LOCUS Exported 6886 bp ds-DNA circular SYN 06-MAY-2022 DEFINITION Expresses red to green photoconvertible Rab11a. ACCESSION . VERSION . KEYWORDS Dendra-Rab11a SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6886) AUTHORS Krishnan N, Swoger M, Rathbun LI, Fioramonti PJ, Freshour J, Bates M, Patteson AE, Hehnly H TITLE Rab11 endosomes and Pericentrin coordinate centrosome movement during pre-abscission in vivo. JOURNAL Life Sci Alliance. 2022 Mar 18;5(7). pii: 5/7/e202201362. doi: 10.26508/lsa.202201362. Print 2022 Jul. PUBMED 35304423 REFERENCE 2 (bases 1 to 6886) AUTHORS . TITLE Direct Submission JOURNAL Exported May 6, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6886 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 629..648 /label=T7 /note="T7 promoter, forward primer" promoter 629..647 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 677..686 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" misc_feature 1373..1438 /label=MCS /note="multiple cloning site" CDS 2137..2166 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 2182..2199 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(2243..2260) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 2249..2473 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 2519..2947 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2606..2625) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2816..2837 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(2956..2976) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 2961..3290 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3141..3276 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3203..3222 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3357..4151 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3411..3430) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4021..4040 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 4325..4446 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4362..4381) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4416..4435 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(4495..4511) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4495..4511) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4508..4530) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4519..4535 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4543..4573) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4588..4609 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4726..4743) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4897..5485) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4977..4996) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5656..6516) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6279..6298 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6517..6621) /gene="bla" /label=AmpR promoter primer_bind complement(6696..6715) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca tggacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag 601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg 661 gctagcgcta ccggtcgcca ccatggaccc gggtaccgtc aatctgatta aggaagatat 721 gagggttaag gtgcatatgg aagggaatgt aaacgggcat gcttttgtga ttgaagggga 781 aggaaaagga aagccctacg aagggacaca gaccgcgaac ctgacagtga aagaaggcgc 841 gcctctccca ttttcttacg acatcttgac aacagcagtg cactacggaa acagagtatt 901 caccaaatac cctgaagata taccggatta tttcaagcag tcgtttcctg aaggatattc 961 ctgggaaaga accatgactt ttgaagacaa gggcatttgt accatcagaa gcgacataag 1021 cttggaaggt gactgctttt tccaaaacgt tcgttttaag gggaccaact ttcccccaaa 1081 tggtccagtt atgcagaaga aaactttgaa gtgggaacca tccacagaga agctgcacgt 1141 gcgtgatggg ttgcttgtcg gtaatattaa catggctctg ctgcttgaag gaggtggaca 1201 ttacctgtgt gacttcaaaa ctacttacaa agcgaagaag gttgttcagt tgccagatgc 1261 ccattttgtg gaccatcgca ttgagatctt gggtaatgac agcgattaca acaaagtgaa 1321 gctgtacgag catgccgttg ctcgctattc tccgttgcca agtcaggcct ggtccggact 1381 cagatctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc cgggatccac 1441 cggatctaga atgggcaccc gcgacgacga gtacgactac ctctttaaag ttgtccttat 1501 tggagattct ggtgttggaa agagtaatct cctgtctcga tttactcgaa atgagtttaa 1561 tctggaaagc aagagcacca ttggagtaga gtttgcaaca agaagcatcc aggttgatgg 1621 aaaaacaata aaggcacaga tatgggacac agcagggcaa gagcgatatc gagctataac 1681 atcagcatat tatcgtggag ctgtaggtgc cttattggtt tatgacattg ctaaacatct 1741 cacatatgaa aatgtagagc gatggctgaa agaactgaga gatcatgctg atagtaacat 1801 tgttatcatg cttgtgggca ataagagtga tctacgtcat ctcagggcag ttcctacaga 1861 tgaagcaaga gcttttgcag aaaagaatgg tttgtcattc attgaaactt cggccctaga 1921 ctctacaaat gtagaagctg cttttcagac aattttaaca gagatttacc gcattgtttc 1981 tcagaagcaa atgtcagaca gacgcgaaaa tgacatgtct ccaagcaaca atgtggttcc 2041 tattcatgtt ccaccaacca ctgaaaacaa gccaaaggtg cagtgctgtc agaacatcta 2101 aggcattgga tccgagctcg gtaccaagct tacgtagaac aaaaactcat ctcagaagag 2161 gatctgaata gcgccgtcga ccatcatcat catcatcatt gagtttaaac ggtctccagc 2221 ttaagtttaa accgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 2281 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 2341 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 2401 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg 2461 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac 2521 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 2581 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 2641 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 2701 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 2761 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 2821 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 2881 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 2941 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 3001 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 3061 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 3121 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 3181 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 3241 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg 3301 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga 3361 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct 3421 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc 3481 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg 3541 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg 3601 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc 3661 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc 3721 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg 3781 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc 3841 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg 3901 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc 3961 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag 4021 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg 4081 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg 4141 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc 4201 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt 4261 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 4321 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 4381 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 4441 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata 4501 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 4561 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 4621 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 4681 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 4741 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 4801 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 4861 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 4921 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 4981 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 5041 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 5101 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 5161 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 5221 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 5281 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 5341 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 5401 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 5461 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 5521 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 5581 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 5641 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 5701 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 5761 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 5821 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 5881 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 5941 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 6001 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 6061 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 6121 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 6181 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 6241 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 6301 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 6361 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 6421 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 6481 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 6541 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 6601 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc 6661 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag 6721 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa 6781 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt 6841 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgtt //