LOCUS Exported 6604 bp ds-DNA circular SYN 28-JUN-2022 DEFINITION Localization: Kinetochore, Excitation: 505 / 569, Emission: 516 / 581. ACCESSION . VERSION . KEYWORDS mEos2-Hec1-C-12 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6604) AUTHORS McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL TITLE A bright and photostable photoconvertible fluorescent protein. JOURNAL Nat Methods. 2009 Feb . 6(2):131-3. PUBMED 19169260 REFERENCE 2 (bases 1 to 6604) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 28, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6604 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 2530..3210 /codon_start=1 /product="monomeric variant of green-to-red photoswitchable fluorescent protein EosFP (McKinney et al., 2009)" /label=mEos2 /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD NARR" polyA_signal 3332..3453 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3369..3388) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3423..3442 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(3460..3915) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3597..3618) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3809..3828 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3942..4046 /gene="bla" /label=AmpR promoter promoter 4048..4405 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(4071..4091) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 4256..4391 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4318..4337 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4440..5234 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4494..4513) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 5104..5123 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(5422..5441) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 5466..5513 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5842..6430 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6331..6350 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 541 ccggactcag atctcgagct cgccaccatg aagcgcagtt cagtttccag cggtggtgct 601 ggccgcctct ccatgcagga gttaagatcc caggatgtaa ataaacaagg cctctatacc 661 cctcaaacca aagagaaacc aacctttgga aagttgagta taaacaaacc gacatctgaa 721 agaaaagtct cgctatttgg caaaagaact agtggacatg gatcacggaa tagtcaactt 781 ggtatatttt ccagttctga gaaaatcaag gacccgagac cacttaacga caaagcattc 841 attcagcagt gtattcgaca actctgtgag tttcttacag aaaatggtta tgcacataat 901 gtgtccatga aatctctaca agctccctct gttaaagact tcctgaagat cttcacattt 961 ctttatggct tcctgtgccc ctcatacgaa cttcctgaca caaagtttga agaagaggtt 1021 ccaagaatct ttaaagacct tgggtatcct tttgcactat ccaaaagctc catgtacaca 1081 gtgggggctc ctcatacatg gcctcacatt gtggcagcct tagtttggct aatagactgc 1141 atcaagatac atactgccat gaaagaaagc tcacctttat ttgatgatgg gcagccttgg 1201 ggagaagaaa ctgaagatgg aattatgcat aataagttgt ttttggacta caccataaaa 1261 tgctatgaga gttttatgag tggtgccgac agctttgatg agatgaatgc agagctgcag 1321 tcaaaactga aggatttatt taatgtggat gcttttaagc tggaatcatt agaagcaaaa 1381 aacagagcat tgaatgaaca gattgcaaga ttggaacaag aaagagaaaa agaaccgaat 1441 cgtctagagt cgttgagaaa actgaaggct tccttacaag gagatgttca aaagtatcag 1501 gcatacatga gcaatttgga gtctcattca gccattcttg accagaaatt aaatggtctc 1561 aatgaggaaa ttgctagagt agaactagaa tgtgaaacaa taaaacagga gaacactcga 1621 ctacagaata tcattgacaa ccagaagtac tcagttgcag acattgagcg aataaatcat 1681 gaaagaaatg aattgcagca gactattaat aaattaacca aggacctgga agctgaacaa 1741 cagaagttgt ggaatgagga gttaaaatat gccagaggca aagaagcgat tgaaacacaa 1801 ttagcagagt atcacaaatt ggctagaaaa ttaaaactta ttcctaaagg tgctgagaat 1861 tccaaaggtt atgactttga aattaagttt aatcccgagg ctggtgccaa ctgccttgtc 1921 aaatacaggg ctcaagttta tgtacctctt aaggaactcc tgaatgaaac tgaagaagaa 1981 attaataaag ccctaaataa aaaaatgggt ttggaggata ctttagaaca attgaatgca 2041 atgataacag aaagcaagag aagtgtgaga actctgaaag aagaagttca aaagctggat 2101 gatctttacc aacaaaaaat taaggaagca gaggaagagg atgaaaaatg tgccagtgag 2161 cttgagtcct tggagaaaca caagcacctg ctagaaagta ctgttaacca ggggctcagt 2221 gaagctatga atgaattaga tgctgttcag cgggaatacc aactagttgt gcaaaccacg 2281 actgaagaaa gacgaaaagt gggaaataac ttgcaacgtc tgttagagat ggttgctaca 2341 catgttgggt ctgtagagaa acatcttgag gagcagattg ctaaagttga tagagaatat 2401 gaagaatgca tgtcagaaga tctctcggaa aatattaaag agattagaga taagtatgag 2461 aagaaagcta ctctaattaa gtcttctgaa gaagcggtac cgcgggcccg ggatccaccg 2521 gtcgccacca tgagtgcgat taagccagac atgaagatca aactccgtat ggaaggcaac 2581 gtaaacgggc accactttgt gatcgacgga gatggtacag gcaagccttt tgagggaaaa 2641 cagagtatgg atcttgaagt caaagagggc ggacctctgc cttttgcctt tgatatcctg 2701 accactgcat tccattacgg caacagggta ttcgccaaat atccagacaa catacaagac 2761 tattttaagc agtcgtttcc taaggggtat tcgtgggaac gaagcttgac tttcgaagac 2821 gggggcattt gcattgccag aaacgacata acaatggaag gggacacttt ctataataaa 2881 gttcgatttt atggtaccaa ctttcccgcc aatggtccag ttatgcagaa gaagacgctg 2941 aaatgggagc cctccactga gaaaatgtat gtgcgtgatg gagtgctgac gggtgatatt 3001 catatggctt tgttgcttga aggaaatgcc cattaccgat gtgacttcag aactacttac 3061 aaagctaagg agaagggtgt caagttacca ggctaccact ttgtggacca ctgcattgag 3121 attttaagcc atgacaaaga ttacaacaag gttaagctgt atgagcatgc tgttgctcat 3181 tctggattgc ctgacaatgc cagacgataa gcggccgcga ctctagatca taatcagcca 3241 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct 3301 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta 3361 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 3421 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt 3481 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa 3541 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag 3601 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg 3661 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga 3721 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg 3781 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg 3841 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc 3901 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 3961 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 4021 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg 4081 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 4141 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 4201 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc 4261 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 4321 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 4381 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca 4441 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 4501 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 4561 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 4621 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 4681 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 4741 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 4801 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 4861 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 4921 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg 4981 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 5041 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 5101 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 5161 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 5221 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct 5281 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 5341 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 5401 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc 5461 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg 5521 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca 5581 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg 5641 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact 5701 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 5761 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 5821 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 5881 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 5941 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 6001 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 6061 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 6121 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 6181 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 6241 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 6301 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 6361 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 6421 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 6481 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat 6541 gcattagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt 6601 tccg //