LOCUS       Exported                3552 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pENTR Bmi1-C
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Pasque V, Gillich A, Garrett N, Gurdon JB
  TITLE     Histone variant macroH2A confers resistance to nuclear 
            reprogramming.
  JOURNAL   EMBO J. 2011 May 6. ():.
  PUBMED    21552206
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(17..116)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(133..152)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(134..152)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(157..173)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(157..173)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(275..294)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             286..1095
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(353..372)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1731..1750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1984..2001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      2160..2187
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      2279..2365
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2414..2436
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2428..2445
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2429..2445
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2461..2560
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 aagggtgggc gcgccgaccc agctttcttg tacaaagttg gcattataag aaagcattgc
       61 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
      121 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
      181 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
      241 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
      301 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
      361 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
      421 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
      481 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
      541 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
      601 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
      661 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
      721 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
      781 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
      841 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
      901 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
      961 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     1021 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     1081 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     1141 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
     2581 atgcatcgaa caaccagaat caagatcact gagctaaatc cccacttaat gtgtgtcctg
     2641 tgtggagggt acttcattga tgccactacc ataatagaat gtctacattc cttctgtaaa
     2701 acatgtattg tacgttactt ggagaccagc aagtattgtc ctatttgtga tgtccaggtt
     2761 cacaaaacca gaccactcct gaacataagg tcagataaaa ctcttcaaga tattgtatac
     2821 aaattagtcc cagggctttt caaaaatgag atgaagagaa gaagagattt ttatgcagct
     2881 cacccgtcag ctgatgctgc caatggctcc aatgaagacc gaggagaagt tgcagatgag
     2941 gagaagagga ttataactga tgatgagata ataagcttgt ctattgagtt ctttgatcag
     3001 agcagattgg atcggaaagt aaataaagag aagcctaagg aagaggtgaa tgataaaagg
     3061 tacttacgat gcccagcagc aatgactgtg atgcacttga gaaagtttct caggagtaaa
     3121 atggacatac ccaatacttt ccagattgac gtcatgtatg aagaggaacc tttaaaggat
     3181 tactacacgc taatggacat tgcctacatt tatacctgga gaagaaatgg cccactacct
     3241 ttgaaataca gagttcggcc aacttgcaaa agaatgaaga tgagtcacca gagggatgga
     3301 ctgacgaatg ctggagagct ggaaagtgac tctgggagtg acaaggccaa cagcccagcc
     3361 ggaggtgttc cctccacctc ttcctgtttg cccagtccca gcactccagt gcagtctcct
     3421 cacccacagt tccctcacat ttccagtacc atgaatggaa ccagcaacag ccccagtgct
     3481 aaccaccaat cttcctttgc cagtagacct cgaaaatcat cactaaatgg gtcatcagca
     3541 acttcatctg gt
//
