LOCUS Exported 3992 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pMtBS SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3992) AUTHORS Schowalter RM, Pastrana DV, Buck CB TITLE Glycosaminoglycans and sialylated glycans sequentially facilitate merkel cell polyomavirus infectious entry. JOURNAL PLoS Pathog. 2011 Jul;7(7):e1002161. Epub 2011 Jul 28. PUBMED 21829355 REFERENCE 2 (bases 1 to 3992) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3992 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(74..94) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" intron 449..1396 /label=mEF-1-alpha intron /note="intron upstream of the start codon of mouse EF-1-alpha" misc_feature 2028..2472 /label=FMDV IRES /note="internal ribosome entry site (IRES) of the foot-and-mouth disease virus" promoter 2506..2552 /label=EM7 promoter /note="synthetic bacterial promoter " promoter 2544..2562 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal 3024..3145 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3035..3054) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 3089..3108 /label=SV40pA-R /note="SV40 polyA, reverse primer" rep_origin 3333..3921 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3822..3841 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggctga 61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca 121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 241 ccactagttc cgccagagcg cgcgagggcc tccagcggcc gcccctcccc cacagcaggg 301 gcggggtccc gcgcccaccg gaaggagcgg gctcggggcg ggcggcgctg attggccggg 361 gcgggcctga cgccgacgcg gctataagag accacaagcg acccgcaggg ccagacgttc 421 ttcgccgaag cttgccgtca gaacgcaggt gaggggcggg tgtggcttcc gcgggccgcc 481 gagctggagg tcctgctccg agcgggccgg gccccgctgt cgtcggcggg gattagctgc 541 gagcattccc gcttcgagtt gcgggcggcg cgggaggcag agtgcgaggc ctagcggcaa 601 ccccgtagcc tcgcctcgtg tccggcttga ggcctagcgt ggtgtccgcg ccgccgccgc 661 gtgctactcc ggccgcactc tggtcttttt tttttttgtt gttgttgccc tgctgccttc 721 gattgccgtt cagcaatagg ggctaacaaa gggagggtgc ggggcttgct cgcccggagc 781 ccggagaggt catggttggg gaggaatgga gggacaggag tggcggctgg ggcccgcccg 841 ccttcggagc acatgtccga cgccacctgg atggggcgag gcctggggtt tttcccgaag 901 caaccaggct ggggttagcg tgccgaggcc atgtggcccc agcacccggc acgatctggc 961 ttggcggcgc cgcgttgccc tgcctcccta actagggtga ggccatcccg tccggcacca 1021 gttgcgtgcg tggaaagatg gccgctcccg ggccctgttg caaggagctc aaaatggagg 1081 acgcggcagc ccggtggagc gggcgggtga gtcacccaca caaaggaaga gggcctggtc 1141 cctcaccggc tgctgcttcc tgtgaccccg tggtcctatc ggccgcaata gtcacctcgg 1201 gcttttgagc acggctagtc gcggcggggg gaggggatgt aatggcgttg gagtttgttc 1261 acatttggtg ggtggagact agtcaggcca gcctggcgct ggaagtcatt tttggaattt 1321 gtccccttga gttttgagcg gagctaattc tcgggcttct tagcggttca aaggtatctt 1381 ttaaaccctt ttttaggtgt tgtgaaaacc accgctaatt caaagcaacc ggtgatatcg 1441 gatccacgcg taagatggat ttagtcctaa ataggaaaga aagagaggct ctctgcaagc 1501 ttttagagat tgctcctaat tgttatggca acatccctct gatgaaagct gctttcaaaa 1561 gaagctgctt aaagcatcac cctgataaag ggggaaatcc tgttataatg atggaattga 1621 acaccctttg gagcaaattc cagcaaaata tccacaagct cagaagtgac ttctctatgt 1681 ttgatgaagt ctccacaaaa tttccatggg aagaatatgg aactttaaag gattatatgc 1741 aaagtggata taatgctaga ttttgcagag gtcctgggtg catgcttaag caacttagag 1801 attctaagtg cgcttgtatt agctgtaagt tgtctcgcca gcattgtagt ctaaaaactt 1861 taaagcaaaa aaactgtctg acgtggggag agtgtttttg ctatcagtgc tttattcttt 1921 ggtttggatt tcctcctact tgggaaagtt ttgactggtg gcaaaaaact ttagaagaaa 1981 ctgactactg cttactgcat ctgcaccttt tctgagtcga ccctaggagc aggtttcccc 2041 aatgacacaa aacgtgcaac ttgaaactcc gcctggtctt tccaggtcta gaggggtaac 2101 actttgtact gcgtttggct ccacgctcga tccactggcg agtgttagta acagcactgt 2161 tgcttcgtag cggagcatga cggccgtggg aactcctcct tggtaacaag gacccacggg 2221 gccaaaagcc acgcccacac gggcccgtca tgtgtgcaac cccagcacgg cgactttact 2281 gcgaaaccca ctttaaagtg acattgaaac tggtacccac acactggtga caggctaagg 2341 atgcccttca ggtaccccga ggtaacacgc gacactcggg atctgagaag gggactgggg 2401 cttctataaa agcgctcggt ttaaaaagct tctatgcctg aataggtgac cggaggtcgg 2461 cacctttcct ttgcaattac tgaccctatg aatacaactg actgtttgac aattaatcat 2521 cggcatagta tatcggcata gtataatacg actcactata ggagggccac catgaagacc 2581 ttcaacatct ctcagcagga tctggagctg gtggaggtcg ccactgagaa gatcaccatg 2641 ctctatgagg acaacaagca ccatgtcggg gcggccatca ggaccaagac tggggagatc 2701 atctctgctg tccacattga ggcctacatt ggcagggtca ctgtctgtgc tgaagccatt 2761 gccattgggt ctgctgtgag caacgggcag aaggactttg acaccattgt ggctgtcagg 2821 cacccctact ctgatgaggt ggacagatcc atcagggtgg tcagcccctg tggcatgtgc 2881 agagagctca tctctgacta tgctcctgac tgctttgtgc tcattgagat gaatggcaag 2941 ctggtcaaaa ccaccattga ggaactcatc cccctcaagt acaccaggaa ctaaacctga 3001 attcgctagc tggccagaca tgataagata cattgatgag tttggacaaa ccacaactag 3061 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac 3121 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt 3181 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatgga 3241 aatgttaatt aactagccat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 3301 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 3361 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 3421 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt 3481 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 3541 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 3601 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 3661 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 3721 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 3781 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 3841 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 3901 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 3961 tgctggcctt ttgctcacat gttcttaatt aa //