LOCUS Exported 7255 bp ds-DNA circular SYN 24-MAY-2022 DEFINITION Retroviral expression of mouse Kat5. ACCESSION . VERSION . KEYWORDS pLPC-MYC.Mm.KAT5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7255) AUTHORS Barry RM, Sacco O, Mameri A, Stojaspal M, Kartsonis W, Shah P, De Ioannes P, Hofr C, Cote J, Sfeir A TITLE Rap1 regulates TIP60 function during fate transition between two-cell-like and pluripotent states. JOURNAL Genes Dev. 2022 Feb 24. pii: gad.349039.121. doi: 10.1101/gad.349039.121. PUBMED 35210222 REFERENCE 2 (bases 1 to 7255) AUTHORS . TITLE Direct Submission JOURNAL Exported May 24, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7255 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(11..28) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(182..770) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(262..281) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(930..1790) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1553..1572 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1791..1895) /gene="bla" /label=AmpR promoter LTR 1926..2395 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 2460..2659 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 2860..3276 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 3206..3228 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 3244..3260 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" CDS 3294..3893 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(3294..3313) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 3790..3810 /label=Puro-F /note="Puromycin resistance gene, forward primer" primer_bind complement(3982..4001) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 4173..4552 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 4553..4756 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 4707..4727 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 4801..4820 /label=T7 /note="T7 promoter, forward primer" promoter 4801..4819 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 4851..4860 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 4860..4889 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" LTR 6667..7136 /note="long terminal repeat from Moloney murine leukemia virus" ORIGIN 1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 841 tcatgagatt atcaaaaagg atcttcacct agatcctttt gcggccgcaa atcaatctaa 901 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 961 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 1021 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 1081 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 1141 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 1201 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 1261 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 1321 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 1381 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 1441 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 1501 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 1561 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 1621 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 1681 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 1741 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 1801 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 1861 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 1921 gcagcctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg 1981 gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc 2041 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt 2101 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt 2161 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc 2221 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc 2281 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg 2341 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg 2401 gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggca 2461 agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg 2521 cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact 2581 gacgagttct gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt 2641 ttttgtggcc cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg 2701 gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg 2761 gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt 2821 ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc 2881 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta 2941 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc 3001 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc 3061 ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc 3121 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct 3181 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc 3241 tccctttatc cagccctcac tccttctcta ggcgccggcc ggatcagctt accatgaccg 3301 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc 3361 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca 3421 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca 3481 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg 3541 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc 3601 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt 3661 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg 3721 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct 3781 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg 3841 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc 3901 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg gatctcccga 3961 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct 4021 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac 4081 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct 4141 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata 4201 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact 4261 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 4321 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 4381 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc 4441 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg 4501 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg 4561 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct 4621 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa 4681 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt 4741 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat 4801 taatacgact cactataggg agacccaagc ttggtaccga gctcggatca gccaccatgg 4861 aacaaaagtt gatttctgaa gaagatttgt taagaaagag aggatccgcg gaggtggggg 4921 agataatcga gggctgccgc ctgcccgtgc tgcggcgcaa ccaggacaac gaagatgagt 4981 ggcccctggc tgagatcctg agcgtgaagg acatcagtgg ccgaaagctt ttctatgtcc 5041 attacattga cttcaacaaa cgtctggatg aatgggtgac tcacgagcgg ctggacttaa 5101 agaagatcca atttcccaag aaagaggcca agacacctac caagaacgga cttcctgggt 5161 cccgccccgg ctctcccgaa agagaggtgc cggcctccgc ccaggccagc gggaagacct 5221 tgccaatccc ggtccagatc acactccgct tcaacctgcc caaggagcgg gaggccatcc 5281 caggtggcga gcctgaccag ccgctctcct ccagctcctg cctgcaaccc aaccaccgct 5341 caacgaaacg gaaggtggag gtggtttcac cagcaacccc agtgcccagc gagacagccc 5401 cagcctcggt tttccctcag aatgggtcag cccgtagggc agtggcagcc cagcctggac 5461 ggaagcggaa atctaattgc ttgggcactg atgaggattc tcaggacagc tcagatggaa 5521 taccgtcagc accacgaatg actggcagtc tggtgtctga ccggagccac gacgacattg 5581 tcacccggat gaagaacatt gagtgtattg agcttggccg gcaccgcctc aagccgtggt 5641 acttctcccc gtacccacaa gagcttacca cgctacccgt cctctacctg tgcgaatttt 5701 gcctcaaata tggccgtagc ctcaagtgtc tgcaacgcca cttgaccaaa tgtgatcttc 5761 ggcaccctcc aggcaatgaa atttaccgca agggcaccat ctcctttttt gagattgatg 5821 gacggaaaaa caagagttac tcacaaaacc tgtgtcttct ggccaagtgt ttcctggacc 5881 acaaaacact gtactatgac actgacccct tcctcttcta cgtaatgacg gagtatgact 5941 gcaaaggttt ccacatcgtg ggctacttct ccaaggaaaa ggaatccaca gaagattaca 6001 atgtggcctg catcttgact ctgcctccct accagcgccg gggctatggc aagctgctta 6061 ttgagttcag ctatgaactc tcgaaagtag aagggaagac cggaactcct gagaaacccc 6121 tgtcagatct tggcctccta tcctaccgaa gttactggtc ccaaaccatc ttggagatcc 6181 tgatggggct gaagtcggag agcggggaga ggccacagat caccatcaat gagatcagtg 6241 aaatcactag tatcaagaaa gaagatgtca tctccacact gcagtatctc aacctcatca 6301 attactacaa gggccagtat atcctaactc tgtcagaaga catcgtggat gggcatgagc 6361 gggctatgct caagcggctc cttcggattg actccaagtg tctgcacttc actcccaaag 6421 actggagcaa gagaggaaag tggtaagaat tctgcagata tccatcacac tggcggccgc 6481 tcgagcatgc atctagaggg ccgataaaat aaaagatttt atttagtctc cagaaaaagg 6541 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca 6601 aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg 6661 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag 6721 ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt 6781 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt 6841 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct 6901 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag 6961 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg 7021 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct 7081 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcacatg 7141 cagcatgtat caaaattaat ttggtttttt ttcttaagta tttacattaa atggccatag 7201 ttgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgct //