc 4261 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc 4321 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa 4381 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc 4441 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc 4501 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg 4561 ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt acaaggcagc tgtagatctt 4621 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa 4681 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 4741 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 4801 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 4861 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa 4921 cttgcaaaga aatgaatatc agagagtgag aggcccgggt taattaagga aagggctaga 4981 tcattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat 5041 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat 5101 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 5161 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 5221 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 5281 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 5341 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 5401 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg 5461 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 5521 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 5581 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 5641 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 5701 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 5761 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 5821 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 5881 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 5941 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 6001 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 6061 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 6121 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 6181 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 6241 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 6301 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 6361 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 6421 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 6481 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 6541 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 6601 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 6661 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 6721 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 6781 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 6841 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 6901 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 6961 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 7021 cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct gactaatttt ttttatttat 7081 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 7141 ggaggcctag gcttttgcaa aaagctcccc gtggcacgac aggtttcccg actggaaagc 7201 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt 7261 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac 7321 aggaaacagc tatgacatga ttacgaattt cacaaataaa gcattttttt cactgcattc 7381 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac 7441 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta 7501 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa 7561 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa 7621 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc 7681 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc 7741 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt 7801 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt 7861 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga 7921 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg 7981 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt 8041 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta 8101 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc 8161 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa 8221 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct 8281 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg 8341 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg 8401 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg 8461 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg 8521 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac 8581 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc 8641 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag 8701 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag 8761 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt 8821 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga 8881 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac 8941 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt 9001 gcagcagcag aacaatttgc tgatggctat tgaggcgcaa cagcatctgt tgcaactcac 9061 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga 9121 tcaacagctc ctggggattt tgggttgctc tggaaaactc atttgcacca ctgctgtgcc 9181 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat 9241 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc 9301 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt 9361 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt 9421 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag 9481 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag 9541 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt 9601 gaacggatct cgacggtatc gccgaattaa ttcacaaatg gcagtattca tccacaattt 9661 taaaagaaaa ggggggattg gggggtacag tgcagggaaa agaatagtag acataatagc 9721 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt 9781 ttattacagg gacagcagag atccagtttg gactagtgga gttccgcgtt acataactta 9841 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga 9901 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt 9961 tacggtaaac tgcccacttg gcagtacat //