LOCUS       Exported                7333 bp ds-DNA     circular SYN 17-JUN-2022
DEFINITION  AAV packaging plasmid expressing Rep/Cap genes.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV2/7
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7333)
  TITLE     AAV packaging plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 17, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     17..39
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     31..48
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     32..48
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     105..121
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     106..122
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4114..4125
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(4577..4597)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4577..4595)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4616..4632)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4616..4632)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4629..4651)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4640..4656
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4664..4694)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4709..4730
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4847..4864)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5018..5606)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5098..5117)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5777..6637)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6400..6419
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6638..6742)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6768..7223)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6905..6926)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7117..7136
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa
       61 tacgactcac tatagggcga attgggtacc gggccccccc tcgatcgagg tcgacggtat
      121 cgggggagct cgcagggtct ccattttgaa gcgggaggtt tgaacgcgca gccgccatgc
      181 cggggtttta cgagattgtg attaaggtcc ccagcgacct tgacgagcat ctgcccggca
      241 tttctgacag ctttgtgaac tgggtggccg agaaggaatg ggagttgccg ccagattctg
      301 acatggatct gaatctgatt gagcaggcac ccctgaccgt ggccgagaag ctgcagcgcg
      361 actttctgac ggaatggcgc cgtgtgagta aggccccgga ggctcttttc tttgtgcaat
      421 ttgagaaggg agagagctac ttccacatgc acgtgctcgt ggaaaccacc ggggtgaaat
      481 ccatggtttt gggacgtttc ctgagtcaga ttcgcgaaaa actgattcag agaatttacc
      541 gcgggatcga gccgactttg ccaaactggt tcgcggtcac aaagaccaga aatggcgccg
      601 gaggcgggaa caaggtggtg gatgagtgct acatccccaa ttacttgctc cccaaaaccc
      661 agcctgagct ccagtgggcg tggactaata tggaacagta tttaagcgcc tgtttgaatc
      721 tcacggagcg taaacggttg gtggcgcagc atctgacgca cgtgtcgcag acgcaggagc
      781 agaacaaaga gaatcagaat cccaattctg atgcgccggt gatcagatca aaaacttcag
      841 ccaggtacat ggagctggtc gggtggctcg tggacaaggg gattacctcg gagaagcagt
      901 ggatccagga ggaccaggcc tcatacatct ccttcaatgc ggcctccaac tcgcggtccc
      961 aaatcaaggc tgccttggac aatgcgggaa agattatgag cctgactaaa accgcccccg
     1021 actacctggt gggccagcag cccgtggagg acatttccag caatcggatt tataaaattt
     1081 tggaactaaa cgggtacgat ccccaatatg cggcttccgt ctttctggga tgggccacga
     1141 aaaagttcgg caagaggaac accatctggc tgtttgggcc tgcaactacc gggaagacca
     1201 acatcgcgga ggccatagcc cacactgtgc ccttctacgg gtgcgtaaac tggaccaatg
     1261 agaactttcc cttcaacgac tgtgtcgaca agatggtgat ctggtgggag gaggggaaga
     1321 tgaccgccaa ggtcgtggag tcggccaaag ccattctcgg aggaagcaag gtgcgcgtgg
     1381 accagaaatg caagtcctcg gcccagatag acccgactcc cgtgatcgtc acctccaaca
     1441 ccaacatgtg cgccgtgatt gacgggaact caacgacctt cgaacaccag cagccgttgc
     1501 aagaccggat gttcaaattt gaactcaccc gccgtctgga tcatgacttt gggaaggtca
     1561 ccaagcagga agtcaaagac tttttccggt gggcaaagga tcacgtggtt gaggtggagc
     1621 atgaattcta cgtcaaaaag ggtggagcca agaaaagacc cgcccccagt gacgcagata
     1681 taagtgagcc caaacgggtg cgcgagtcag ttgcgcagcc atcgacgtca gacgcggaag
     1741 cttcgatcaa ctacgcagac aggtaccaaa acaaatgttc tcgtcacgtg ggcatgaatc
     1801 tgatgctgtt tccctgcaga caatgcgaga gaatgaatca gaattcaaat atctgcttca
     1861 ctcacggaca gaaagactgt ttagagtgct ttcccgtgtc agaatctcaa cccgtttctg
     1921 tcgtcaaaaa ggcgtatcag aaactgtgct acattcatca tatcatggga aaggtgccag
     1981 acgcttgcac tgcctgcgat ctggtcaatg tggatttgga tgactgcatc tttgaacaat
     2041 aaatgattta aatcaggtat ggctgccgat ggttatcttc cagattggct cgaggacaac
     2101 ctctctgagg gcattcgcga gtggtgggac ctgaaacctg gagccccgaa acccaaagcc
     2161 aaccagcaaa agcaggacaa cggccggggt ctggtgcttc ctggctacaa gtacctcgga
     2221 cccttcaacg gactcgacaa gggggagccc gtcaacgcgg cggacgcagc ggccctcgag
     2281 cacgacaagg cctacgacca gcagctcaaa gcgggtgaca atccgtacct gcggtataac
     2341 cacgccgacg ccgagtttca ggagcgtctg caagaagata cgtcatttgg gggcaacctc
     2401 gggcgagcag tcttccaggc caagaagcgg gttctcgaac ctctcggtct ggttgaggaa
     2461 ggcgctaaga cggctcctgc aaagaagaga ccggtagagc cgtcacctca gcgttccccc
     2521 gactcctcca cgggcatcgg caagaaaggc cagcagcccg ccagaaagag actcaatttc
     2581 ggtcagactg gcgactcaga gtcagtcccc gaccctcaac ctctcggaga acctccagca
     2641 gcgccctcta gtgtgggatc tggtacagtg gctgcaggcg gtggcgcacc aatggcagac
     2701 aataacgaag gtgccgacgg agtgggtaat gcctcaggaa attggcattg cgattccaca
     2761 tggctgggcg acagagtcat taccaccagc acccgaacct gggccctgcc cacctacaac
     2821 aaccacctct acaagcaaat ctccagtgaa actgcaggta gtaccaacga caacacctac
     2881 ttcggctaca gcaccccctg ggggtatttt gactttaaca gattccactg ccacttctca
     2941 ccacgtgact ggcagcgact catcaacaac aactggggat tccggcccaa gaagctgcgg
     3001 ttcaagctct tcaacatcca ggtcaaggag gtcacgacga atgacggcgt tacgaccatc
     3061 gctaataacc ttaccagcac gattcaggta ttctcggact cggaatacca gctgccgtac
     3121 gtcctcggct ctgcgcacca gggctgcctg cctccgttcc cggcggacgt cttcatgatt
     3181 cctcagtacg gctacctgac tctcaacaat ggcagtcagt ctgtgggacg ttcctccttc
     3241 tactgcctgg agtacttccc ctctcagatg ctgagaacgg gcaacaactt tgagttcagc
     3301 tacagcttcg aggacgtgcc tttccacagc agctacgcac acagccagag cctggaccgg
     3361 ctgatgaatc ccctcatcga ccagtacttg tactacctgg ccagaacaca gagtaaccca
     3421 ggaggcacag ctggcaatcg ggaactgcag ttttaccagg gcgggccttc aactatggcc
     3481 gaacaagcca agaattggtt acctggacct tgcttccggc aacaaagagt ctccaaaacg
     3541 ctggatcaaa acaacaacag caactttgct tggactggtg ccaccaaata tcacctgaac
     3601 ggcagaaact cgttggttaa tcccggcgtc gccatggcaa ctcacaagga cgacgaggac
     3661 cgctttttcc catccagcgg agtcctgatt tttggaaaaa ctggagcaac taacaaaact
     3721 acattggaaa atgtgttaat gacaaatgaa gaagaaattc gtcctactaa tcctgtagcc
     3781 acggaagaat acgggatagt cagcagcaac ttacaagcgg ctaatactgc agcccagaca
     3841 caagttgtca acaaccaggg agccttacct ggcatggtct ggcagaaccg ggacgtgtac
     3901 ctgcagggtc ccatctgggc caagattcct cacacggatg gcaactttca cccgtctcct
     3961 ttgatgggcg gctttggact taaacatccg cctcctcaga tcctgatcaa gaacactccc
     4021 gttcccgcta atcctccgga ggtgtttact cctgccaagt ttgcttcgtt catcacacag
     4081 tacagcaccg gacaagtcag cgtggaaatc gagtgggagc tgcagaagga aaacagcaag
     4141 cgctggaacc cggagattca gtacacctcc aactttgaaa agcagactgg tgtggacttt
     4201 gccgttgaca gccagggtgt ttactctgag cctcgcccta ttggcactcg ttacctcacc
     4261 cgtaatctgt aattgcatgt taatcaataa accggttgat tcgtttcagt tgaactttgg
     4321 tctctgcgaa gggcgaattc gtttaaacct gcaggactag aggtcctgta ttagaggtca
     4381 cgtgagtgtt ttgcgacatt ttgcgacacc atgtggtcac gctgggtatt taagcccgag
     4441 tgagcacgca gggtctccat tttgaagcgg gaggtttgaa cgcgcagccg ccaagccgaa
     4501 ttctgcagat atccatcaca ctggcggccg ctcgactaga gcggccgcca ccgcggtgga
     4561 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     4621 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     4681 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     4741 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     4801 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     4861 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4921 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4981 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5041 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5101 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5161 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5221 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5281 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5341 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5401 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     5461 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5521 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5581 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5641 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5701 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     5761 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5821 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5881 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5941 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6001 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6061 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6121 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6181 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6241 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     6301 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     6361 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     6421 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     6481 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     6541 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6601 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     6661 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     6721 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt
     6781 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     6841 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     6901 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     6961 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     7021 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     7081 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     7141 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     7201 taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg
     7261 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     7321 gcgattaagt tgg
//
