LOCUS Exported 6605 bp ds-DNA circular SYN 16-JUN-2022 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Flag-SENP1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6605) AUTHORS Cheng J, Kang X, Zhang S, Yeh ET TITLE SUMO-specific protease 1 is essential for stabilization of HIF1alpha during hypoxia. JOURNAL Cell. 2007 Nov 2. 131(3):584-95. PUBMED 17981124 REFERENCE 2 (bases 1 to 6605) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 16, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6605 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(38..57) /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind 284..304 /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" polyA_signal 328..950 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind 732..751 /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" CDS complement(2938..2961) /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" promoter complement(2995..3198) /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind complement(3024..3044) /label=CMV-F /note="Human CMV immediate early promoter, forward primer" enhancer 3199..3578 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" primer_bind complement(3739..3756) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(3739..3755) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3748..3770) /label=M13/pUC Forward /note="In lacZ gene" rep_origin complement(3896..4351) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4033..4054) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 4245..4264 /label=F1ori-R /note="F1 origin, reverse primer" promoter 4378..4482 /gene="bla" /label=AmpR promoter CDS 4483..5343 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4701..4720) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5514..6102 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6003..6022 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6256..6273 /label=L4440 /note="L4440 vector, forward primer" protein_bind 6390..6411 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 6426..6456 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6464..6480 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6469..6491 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 6488..6504 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 6488..6504 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 6518..6537 /label=T7 /note="T7 promoter, forward primer" promoter 6518..6536 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter complement(6575..299) /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 6589..119 /label=SV40 ori /note="SV40 origin of replication" ORIGIN 1 cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa 61 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg 121 ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt 181 ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg 241 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca 301 ttccacagcc aagctggccg cgtacccaat tcaacaggca tctactgagt ggacccaacg 361 catgagagga cagtgccaag caagcaactc aaatgtccca ccggttgggc atggccaggt 421 agcctatgct gtgtctggac gtcctcctgc tggtatagtt attttaaaat cagaaggaca 481 gggaagggag cagtggttca cgcctgtaat cccagcaatt tgggaggcca aggtgggtag 541 atcacctgag attaggagtt ggagaccagc ctggccaata tggtgaaacc ccgtctctac 601 caaaaaaaca aaaattagct gagcctggtc atgcatgcct ggaatcccaa caactcggga 661 ggctgaggca ggagaatcgc ttgaacccag gaggcggaga ttgcagtgag ccaagattgt 721 gccactgcac tccagcttgg ttcccaatag accccgcagg ccctacaggt tgtcttccca 781 acttgcccct tgctccatac cacccccctc caccccataa tattatagaa ggacacctag 841 tcagacaaaa tgatgcaact taattttatt aggacaaggc tggtgggcac tggagtggca 901 acttccaggg ccaggagagg cactggggag gggtcacagg gatgccaccc gggatcctct 961 agagtcgact catcacaaga gttttcggtg gaggatctcc cagaccatcc gcttccggaa 1021 gtatggcatg tgttgctgtg cgaagttgat tggtctgtct ttggtaatac agtcagcata 1081 tttgcaggca aacatcccac agtcacttcc attcatctgc tgaggaatct cctggctttt 1141 cttgctgaaa agctgccagc cattggtgtc aaactctttc cttttcttgt caatgctttc 1201 ttgctttagg tattgcaaga gtattctgca ggcttcattg tttatcccac ccatggagtc 1261 gtaataggta atattcttct ttctaaagtc cacaacagct agacaccagt gtactcccag 1321 gtgaatgggc accaaaagaa tgtcaacaga aaatacatct actttctttg tccaacgttt 1381 cactgcctga taaccagccg tttttaattt agtgaagaaa aaggtattaa atgcatgcac 1441 acttggcaag cccttctctt tacttcgctc catcagcata ttcatgtaga aattgatgat 1501 ctcatcattg agccaattca gatggtttag agtttgaata tctttgcgtg taatggtcag 1561 gcgaaatgct tcactgagaa cttcatcctg attcccatta cgaaatacat tctttatttc 1621 tttctccatt tcctctgtaa tttcaggaaa ttcatcttca ctatcagtta atttatgacc 1681 ttttttttgt gtttcttgga caacagtaac aggaatctcc ttttcaagag gtacacgaag 1741 atgtagttct actgaatcat gtactgaatg ttcccgctcc tgcaatctct ggttttgaag 1801 ctgtaaggcc aatgccttct gttcttcaat ctggcgcaat ctttctcgtg ctcgagaatc 1861 ataaacacta gttaattctt tgatccacag ctctgcctgg aagaaagtag aactgggagt 1921 tggagtctgg gaatctttca ctttcagtaa aatcacagag tctgatcctt cagattgtgt 1981 atttgaagct gctaagttat ctggctgatg tggaacagag tggtgatgat ggggatgatg 2041 aagagtacca gaatctttgg atccaaatgc aacatctggg gtataagaag atagggaata 2101 tacactgtgg gacagctgtt cctggttagt taaaatgctg gcagatccag atgatgaagt 2161 atcagagcca atgatctgag atgcacaaga gcttccattt ttaaacagtg agtctttcaa 2221 agtattttta ctggaactaa gacatcgaga caggtgtaaa ggaaaatgtg tggtgggttt 2281 ggctatagca aactgtttcc ctgtgaccat ctgtagcagc tgtctgtaaa actctctttc 2341 ttcttcttga actgtctctt ctgctgtact aacatgtcgc ctctgagttt tcttggggct 2401 caaaagactt cgacgacatg aaccactcca agatggactt ggaacaggtt taataggaaa 2461 agatttttca tatgcagata catggcagtg atggtttgac tttcccgcaa aactgtttga 2521 taatccactt gaggtctttc gggtttcgag gtaaagactt cggctgtttc ttgatttttg 2581 taaagatgag cttgacgatg gggtagaatt tctccattgg ccatttgcac tctggccaaa 2641 ggttcttaaa tcgcctgagc caagaaaact gtctgaggaa ggattatctg agtaatagct 2701 tggattataa gctgcacttc ttgtggaaca tgtaaaagat cggtccaaat gtccttgcct 2761 ggaagataaa atctgctggt cagaaagcga aagctggtcc tctggaaaac ctgtttgtgg 2821 caggaggtgg gttttgaata cggagttgtg gttcactaaa gtcacttctc cagcatccat 2881 cctcatccta tcagcaatat catccataag atctatcgat gaattcgcgg ccgcaagctt 2941 gtcgtcatcg tctttgtagt ccatggtaga tcaattctga cggttcacta aacgagctct 3001 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaacg gggcggggtt 3061 attacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc 3121 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattggtgta 3181 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt 3241 aggaaagtcc cgtaaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt 3301 gacgtcaata gggggcggac ttggcatatg atacacttga tgtactgcca agtgggcagt 3361 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg 3421 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc 3481 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt 3541 aattgattac tattaataac tagtcaataa tcaatgtcaa catggcggtc atattggaca 3601 tgagccaata taaatgtaca tattatgata tagatacaac gtatgcaatg gccaatagcc 3661 aatattgatt tatgctatat aaccaatgac taatatggct aattgccaat attgattcaa 3721 tgtatagatc agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 3781 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 3841 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt 3901 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt 3961 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg 4021 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc 4081 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca 4141 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga 4201 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa 4261 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc 4321 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc 4381 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 4441 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 4501 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 4561 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 4621 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 4681 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 4741 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 4801 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 4861 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 4921 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 4981 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 5041 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 5101 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 5161 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 5221 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 5281 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 5341 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 5401 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 5461 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 5521 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 5581 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 5641 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 5701 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 5761 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 5821 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 5881 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 5941 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 6001 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 6061 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 6121 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 6181 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 6241 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa 6301 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 6361 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc 6421 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa 6481 tttcacacag gaaacagcta tgacatgatt acgaatttaa tacgactcac tatagggaat 6541 taactttctg gtttttcagt tcctcgagga gctttttgca aaagcctagg cctccaaaaa 6601 agcct //