LOCUS       Exported                6856 bp ds-DNA     circular SYN 17-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Human V5-IRF3-pcDNA3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6856)
  AUTHORS   Zhu J, Smith K, Hsieh PN, Mburu YK, Chattopadhyay S, Sen GC, Sarkar 
            SN
  TITLE     High-throughput screening for TLR3-IFN regulatory factor 3 signaling
            pathway modulators identifies several antipsychotic drugs as TLR 
            inhibitors.
  JOURNAL   J Immunol. 2010 May 15;184(10):5768-76. Epub 2010 Apr 9.
  PUBMED    20382888
REFERENCE   2  (bases 1 to 6856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 17, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6856
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             24..65
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        complement(1482..1500)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1483..1500)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1520..1537)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1526..1750
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1796..2224
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1883..1902)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2093..2114
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2233..2253)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2238..2567
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2418..2553
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2480..2499
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2634..3428
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2688..2707)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3298..3317
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3602..3723
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3639..3658)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3693..3712
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3772..3788)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3772..3788)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3785..3807)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3796..3812
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3820..3850)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3865..3886
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4003..4020)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4174..4762)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4254..4273)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4933..5793)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5556..5575
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5794..5898)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5973..5992)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6164..6543
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6544..6747
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6698..6718
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6792..6811
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6792..6810
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cagtgtgctg gaattccacc atgggtaagc ctatccctaa ccctctcctc ggtctcgatt
       61 ctacgggaac cccaaagcca cggatcctgc cctggctggt gtcgcagctg gacctggggc
      121 aactggaggg cgtggcctgg gtgaacaaga gccgcacgcg cttccgcatc ccttggaagc
      181 acggcctacg gcaggatgca cagcaggagg atttcggaat cttccaggcc tgggccgagg
      241 ccactggtgc atatgttccc gggagggata agccagacct gccaacctgg aagaggaatt
      301 tccgctctgc cctcaaccgc aaagaagggt tgcgtttagc agaggaccgg agcaaggacc
      361 ctcacgaccc acataaaatc tacgagtttg tgaactcagg agttggggac ttttcccagc
      421 cagacacctc tccggacacc aatggtggag gcagtacttc tgatacccag gaagacattc
      481 tggatgagtt actgggtaac atggtgttgg ccccactccc agatccggga cccccaagcc
      541 tggctgtagc ccctgagccc tgccctcagc ccctgcggag ccccagcttg gacaatccca
      601 ctcccttccc aaacctgggg ccctctgaga acccactgaa gcggctgttg gtgccggggg
      661 aagagtggga gttcgaggtg acagccttct accggggccg ccaagtcttc cagcagacca
      721 tctcctgccc ggagggcctg cggctggtgg ggtccgaagt gggagacagg acgctgcctg
      781 gatggccagt cacactgcca gaccctggca tgtccctgac agacagggga gtgatgagct
      841 acgtgaggca tgtgctgagc tgcctgggtg ggggactggc tctctggcgg gccgggcagt
      901 ggctctgggc ccagcggctg gggcactgcc acacatactg ggcagtgagc gaggagctgc
      961 tccccaacag cgggcatggg cctgatggcg aggtccccaa ggacaaggaa ggaggcgtgt
     1021 ttgacctggg gcccttcatt gtagatctga ttaccttcac ggaaggaagc ggacgctcac
     1081 cacgctatgc cctctggttc tgtgtggggg agtcatggcc ccaggaccag ccgtggacca
     1141 agaggctcgt gatggtcaag gttgtgccca cgtgcctcag ggccttggta gaaatggccc
     1201 gggtaggggg tgcctcctcc ctggagaata ctgtggacct gcacatttcc aacagccacc
     1261 cactctccct cacctccgac cagtacaagg cctacctgca ggacttggtg gagggcatgg
     1321 atttccaggg ccctggggag agctgagccc tcgctcctca tggtgtgcct ccaacccccc
     1381 tgttccccac cacctcaacc aataaactgg ttcctgctat gaaaaaaaaa aaaaaaaana
     1441 aactcgagcg gccgctcgag catgcatcta gagggcccta ttctatagtg tcacctaaat
     1501 gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     1561 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     1621 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     1681 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     1741 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     1801 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     1861 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     1921 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     1981 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     2041 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     2101 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     2161 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     2221 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     2281 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     2341 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     2401 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     2461 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     2521 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     2581 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     2641 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     2701 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     2761 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     2821 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     2881 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     2941 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     3001 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     3061 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     3121 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     3181 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     3241 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     3301 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     3361 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     3421 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     3481 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     3541 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     3601 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     3661 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     3721 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     3781 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     3841 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     3901 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     3961 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     4021 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     4081 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4141 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4201 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4261 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4321 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4381 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4441 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4501 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4561 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4621 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4681 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4741 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4801 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4861 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     4921 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     4981 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     5041 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     5101 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     5161 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     5221 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     5281 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     5341 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     5401 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     5461 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     5521 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     5581 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     5641 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     5701 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     5761 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     5821 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     5881 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     5941 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     6001 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     6061 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     6121 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     6181 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     6241 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     6301 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     6361 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     6421 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     6481 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     6541 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     6601 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     6661 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     6721 cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg
     6781 cttatcgaaa ttaatacgac tcactatagg gagacccaag cttggtaccg agctcggatc
     6841 cactagtaac ggccgc
//
