LOCUS Exported 5397 bp ds-DNA circular SYN 17-JUN-2022 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS DsRed-rab11 WT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5397) AUTHORS Choudhury A, Dominguez M, Puri V, Sharma DK, Narita K, Wheatley CL, Marks DL, Pagano RE TITLE Rab proteins mediate Golgi transport of caveola-internalized glycosphingolipids and correct lipid trafficking in Niemann-Pick C cells. JOURNAL J Clin Invest. 2002 Jun . 109(12):1541-50. PUBMED 12070301 REFERENCE 2 (bases 1 to 5397) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 17, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5397 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 92..395 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 396..599 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 550..570 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 638..647 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 644..1321 /codon_start=1 /product="wild-type DsRed" /label=DsRed1 /note="mammalian codon-optimized" /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH HLFL" primer_bind complement(827..847) /label=DsRed1-N /note="DsRed1, reverse primer" primer_bind 1045..1064 /label=mCherry-F /note="mCherry, forward primer" primer_bind 1239..1262 /label=DsRed1-C /note="DsRed1, forward primer" misc_feature 1325..1381 /label=MCS /note="multiple cloning site" CDS 1385..1411 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 1412..1438 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" polyA_signal 2216..2337 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2253..2272) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2307..2326 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2344..2799) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2481..2502) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2693..2712 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2826..2930 /gene="bla" /label=AmpR promoter promoter 2932..3289 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2955..2975) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3140..3275 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3202..3221 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3324..4118 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3378..3397) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3988..4007 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4306..4325) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4350..4397 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4726..5314 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5215..5234 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ttctgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt 61 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc 121 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag 181 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc 241 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg 301 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc 361 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca 421 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 481 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg 541 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg 601 gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggtgc gctcctccaa 661 gaacgtcatc aaggagttca tgcgcttcaa ggtgcgcatg gagggcaccg tgaacggcca 721 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggccaca acaccgtgaa 781 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt 841 ccagtacggc tccaaggtgt acgtgaagca ccccgccgac atccccgact acaagaagct 901 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt 961 gaccgtgacc caggactcct ccctgcagga cggctgcttc atctacaagg tgaagttcat 1021 cggcgtgaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc 1081 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc acaaggccct 1141 gaagctgaag gacggcggcc actacctggt ggagttcaag tccatctaca tggccaagaa 1201 gcccgtgcag ctgcccggct actactacgt ggactccaag ctggacatca cctcccacaa 1261 cgaggactac accatcgtgg agcagtacga gcgcaccgag ggccgccacc acctgttcct 1321 gctcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc 1381 catgtaccca tacgatgttc cggattacgc ttacccatac gatgttccgg attacgctgg 1441 tacccgcgac gacgagtacg actacctctt taaagttgtc cttattggag attctggtgt 1501 tggaaagagt aatctcctgt ctcgatttac tcgaaatgag tttaatctgg aaagcaagag 1561 caccattgga gtagagtttg caacaagaag catccaggtt gatggaaaaa caataaaggc 1621 acagatatgg gacacagcag ggcaagagcg atatcgagct ataacatcag catattatcg 1681 tggagctgta ggtgccttat tggtttatga cattgctaaa catctcacat atgaaaatgt 1741 agagcgatgg ctgaaagaac tgagagatca tgctgatagt aacattgtta tcatgcttgt 1801 gggcaataag agtgatctac gtcatctcag ggcagttcct acagatgaag caagagcttt 1861 tgcagaaaag aatggtttgt cattcattga aacttcggcc ctagactcta caaatgtaga 1921 agctgctttt cagacaattt taacagagat ttaccgcatt gtttctcaga agcaaatgtc 1981 agacagacgc gaaaatgaca tgtctccaag caacaatgtg gttcctattc atgttccacc 2041 aaccactgaa aacaagccaa aggtgcagtg ctgtcagaac atctaaggat ccaccggatc 2101 tagataactg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa 2161 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt 2221 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 2281 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg 2341 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 2401 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 2461 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 2521 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 2581 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 2641 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 2701 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 2761 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa 2821 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 2881 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg 2941 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 3001 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 3061 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 3121 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 3181 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 3241 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag 3301 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 3361 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 3421 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 3481 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga 3541 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 3601 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 3661 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 3721 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 3781 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 3841 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 3901 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 3961 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 4021 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 4081 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat 4141 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 4201 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 4261 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga 4321 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg 4381 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac 4441 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc 4501 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat 4561 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 4621 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 4681 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 4741 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 4801 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 4861 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 4921 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 4981 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 5041 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 5101 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 5161 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 5221 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 5281 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 5341 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctga //