LOCUS Exported 9860 bp ds-DNA circular SYN 27-MAY-2022 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS FUW-TetO-Gata4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9860) AUTHORS Buganim Y, Itskovich E, Hu YC, Cheng AW, Ganz K, Sarkar S, Fu D, Welstead GG, Page DC, Jaenisch R TITLE Direct Reprogramming of Fibroblasts into Embryonic Sertoli-like Cells by Defined Factors. JOURNAL Cell Stem Cell. 2012 Sep 7;11(3):373-86. PUBMED 22958931 REFERENCE 2 (bases 1 to 9860) AUTHORS . TITLE Direct Submission JOURNAL Exported May 27, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9860 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 133..512 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 514..712 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 667..687 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 730..910 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 957..1082 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1575..1808 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1993..2037 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2335..2452 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 2509..2527 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2551..2569 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2593..2611 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2635..2653 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2677..2695 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2719..2737 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" promoter 2770..2808 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2805..2829 /label=LNCX /note="Human CMV promoter, forward primer" misc_feature 4447..5035 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4500..4520) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4918..4929) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(5038..5054) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5039..5055) /label=pBluescriptKS /note="For pBluescript vector" LTR 5560..5740 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(5766..5783) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5772..5996 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6042..6470 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6129..6148) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6339..6360 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(6479..6499) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 6484..6813 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 6664..6799 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6726..6745 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 6861..6908 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 6927..7301 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 7431..7552 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7468..7487) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7522..7541 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(7601..7617) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7601..7617) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7614..7636) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7625..7641 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7649..7679) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7694..7715 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7832..7849) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8003..8591) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8083..8102) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8762..9622) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9385..9404 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9623..9727) /gene="bla" /label=AmpR promoter primer_bind complement(9802..9821) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca 61 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca 121 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat 181 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg 241 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa 301 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact 361 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta 421 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt 481 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg 541 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg 601 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc 661 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt 721 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta 781 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc 841 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa 901 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct 961 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg 1021 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg 1081 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg 1141 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg 1201 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac 1261 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc 1321 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag 1381 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct 1441 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa 1501 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa 1561 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg 1621 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag 1681 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc 1741 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa 1801 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg 1861 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag 1921 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa 1981 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg 2041 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga 2101 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag 2161 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc 2221 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac 2281 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa 2341 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 2401 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta 2461 ttacagggac agcagagatc cagtttggtt agatctcgag tttaccactc cctatcagtg 2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt 2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta 2641 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt 2701 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agctcggtac 2761 ccgggtcgag gtaggcgtgt acggtgggag gcctatataa gcagagctcg tttagtgaac 2821 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac 2881 cgatccagcc tccgcggccc cgaattcgcc ctttttgggt ttttgttttg ttttgttttg 2941 ttttgatttt tggtgacagt tccgcacacc cgcattctag ttcttgtctg cctcgtgctc 3001 agagcttggg gcgatgtacc aaagcctggc catggccgcc aaccacggcc ccccgcccgg 3061 cgcctacgaa gcaggtggcc ctggcgcctt catgcacagc gcgggcgccg cgtcctcgcc 3121 cgtctacgtg cccactccgc gggtgccgtc ctctgtgctg ggcctgtcct acctgcaggg 3181 cggtggcagt gccgctgcag ctggaaccac ctcgggtggc agctccgggg ccggcccgtc 3241 gggtgcaggg cctgggaccc agcagggtag ccctggctgg agccaagctg gagccgaggg 3301 agccgcctac accccgccgc ccgtgtcccc gcgcttctct ttcccgggga ctactgggtc 3361 cctggcggcc gctgccgccg ctgccgcagc ccgggaagct gcagcctacg gcagtggcgg 3421 cggggcggcg ggcgctggtc tggctggccg agagcagtac gggcgtccgg gcttcgccgg 3481 ctcctactcc agcccctacc cagcctacat ggccgacgtg ggagcatcct gggccgcagc 3541 cgctgccgcc tctgccggcc ccttcgacag cccagtcctg cacagcctgc ctggacgggc 3601 caaccctgga agacacccca atctcgtaga tatgtttgat gacttctcag aaggcagaga 3661 gtgtgtcaat tgtggggcca tgtccacccc actctggagg cgagatggga cgggacacta 3721 cctgtgcaat gcctgtggcc tctatcacaa gatgaacggc atcaaccggc ccctcattaa 3781 gcctcagcgc cgcctgtccg cttcccgccg ggtaggcctc tcctgtgcca actgccagac 3841 taccaccacc acgctgtggc gtcgtaatgc cgagggtgag cctgtatgta atgcctgcgg 3901 cctctacatg aagctccatg gggttcccag gcctcttgca atgcggaagg aggggattca 3961 aaccagaaaa cggaagccca agaacctgaa taaatctaag acgccagcag gtcctgctgg 4021 tgagaccctc cctccctcca gtggtgcctc cagcggtaac tccagcaatg ccactagcag 4081 cagcagcagc agtgaagaga tgcgccccat caagacagag cccgggctgt catctcacta 4141 tgggcacagc agctccatgt cccagacatt cagtactgtg tccggccacg ggccctccat 4201 ccatccagtg ctgtctgctc tgaagctgtc cccacaaggc tatgcatctc ctgtcactca 4261 gacatcgcag gccagctcca agcaggactc ttggaacagc ctggtcctgg ctgacagtca 4321 tggggacata atcaccgcgt aatcagcgcc cccccttccc tcttcaaatt cctgctcgga 4381 cttgggacgt gggggccagc aaagtaaaag gctgggaagg gcgaattcga tatcaagctt 4441 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 4501 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 4561 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 4621 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 4681 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 4741 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 4801 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg 4861 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 4921 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 4981 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga 5041 taccgtcgac ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct 5101 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc 5161 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 5221 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt 5281 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg 5341 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa 5401 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg 5461 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca 5521 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag 5581 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 5641 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 5701 tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg 5761 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc 5821 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc 5881 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa 5941 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc 6001 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc 6061 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 6121 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 6181 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 6241 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 6301 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 6361 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 6421 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg 6481 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 6541 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 6601 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 6661 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 6721 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga 6781 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt 6841 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat 6901 aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca 6961 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg 7021 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg 7081 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg 7141 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc 7201 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg 7261 ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg 7321 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 7381 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta 7441 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 7501 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 7561 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 7621 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 7681 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 7741 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 7801 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 7861 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 7921 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 7981 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 8041 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 8101 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 8161 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 8221 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 8281 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 8341 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 8401 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 8461 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 8521 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 8581 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 8641 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 8701 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 8761 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 8821 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 8881 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 8941 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 9001 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 9061 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 9121 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 9181 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 9241 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 9301 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 9361 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 9421 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 9481 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 9541 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 9601 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 9661 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 9721 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga 9781 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct 9841 gctccctgct tgtgtgttgg //