LOCUS Exported 9886 bp ds-DNA circular SYN 27-MAY-2022 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS FUW-TetO-Nr5a1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9886) AUTHORS Buganim Y, Itskovich E, Hu YC, Cheng AW, Ganz K, Sarkar S, Fu D, Welstead GG, Page DC, Jaenisch R TITLE Direct Reprogramming of Fibroblasts into Embryonic Sertoli-like Cells by Defined Factors. JOURNAL Cell Stem Cell. 2012 Sep 7;11(3):373-86. PUBMED 22958931 REFERENCE 2 (bases 1 to 9886) AUTHORS . TITLE Direct Submission JOURNAL Exported May 27, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9886 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 172..551 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 553..751 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 706..726 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 769..949 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 996..1121 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1614..1847 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2032..2076 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2374..2491 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 2548..2566 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2590..2608 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2632..2650 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2674..2692 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2716..2734 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2758..2776 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" promoter 2809..2847 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2844..2868 /label=LNCX /note="Human CMV promoter, forward primer" misc_feature 4512..5100 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4565..4585) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4983..4994) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(5103..5119) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5104..5120) /label=pBluescriptKS /note="For pBluescript vector" LTR 5625..5805 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(5831..5848) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5837..6061 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6107..6535 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6194..6213) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6404..6425 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(6544..6564) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 6549..6878 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 6729..6864 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6791..6810 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 6926..6973 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 6992..7366 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 7496..7617 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7533..7552) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7587..7606 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(7666..7682) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7666..7682) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7679..7701) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7690..7706 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7714..7744) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7759..7780 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7897..7914) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8068..8656) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8148..8167) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8827..9687) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9450..9469 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9688..9792) /gene="bla" /label=AmpR promoter primer_bind complement(9867..9886) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga 61 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta 121 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat 181 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg 241 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc 301 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt 361 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc 421 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 481 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 541 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 601 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 661 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 721 ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg 781 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 841 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 901 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 961 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 1021 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 1081 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 1141 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 1201 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 1261 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 1321 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 1381 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 1441 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac 1501 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca 1561 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct 1621 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg 1681 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg 1741 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag 1801 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt 1861 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa 1921 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat 1981 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa 2041 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat 2101 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 2161 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 2221 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt 2281 ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca 2341 attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg 2401 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt 2461 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc 2521 agtttggtta gatctcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga 2581 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca 2641 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa 2701 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc 2761 ctatcagtga tagagaaaag tgaaagtcga gctcggtacc cgggtcgagg taggcgtgta 2821 cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc 2881 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggcccc 2941 gaattcgccc tttccagtgt ccacccttat ccggctgaga attctccttc cgttcagcgg 3001 acgccgcggg catggactat tcgtacgacg aggacctgga cgagctgtgt ccagtgtgtg 3061 gtgacaaggt gtcgggctac cactacgggc tgctcacgtg cgagagctgc aagggcttct 3121 tcaagcgcac agtccagaac aacaagcatt acacgtgcac cgagagtcag agctgcaaaa 3181 tcgacaagac gcagcgtaag cgctgtccct tctgccgctt ccagaagtgc ctgacggtgg 3241 gcatgcgcct ggaagctgtg cgtgctgatc gaatgcgggg tggccggaac aagtttgggc 3301 ccatgtacaa gagagaccgg gccttgaagc agcagaagaa agcacagatt cgggccaatg 3361 gcttcaagct ggagaccgga ccaccgatgg gggtgccccc gccaccccct cccccaccgg 3421 actacatgtt accccctagc ctgcacgcac cggagcccaa ggccctggtc tctggcccac 3481 ccagtgggcc gctgggtgac tttggagccc catctctacc catggctgtg cctggtcccc 3541 acggacctct ggctggctac ctctatcctg ccttctctaa ccgcaccatc aagtctgagt 3601 atccagagcc ctatgccagc cccccacaac agccagggcc accctacagc tatccagagc 3661 ccttctcagg agggcccaat gtaccagagc tcatattgca gctgctgcaa ctagagccag 3721 aggaggacca ggtgcgcgct cgcatcgtgg gctgtctgca ggagccagcc aaaagccgct 3781 ctgaccagcc agcgcccttc agcctcctct gcagaatggc cgaccagacc tttatctcca 3841 ttgtcgactg ggcacgaagg tgcatggtct ttaaggagct ggaggtggct gaccagatga 3901 cactgctgca gaactgttgg agcgagctgc tggtgttgga ccacatctac cgccaggtcc 3961 agtacggcaa ggaagacagc atcctgctgg ttactggaca ggaggtggag ctgagcacag 4021 tggctgtgca ggctggctcc ctgctgcaca gcctggtgct gcgggcccaa gagttagtgc 4081 tccagttgca tgcactgcag ctggaccgcc aggagttcgt ctgtctcaag ttcctcatcc 4141 tcttcagcct cgatgtgaaa ttcctgaaca accacagcct cgtaaaggac gcccaggaaa 4201 aggccaacgc tgccctgttg gattacacct tgtgtcacta cccacactgc ggggacaaat 4261 tccagcagtt gctattgtgc ctggtggagg tgcgggccct gagcatgcag gccaaggagt 4321 acctgtacca caagcatttg ggcaacgaga tgccccgcaa caaccttctc attgagatgc 4381 tgcaggccaa gcagacttga gcctgggtgc caggcagcgg gcaataggca gggatgccac 4441 tgcctccaaa agactccttg cattaggtga tccaggagcc caagggcgaa ttcgatatca 4501 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 4561 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 4621 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 4681 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 4741 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 4801 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 4861 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc 4921 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 4981 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 5041 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 5101 atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag 5161 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc 5221 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 5281 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata 5341 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac 5401 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg 5461 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga 5521 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc 5581 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag 5641 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 5701 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 5761 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc 5821 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc 5881 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa 5941 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac 6001 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg 6061 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc 6121 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 6181 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 6241 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 6301 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 6361 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 6421 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 6481 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc 6541 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta 6601 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag 6661 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 6721 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 6781 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt 6841 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat 6901 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat 6961 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt 7021 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc 7081 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat 7141 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg 7201 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc 7261 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga 7321 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga 7381 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 7441 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt 7501 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 7561 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 7621 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 7681 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 7741 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 7801 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 7861 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 7921 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 7981 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 8041 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 8101 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 8161 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 8221 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 8281 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 8341 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 8401 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 8461 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 8521 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 8581 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 8641 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 8701 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 8761 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 8821 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 8881 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 8941 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 9001 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 9061 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 9121 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 9181 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 9241 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 9301 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 9361 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 9421 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 9481 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 9541 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 9601 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 9661 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 9721 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 9781 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct 9841 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccg //