LOCUS Exported 5939 bp ds-DNA circular SYN 30-JUN-2022 DEFINITION For expression of recombinant Lck in SF9 cells.. ACCESSION . VERSION . KEYWORDS Lck SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5939) AUTHORS Case LB, De Pasquale M, Henry L, Rosen MK TITLE Synergistic phase separation of two pathways promotes integrin clustering and nascent adhesion formation. JOURNAL Elife. 2022 Jan 20;11. pii: 72588. doi: 10.7554/eLife.72588. PUBMED 35049497 REFERENCE 2 (bases 1 to 5939) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 30, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5939 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 375..394 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 628..645 /label=L4440 /note="L4440 vector, forward primer" mobile_element 779..1003 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (right end of the Tn7 transposon)" promoter complement(1773..1801) /gene="intI1 (promoter lies within the coding sequence)" /label=Pc promoter /note="class 1 integron promoter" promoter 2153..2244 /gene="polh from Autographa californica" /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" primer_bind 2169..2186 /label=Polyhedrin-F /note="Polyhedrin promoter, forward primer" CDS 2311..2328 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 2350..2370 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" polyA_signal 3697..3831 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3734..3753) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3788..3807 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" mobile_element 3860..4025 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (left end of the Tn7 transposon)" rep_origin 4209..4663 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4296..4315) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4505..4526 /label=F1ori-F /note="F1 origin, forward primer" promoter 4689..4793 /gene="bla" /label=AmpR promoter CDS 4794..5654 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5012..5031) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5825..474 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 61 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 121 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 181 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 241 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 301 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 361 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 421 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 481 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 541 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 601 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 661 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat agaccagccg 721 cgtaacctgg caaaatcggt tacggttgag taataaatgg atgccctgcg taagcgggtg 781 tgggcggaca ataaagtctt aaactgaaca aaatagatct aaactatgac aataaagtct 841 taaactagac agaatagttg taaactgaaa tcagtccagt tatgctgtga aaaagcatac 901 tggacttttg ttatggctaa agcaaactct tcattttctg aagtgcaaat tgcccgtcgt 961 attaaagagg ggcgtggcca agggcatggt aaagactata ttcgcggcgt tgtgacaatt 1021 taccgaacaa ctccgcggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg 1081 tacttgggtc gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag 1141 ccactgcggg atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt 1201 tggcctcatg cttgaggaga ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc 1261 cggagactgc gagatcatag atatagatct cactacgcgg ctgctcaaac ttgggcagaa 1321 cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg 1381 tcttactacg gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg 1441 ctacgtctcc gaactcacga ccgaaaagat caagagcagc ccgcatggat ttgacttggt 1501 cagggccgag cctacatgtg cgaatgatgc ccatacttga gccacctaac tttgttttag 1561 ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg 1621 cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac 1681 aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc 1741 agttgcgtga gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca 1801 actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca 1861 gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg gtctccacgc 1921 atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc acggatctgc 1981 cctggcttca ggagatcgga agacctcggc cgtcgcggcg cttgccggtg gtgctgaccc 2041 cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg ttcgcccagg 2101 actctagcta tagttctagt ggttggctac gtatactccg gaatattaat agatcatgga 2161 gataattaaa atgataacca tctcgcaaat aaataagtat tttactgttt tcgtaacagt 2221 tttgtaataa aaaaacctat aaatattccg gattattcat accgtcccac catcgggcgc 2281 ggatctcggt ccgaaaccat gtcgtactac catcaccatc accatcacga ttacgatatc 2341 ccaacgaccg aaaacctgta ttttcagggc gccatggatc cggaattcgc aaacagcctg 2401 gagcctgaac cttggttctt caagaatctg agccgtaagg acgccgagcg gcagcttttg 2461 gcgcccggga acacgcatgg atccttcctg atccgggaaa gcgaaagcac tgcggggtcc 2521 ttttccctgt cggtcagaga cttcgaccag aaccagggag aagtggtgaa acattacaag 2581 atccgtaacc tagacaacgg tggcttctac atctcccctc gtatcacttt tcccggattg 2641 cacgatctag tccgccatta caccaacgcc tctgatgggc tgtgcacaaa gttgagccgt 2701 ccttgccaga cccagaagcc ccagaaacca tggtgggagg acgaatggga agttcccagg 2761 gaaacactga agttggtgga gcggctggga gctggccagt tcggggaagt gtggatgggg 2821 tactacaacg gacacacgaa ggtggcggtg aagagtctga aacaagggag catgtccccc 2881 gacgccttcc tggctgaggc taacctcatg aagcagctgc agcacccgcg gctagtccgg 2941 ctttatgcag tggtcaccca ggaacccatc tacatcatca cggaatacat ggagaacggg 3001 agcctagtag attttctcaa gactccctcg ggcatcaagt tgaatgtcaa caaacttttg 3061 gacatggcag cccagattgc agagggcatg gcgttcatcg aagaacagaa ttacatccat 3121 cgggacctgc gcgccgccaa catcctggtg tctgacacgc tgagctgcaa gattgcagac 3181 tttggcctgg cgcgcctcat tgaggacaat gagtacacgg cccgggaggg ggccaaattt 3241 cccattaagt ggacagcacc agaagccatt aactatggga ccttcaccat caagtcagac 3301 gtgtggtcct tcgggatctt gcttacagag atcgtcaccc acggtcgaat cccttaccca 3361 ggaatgacca accctgaagt cattcagaac ctggagagag gctaccgcat ggtgagacct 3421 gacaactgtc cggaagagct gtaccacctc atgatgctgt gctggaagga gcgcccagag 3481 gaccggccca cgtttgacta ccttcggagt gttctggatg acttcttcac agccacagag 3541 ggccagttcc agccccagcc ttgatagaag cttgtcgaga agtactagag gatcataatc 3601 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg 3661 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat 3721 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 3781 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg 3841 cttgagccta ggagatccga accagataag tgaaatctag ttccaaacta ttttgtcatt 3901 tttaattttc gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc 3961 cctaaataat ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc 4021 ccacagcggg gcatttttct tcctgttatg tttttaatca aacatcctgc caactccatg 4081 tgacaaaccg tcatcttcgg ctactttttc tctgtcacag aatgaaaatt tttctgtcat 4141 ctcttcgtta ttaatgtttg taattgactg aatatcaacg cttatttgca gcctgaatgg 4201 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 4261 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 4321 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 4381 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 4441 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 4501 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt 4561 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa 4621 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg 4681 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 4741 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 4801 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 4861 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 4921 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 4981 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 5041 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 5101 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 5161 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 5221 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 5281 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 5341 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 5401 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 5461 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 5521 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 5581 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 5641 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 5701 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 5761 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 5821 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 5881 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaact //