LOCUS Exported 3597 bp ds-DNA circular SYN 29-JUN-2022 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence. . ACCESSION . VERSION . KEYWORDS pDONR221-SLC10A2_STOP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3597) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 3597) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 29, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3597 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1154..1253) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1270..1289) /label=T7 /note="T7 promoter, forward primer" promoter complement(1271..1289) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1294..1310) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1294..1310) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1412..1431) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1423..2232 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1490..1509) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2379..2967 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2868..2887 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3121..3138 /label=L4440 /note="L4440 vector, forward primer" terminator 3297..3324 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3416..3502 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3551..3573 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3565..3582 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3566..3582 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgaa cgatcccaat 121 tcttgcgtgg acaacgcaac cgtgtgcagc ggagcatcct gcgtggtgcc tgagagcaat 181 ttcaacaata tcctgtccgt ggtgctgtct accgtgctga caatcctgct ggccctggtc 241 atgttctcca tgggctgtaa cgtggagatc aagaagtttc tgggccacat caagaggcct 301 tggggcatct gcgtgggctt cctgtgccag tttggcatca tgccactgac cggcttcatc 361 ctgtctgtgg cctttgatat cctgccactg caggccgtgg tggtgctgat catcggatgc 421 tgtccaggag gaacagcaag caatatcctg gcctactggg tggacggcga tatggacctg 481 tctgtgagca tgaccacatg ctccaccctg ctggccctgg gcatgatgcc actgtgcctg 541 ctgatctaca caaagatgtg ggtggattcc ggctctatcg tgatccccta tgacaacatc 601 ggcacctctc tggtgagcct ggtggtgccc gtgagcatcg gcatgtttgt gaatcacaag 661 tggcctcaga aggccaagat catcctgaag atcggctcca tcgccggcgc catcctgatc 721 gtgctgatcg ccgtggtggg cggcatcctg tatcagtctg cctggatcat cgccccaaag 781 ctgtggatca tcggcacaat cttccccgtg gccggctact ccctgggctt tctgctggca 841 aggatcgcag gactgccttg gtatcggtgc agaaccgtgg ccttcgagac aggcatgcag 901 aacacccagc tgtgcagcac aatcgtgcag ctgtccttta cccctgagga gctgaatgtg 961 gtgttcacat ttccactgat ctactctatc ttccagctgg cctttgccgc catcttcctg 1021 ggcttttacg tggcctataa gaagtgccac ggcaagaaca aggccgagat ccccgagagc 1081 aaggagaatg gcaccgagcc tgagagctcc ttctataagg ccaacggcgg ctttcagccc 1141 gacgagaagt aagacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta 1201 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca 1261 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg 1321 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca 1381 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg 1441 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg 1501 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat 1561 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag 1621 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc 1681 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag 1741 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg 1801 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt 1861 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac 1921 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc 1981 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag 2041 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat 2101 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt 2161 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat 2221 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact 2281 tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac 2341 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 2401 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 2461 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 2521 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 2581 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 2641 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 2701 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 2761 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 2821 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 2881 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 2941 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 3001 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 3061 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 3121 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 3181 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 3241 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa 3301 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc 3361 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg 3421 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg 3481 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca 3541 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccc //