LOCUS       Exported               10834 bp ds-DNA     circular SYN 17-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    HA Raptor
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10834)
  AUTHORS   Kim DH, Sarbassov DD, Ali SM, King JE, Latek RR, Erdjument-Bromage 
            H, Tempst P, Sabatini DM
  TITLE     mTOR interacts with raptor to form a nutrient-sensitive complex that
            signals to the cell growth machinery.
  JOURNAL   Cell 2002 Jul 26;110(2):163-75.
  PUBMED    12150925
REFERENCE   2  (bases 1 to 10834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 17, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10834
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      94..103
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     polyA_signal    107..241
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(144..163)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     198..217
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        310..667
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(333..353)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      518..653
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     580..599
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(687..704)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(687..703)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(696..718)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      916..1371
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1003..1022)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1213..1234
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1388..1407)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1507..1529
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1567..1585)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1653..1757
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1758..2618
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1976..1995)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2789..3377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3278..3297
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3531..3548
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3665..3686
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3701..3731
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3739..3755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3744..3766
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3763..3779
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3763..3779
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        3808..4187
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4188..4391
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4342..4362
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4388..4412
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        4622..4640
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4622..4639
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     4653..4672
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      4757..4766
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4769..4795
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aagagcagag agtgaccaac agtaaacaac acgcgcagac tcaaaaaaaa aaaaaaaagg
       61 gcggccgcta agtaagtaag gatccccagc ttggccgcca tggcccaact tgtttattgc
      121 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
      181 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
      241 cgggaattaa ttcggcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt
      301 aggtaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
      361 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
      421 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      481 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
      541 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
      601 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
      661 ttgcaaaaag ctgttaacag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa
      721 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      781 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      841 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
      901 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      961 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     1021 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     1081 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     1141 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     1201 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct
     1261 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     1321 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     1381 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     1441 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     1501 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     1561 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     1621 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1681 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1741 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     1801 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     1861 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     1921 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     1981 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     2041 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     2101 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     2161 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     2221 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     2281 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     2341 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     2401 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     2461 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2521 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2581 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2641 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     2701 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2761 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2821 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2881 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2941 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3001 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3061 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3121 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3181 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3241 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3301 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3361 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3421 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3481 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3541 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3601 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     3661 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     3721 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca
     3781 tgattacgaa ttaattcgag ctcgcccgac attgattatt gactagttat taatagtaat
     3841 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     3901 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     3961 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     4021 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     4081 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     4141 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     4201 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     4261 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     4321 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     4381 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     4441 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     4501 cgcggattcc ccgtgccaag agtgacgtaa gtaccgccta tagagtctat aggcccaccc
     4561 ccttggcttc gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac
     4621 gatttaggtg acactataga ataacatcca ctttgccttt ctctccacag gtgtccactc
     4681 ccaggtccaa ctgcacctcg gttctatcga ttgaattccc gaaccgacag tcggtctctt
     4741 caccaaggcc attcgcgcca ccatgggcta cccctatgat gtgcctgact acgcagatct
     4801 caatggtggt ggtggtgggt cgacgatgga gtccgaaatg ctgcaatcgc ctcttctggg
     4861 cctgggggag gaagatgagg ctgatcttac agactggaac ctacctttgg cttttatgaa
     4921 aaagaggcac tgtgagaaaa ttgaaggctc caaatcctta gctcagagct ggaggatgaa
     4981 ggatcggatg aagacagtca gtgttgcctt agttttgtgc ctgaatgttg gtgtggaccc
     5041 tcccgatgtg gtgaagacca cgccctgtgc acgcttggaa tgctggatcg atcctctgtc
     5101 gatgggtcct cagaaagctc tggaaaccat cggtgcaaat ttacagaagc agtacgagaa
     5161 ctggcagcca agggcccggt acaagcagag ccttgaccca actgtggatg aagtcaagaa
     5221 gctctgcacg tccttacgtc gcaacgccaa ggaggagcga gtcctctttc actacaatgg
     5281 ccacggggtg ccccggccca cagtcaacgg ggaggtctgg gtcttcaaca agaactacac
     5341 gcagtacatc cctctgtcca tatatgacct gcagacgtgg atgggcagcc cgtcgatctt
     5401 cgtctacgac tgctccaatg ctggcttgat cgtcaagtcc ttcaagcagt tcgcactaca
     5461 gcgggagcag gagctggagg tagctgcaat caacccaaat caccctcttg ctcagatgcc
     5521 tttgcctccg tcgatgaaaa actgcatcca gctggcagcc tgcgaggcca ccgagctgct
     5581 gcccatgatc cccgacctcc cggctgacct attcacctcc tgcctcacca cccccatcaa
     5641 gatcgccctg cgctggtttt gcatgcagaa atgtgtcagt ctggtgcctg gcgtcacact
     5701 ggatttgata gaaaagatcc ctggccgcct gaacgacagg aggacgcccc tgggtgaact
     5761 gaactggatc ttcacagcca tcacagacac catcgcgtgg aacgtgctcc cccgggatct
     5821 cttccaaaag ctcttcagac aggacttgct ggtggctagt ctgtttcgaa attttttatt
     5881 ggcggaaagg attatgaggt cgtataactg cactcccgtc agcagcccgc gtctgccgcc
     5941 cacgtacatg cacgccatgt ggcaagcctg ggacctggct gttgacatct gtctgtctca
     6001 gctgccgacg atcatcgagg aaggcactgc gtttcggcac agcccgttct tcgccgagca
     6061 gctgaccgca ttccaggtgt ggctcaccat gggcgtggag aaccgaaacc cacccgaaca
     6121 gctgcccatc gtcctgcagg tgctgttaag ccaagtgcac cggctgagag cattggactt
     6181 gcttggaaga tttttggacc tgggtccctg ggcagtgagc ctggccttgt ctgtcggcat
     6241 cttcccctac gtgctgaagc tgctccagag ctcggcccga gagctgcggc cacttctcgt
     6301 tttcatctgg gccaagatcc tcgcagtgga cagctcgtgc caggcggacc tcgtgaagga
     6361 caacggccac aagtacttcc tgtcggtcct ggcggacccc tacatgccag ctgaacaccg
     6421 gaccatgacg gctttcattc tcgccgtgat cgtcaacagc tatcacacgg ggcaggaagc
     6481 ctgccttcag ggaaacctca ttgccatctg cctggagcag ctcaacgacc cgcacccctt
     6541 gctgcgccag tgggtggcca tctgcctcgg caggatctgg cagaacttcg actcggcgag
     6601 gtggtgcggc gtgagggaca gcgctcatga gaagctctac agcctcctct ccgaccccat
     6661 tcccgaggtc cgctgcgcag cggtcttcgc ccttggcacg ttcgtgggca actctgcaga
     6721 gaggacggac cactccacca ccatcgacca caacgtggcc atgatgctgg cccagctggt
     6781 cagcgacggg agccccatgg tccggaagga gctggtggtg gctctgagtc atcttgtggt
     6841 tcagtatgaa agcaatttct gcaccgtggc cctgcagttc atagaagagg aaaagaacta
     6901 cgccttgcct tctccagcaa ccacagaggg agggagtttg accccagtgc gagacagccc
     6961 gtgcaccccc agacttcgtt ctgtgagctc ctatggaaac atccgtgctg tcgccacagc
     7021 caggagcctc aacaaatctt tgcagaacct gagtttgaca gaggaatctg gtggcgcggt
     7081 ggcgttctcc cccggaaacc tcagcaccag cagcagcgcc agcagcaccc tgggcagccc
     7141 cgagaatgag gagcatatcc tgtccttcga gaccatcgac aagatgcgcc gcgccagctc
     7201 ctactcctcc ctcaactccc tcatcggagt ttcctttaac agtgtttaca ctcagatttg
     7261 gagagtcctg ctgcacctgg ctgctgaccc ctatccagag gtctcggacg tggccatgaa
     7321 agtactcaac agcatcgcct acaaggccac cgtgaacgcc cggccgcagc gcgtcctgga
     7381 cacctcctcc ctcacgcagt cggcccccgc cagccccacc aacaagggcg tgcacatcca
     7441 ccaggcgggg ggctcccctc cggcgtccag caccagcagc tccagcctga ccaacgatgt
     7501 ggccaagcag ccggtcagcc gagacttgcc ttctggccgg ccgggcacca caggccccgc
     7561 tggggcgcag tacacccctc actcccacca gttcccccgg acacggaaga tgttcgacaa
     7621 gggcccagag cagactgcgg acgacgcgga cgatgctgct ggacacaaaa gtttcatctc
     7681 cgccacggtg cagacggggt tctgcgactg gagcgcccgc tattttgccc agcccgtcat
     7741 gaagatccca gaagagcacg acctggagag tcagatccgc aaggagcggg agtggcggtt
     7801 cctgcgaaac agccgtgtca ggaggcaggc ccagcaagtc attcagaagg gcattacgag
     7861 attggacgac caaatatttc tgaacaggaa ccccggcgtc ccctctgtgg tgaaattcca
     7921 ccccttcacg ccgtgcatcg ctgtagccga caaggacagc atctgctttt gggactggga
     7981 gaaaggggag aagctggatt atttccacaa tgggaaccct cggtacacga gggtcactgc
     8041 catggagtat ctgaatggcc aggactgctc gcttctgctg acggccacag acgatggtgc
     8101 catcagggtc tggaagaatt ttgctgattt ggaaaagaac ccagagatgg tgaccgcgtg
     8161 gcaggggctc tcggacatgc tgccaacgac gcgaggagct gggatggtgg tggactggga
     8221 gcaggagacc ggcctcctca tgagctcagg agacgtgcgg atcgtccgga tctgggacac
     8281 agaccgtgag atgaaggtgc aggacatccc tacgggcgca gacagctgtg tgacgagtct
     8341 gtcctgtgat tcccaccgct cactcatcgt ggctggcctc ggtgacggct ccatccgcgt
     8401 ctacgacaga aggatggcac tcagcgaatg ccgcgtcatg acgtaccggg agcacacagc
     8461 ctgggtggtg aaggcctccc tgcagaagcg tcccgacggc cacatcgtga gtgtgagcgt
     8521 caatggagat gtgcgcatct ttgatccccg gatgcctgag tcggtaaatg tgcttcagat
     8581 cgtgaagggg ctgacggccc tggacatcca cccccaggcg gacctgatcg catgtggctc
     8641 cgtcaatcag ttcaccgcca tctacaacag cagcggagag ctcatcaaca acatcaagta
     8701 ctacgacggc ttcatgggcc agcgggtcgg cgccatcagc tgcctggcct tccacccgca
     8761 ctggcctcac ctggccgtgg gaagcaacga ctactacatc tccgtgtact cggtggagaa
     8821 gcgtgtcaga tagcggcgtg acccgggccc accaggccac ggccgcctgc tgtacatagt
     8881 gaagctgtca ctcgccgggg cacggggcgt cggctgctgc ggccccgcag tgtgaacgtt
     8941 ggctgctgcc ttagctgctg atgacggcag gagggccctg ctactcgctt ttgtctgtct
     9001 tcgctgtcgt gtctggaatg tcagggaagg ggagggctcg ggttgacggt ggcttcccac
     9061 tgagcaccag catccaggtg cacccccgcg gccacggcgc ctctgtccct ctcctgttct
     9121 gtgtttctct gagacgctga aaggggaaac acctcacttt atttccatgt aatcagagca
     9181 ttagctgcag aaaaaccccc agacagagcc ctggcggaga ggcaggcgct ggggctccta
     9241 cgggtccctg gggcagctgt ccccatcagg ccaagagcga gcgagaggcg ctgccccagc
     9301 caggcccacc acctctcaca gtcagtgcac gcaagcaggg acatttccta gccagctggg
     9361 ggacactgga aattcgggaa accaagagag aggaagaagg agacgcccct ccaactggcg
     9421 ggtgtgaagg aagccgccca ggggtccggg ctgtccttgg ccgctggcag catcactgag
     9481 caggaagcgc acagcccacc ctccccgcac ctccaggtct ctggactcca gttttggccc
     9541 ctctcacaca gagctgtcag caggggccgc tgtggcggtg cacaggggag gcaggtcctt
     9601 ggcgaggtag cccctgcctt aatccacggg gctcctttcc ctccgaaggg ctgctcttcc
     9661 ccacaggcgc ggggacagca gcccgacctg tggtctccat gcctgtgccc tcacacaggt
     9721 gtagcacacg catgtgcaga tggcaccacg gccggcacct gggggcacac acatgcaggc
     9781 ggcgtggtct ccctgctctg tccccacacg ttcctcacat acaggcaaga ggcactgccg
     9841 ggtcccggac ggctccgggt gacaccagcc ccgtctccag ccttgagccg cccatgctga
     9901 tgcgacctcg gctgacagct gggcctgtgg tgcagacagg agctgtgtgg acagtcccgc
     9961 ccaggagggg ccgcagggcg tgtatgagca gttttgcaaa cagaacacaa ccacaatgat
    10021 ggtattttga aaagtgttct ttccgtgttc gtcgggaatc aggattattg agaggtgaag
    10081 gagccaggtg gcttcattct ggcggtgaga ggcccatgac cacgggagtg agagctggtg
    10141 tggcgaggcc cggctctcct gcggtgtggc tggtggcctg ccgtggccaa gagcatcttc
    10201 tgggtggatg gaaccctgcc tggtcacatt tggccagaga cacacctggc cctcaggggg
    10261 ctgagctgga gactgagctg gggctggccg ggacgtgaca aggcaggaca gaggcggccc
    10321 ctccgctgct cctttttgga atgcgagctc ccaccagaag aaggttccgg cacgaatccc
    10381 atccccacgt ctgggccgag aaagcagccc gggtccggaa ggtgtagaga gtcccggcct
    10441 cactcagctc acagggcgtg ccaggcggca acaccagaat cttccagaag cccagctcca
    10501 cccgcacacg cagcttccca tccagtcctt caactcaatt cttacccaac acgcgtttcc
    10561 gtttgttttg agacaaaatc accacctgtc aaaaggcagg tggctccaga ggggtcaaga
    10621 cccccccccc cgcccccgct ccaccctgga gcccaccccc atgggcactg cgtgccgcct
    10681 gcacgtgggc tgtcttcaca ggtctgatgt gaaaattcaa tcacgacgtt aaccggctcg
    10741 agagagcgcc ggcctagagg ctcattatct atttatttta ccaaacgcga attgagacgg
    10801 actttgacaa aacacgaaat ggtaatgtga agct
//
