LOCUS Exported 15095 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS hATMS1981A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 15095) AUTHORS Bakkenist CJ, Kastan MB TITLE DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation. JOURNAL Nature. 2003 Jan 30;421(6922):499-506. PUBMED 12556884 REFERENCE 2 (bases 1 to 15095) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..15095 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 998..1021 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 1025..1042 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" promoter complement(10651..10669) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(10652..10669) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(10689..10706) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 10695..10919 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 10965..11393 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(11052..11071) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 11262..11283 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(11402..11422) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 11407..11736 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 11587..11722 /label=SV40 ori /note="SV40 origin of replication" primer_bind 11649..11668 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 11803..12597 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(11857..11876) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 12467..12486 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 12771..12892 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(12808..12827) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 12862..12881 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(12941..12957) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(12941..12957) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(12954..12976) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 12965..12981 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(12989..13019) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 13034..13055 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(13172..13189) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(13343..13928) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(13423..13442) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(14099..14959) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 14722..14741 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(14960..15064) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc 961 agatatccat cacactggcg gccgctcgac caccatggac tacaaggacg atgacgacaa 1021 gatgcatcat catcatcatc atatgagtct agtacttaat gatctgctta tctgctgccg 1081 tcaactagaa catgatagag ctacagaacg aaagaaagaa gttgagaaat ttaagcgcct 1141 gattcgagat cctgaaacaa ttaaacatct agatcggcat tcagattcca aacaaggaaa 1201 atatttgaat tgggatgctg tttttagatt tttacagaaa tatattcaga aagaaacaga 1261 atgtctgaga atagcaaaac caaatgtatc agcctcaaca caagcctcca ggcagaaaaa 1321 gatgcaggaa atcagtagtt tggtcaaata cttcatcaaa tgtgcaaaca gaagagcacc 1381 taggctaaaa tgtcaagaac tcttaaatta tatcatggat acagtgaaag attcatctaa 1441 tggtgctatt tacggagctg attgtagcaa catactactc aaagacattc tttctgtgag 1501 aaaatactgg tgtgaaatat ctcagcaaca gtggttagaa ttgttctctg tgtacttcag 1561 gctctatctg aaaccttcac aagatgttca tagagtttta gtggctagaa taattcatgc 1621 tgttaccaaa ggatgctgtt ctcagactga cggattaaat tccaaatttt tggacttttt 1681 ttccaaggct attcagtgtg cgagacaaga aaagagctct tcaggtctaa atcatatctt 1741 agcagctctt actatcttcc tcaagacttt ggctgtcaac tttcgaattc gagtgtgtga 1801 attaggagat gaaattcttc ccactttgct ttatatttgg actcaacata ggcttaatga 1861 ttctttaaaa gaagtcatta ttgaattatt tcaactgcaa atttatatcc atcatccgaa 1921 aggagccaaa acccaagaaa aaggtgctta tgaatcaaca aaatggagaa gtattttata 1981 caacttatat gatctgctag tgaatgagat aagtcatata ggaagtagag gaaagtattc 2041 ttcaggattt cgtaatattg ccgtcaaaga aaatttgatt gaattgatgg cagatatctg 2101 tcaccaggtt tttaatgaag ataccagatc cttggagatt tctcaatctt acactactac 2161 acaaagagaa tctagtgatt acagtgtccc ttgcaaaagg aagaaaatag aactaggctg 2221 ggaagtaata aaagatcacc ttcagaagtc acagaatgat tttgatcttg tgccttggct 2281 acagattgca acccaattaa tatcaaagta tcctgcaagt ttacctaact gtgagctgtc 2341 tccattactg atgatactat ctcagcttct accccaacag cgacatgggg aacgtacacc 2401 atatgtgtta cgatgcctta cggaagttgc attgtgtcaa gacaagaggt caaacctaga 2461 aagctcacaa aagtcagatt tattaaaact ctggaataaa atttggtgta ttacctttcg 2521 tggtataagt tctgagcaaa tacaagctga aaactttggc ttacttggag ccataattca 2581 gggtagttta gttgaggttg acagagaatt ctggaagtta tttactgggt cagcctgcag 2641 accttcatgt cctgcagtat gctgtttgac tttggcactg accaccagta tagttccagg 2701 agcggtaaaa atgggaatag agcaaaatat gtgtgaagta aatagaagct tttctttaaa 2761 ggaatcaata atgaaatggc tcttattcta tcagttagag ggtgacttag aaaatagcac 2821 agaagtgcct ccaattcttc acagtaattt tcctcatctt gtactggaga aaattcttgt 2881 gagtctcact atgaaaaact gtaaagctgc aatgaatttt ttccaaagcg tgccagaatg 2941 tgaacaccac caaaaagata aagaagaact ttcattctca gaagtagaag aactatttct 3001 tcagacaact tttgacaaga tggacttttt aaccattgtg agagaatgtg gtatagaaaa 3061 gcaccagtcc agtattggct tctctgtcca ccagaatctc aaggaatcac tggatcgctg 3121 tcttctggga ttatcagaac agcttctgaa taattactca tctgagatta caaattcaga 3181 aactcttgtc cggtgttcac gtcttttggt gggtgtcctt ggctgctact gttacatggg 3241 tgtaatagct gaagaggaag catataagtc agaattattc cagaaagcca actctctaat 3301 gcaatgtgca ggagaaagta tcactctgtt taaaaataag acaaatgagg aattcagaat 3361 tggttccttg agaaatatga tgcagctatg tacacgttgc ttgagcaact gtaccaagaa 3421 gagtccaaat aagattgcat ctggcttttt cctgcgattg ttaacatcaa agctaatgaa 3481 tgacattgca gatatttgta aaagtttagc atccttcatc aaaaagccat ttgaccgtgg 3541 agaagtagaa tcaatggaag atgatactaa tggaaatcta atggaggtgg aggatcagtc 3601 atccatgaat ctatttaacg attaccctga tagtagtgtt agtgatgcaa acgaacctgg 3661 agagagccaa agtaccatag gtgccattaa tcctttagct gaagaatatc tgtcaaagca 3721 agatctactt ttcttagaca tgctcaagtt cttgtgtttg tgtgtaacta ctgctcagac 3781 caatactgtg tcctttaggg cagctgatat tcggaggaaa ttgttaatgt taattgattc 3841 tagcacgcta gaacctacca aatccctcca cctgcatatg tatctaatgc ttttaaagga 3901 gcttcctgga gaagagtacc ccttgccaat ggaagatgtt cttgaacttc tgaaaccact 3961 atccaatgtg tgttctttgt atcgtcgtga ccaagatgtt tgtaaaacta ttttaaacca 4021 tgtccttcat gtagtgaaaa acctaggtca aagcaatatg gactctgaga acacaaggga 4081 tgctcaagga cagtttctta cagtaattgg agcattttgg catctaacaa aggagaggaa 4141 atatatattc tctgtaagaa tggccctagt aaattgcctt aaaactttgc ttgaggctga 4201 tccttattca aaatgggcca ttcttaatgt aatgggaaaa gactttcctg taaatgaagt 4261 atttacacaa tttcttgctg acaatcatca ccaagttcgc atgttggctg cagagtcaat 4321 caatagattg ttccaggaca cgaagggaga ttcttccagg ttactgaaag cacttccttt 4381 gaagcttcag caaacagctt ttgaaaatgc atacttgaaa gctcaggaag gaatgagaga 4441 aatgtcccat agtgctgaga accctgaaac tttggatgaa atttataata gaaaatctgt 4501 tttactgacg ttgatagctg tggttttatc ctgtagccct atctgcgaaa aacaggcttt 4561 gtttgccctg tgtaaatctg tgaaagagaa tggattagaa cctcaccttg tgaaaaaggt 4621 tttagagaaa gtttctgaaa cttttggata tagacgttta gaagacttta tggcatctca 4681 tttagattat ctggttttgg aatggctaaa tcttcaagat actgaataca acttatcttc 4741 ttttcctttt attttattaa actacacaaa tattgaggat ttctatagat cttgttataa 4801 ggttttgatt ccacatctgg tgattagaag tcattttgat gaggtgaagt ccattgctaa 4861 tcagattcaa gaggactgga aaagtcttct aacagactgc tttccaaaga ttcttgtaaa 4921 tattcttcct tattttgcct atgagggtac cagagacagt gggatggcac agcaaagaga 4981 gactgctacc aaggtctatg atatgcttaa aagtgaaaac ttattgggaa aacagattga 5041 tcacttattc attagtaatt taccagagat tgtggtggag ttattgatga cgttacatga 5101 gccagcaaat tctagtgcca gtcagagcac tgacctctgt gacttttcag gggatttgga 5161 tcctgctcct aatccacctc attttccatc gcatgtgatt aaagcaacat ttgcctatat 5221 cagcaattgt cataaaacca agttaaaaag cattttagaa attctttcca aaagccctga 5281 ttcctatcag aaaattcttc ttgccatatg tgagcaagca gctgaaacaa ataatgttta 5341 taagaagcac agaattctta aaatatatca cctgtttgtt agtttattac tgaaagatat 5401 aaaaagtggc ttaggaggag cttgggcctt tgttcttcga gacgttattt atactttgat 5461 tcactatatc aaccaaaggc cttcttgtat catggatgtg tcattacgta gcttctccct 5521 ttgttgtgac ttattaagtc aggtttgcca gacagccgtg acttactgta aggatgctct 5581 agaaaaccat cttcatgtta ttgttggtac acttataccc cttgtgtatg agcaggtgga 5641 ggttcagaaa caggtattgg acttgttgaa atacttagtg atagataaca aggataatga 5701 aaacctctat atcacgatta agcttttaga tccttttcct gaccatgttg tttttaagga 5761 tttgcgtatt actcagcaaa aaatcaaata cagtagagga cccttttcac tcttggagga 5821 aattaaccat tttctctcag taagtgttta tgatgcactt ccattgacaa gacttgaagg 5881 actaaaggat cttcgaagac aactggaact acataaagat cagatggtgg acattatgag 5941 agcttctcag gataatccgc aagatgggat tatggtgaaa ctagttgtca atttgttgca 6001 gttatccaag atggcaataa accacactgg tgaaaaagaa gttctagagg ctgttggaag 6061 ctgcttggga gaagtgggtc ctatagattt ctctaccata gctatacaac atagtaaaga 6121 tgcatcttat accaaggccc ttaagttatt tgaagataaa gaacttcagt ggaccttcat 6181 aatgctgacc tacctgaata acacactggt agaagattgt gtcaaagttc gatcagcagc 6241 tgttacctgt ttgaaaaaca ttttagccac aaagactgga catagtttct gggagattta 6301 taagatgaca acagatccaa tgctggccta tctacagcct tttagaacat caagaaaaaa 6361 gtttttagaa gtacccagat ttgacaaaga aaaccctttt gaaggcctgg atgatataaa 6421 tctgtggatt cctctaagtg aaaatcatga catttggata aagacactga cttgtgcttt 6481 tttggacagt ggaggcacaa aatgtgaaat tcttcaatta ttaaagccaa tgtgtgaagt 6541 gaaaactgac ttttgtcaga ctgtacttcc atacttgatt catgatattt tactccaaga 6601 tacaaatgaa tcatggagaa atctgctttc tacacatgtt cagggatttt tcaccagctg 6661 tcttcgacac ttctcgcaaa cgagccgatc cacaacccct gcaaacttgg attcagagtc 6721 agagcacttt ttccgatgct gtttggataa aaaatcacaa agaacaatgc ttgctgttgt 6781 ggactacatg agaagacaaa agagaccttc ttcaggaaca atttttaatg atgctttctg 6841 gctggattta aattatctag aagttgccaa ggtagctcag tcttgtgctg ctcactttac 6901 agctttactc tatgcagaaa tctatgcaga taagaaaagt atggatgatc aagagaaaag 6961 aagtcttgca tttgaagaag gagcccagag tacaactatt tctagcttga gtgaaaaaag 7021 taaagaagaa actggaataa gtttacagga tcttctctta gaaatctaca gaagtatagg 7081 ggagccagat agtttgtatg gctgtggtgg agggaagatg ttacaaccca ttactagact 7141 acgaacatat gaacacgaag caatgtgggg caaagcccta gtaacatatg acctcgaaac 7201 agcaatcccc tcatcaacac gccaggcagg aatcattcag gccttgcaga atttgggact 7261 ctgccatatt ctttccgtct atttaaaagg attggattat gaaaataaag actggtgtcc 7321 tgaactagaa gaacttcatt accaagcagc atggaggaat atgcagtggg accattgcac 7381 ttccgtcagc aaagaagtag aaggaaccag ttaccatgaa tcattgtaca atgctctaca 7441 atctctaaga gacagagaat tctctacatt ttatgaaagt ctcaaatatg ccagagtaaa 7501 agaagtggaa gagatgtgta agcgcagcct tgagtctgtg tattcgctct atcccacact 7561 tagcaggttg caggccattg gagagctgga aagcattggg gagcttttct caagatcagt 7621 cacacataga caactctctg aagtatatat taagtggcag aaacactccc agcttctcaa 7681 ggacagtgat tttagttttc aggagcctat catggctcta cgcacagtca ttttggagat 7741 cctgatggaa aaggaaatgg acaactcaca aagagaatgt attaaggaca ttctcaccaa 7801 acaccttgta gaactctcta tactggccag aactttcaag aacactcagc tccctgaaag 7861 ggcaatattt caaattaaac agtacaattc agttagctgt ggagtctctg agtggcagct 7921 ggaagaagca caagtattct gggcaaaaaa ggagcagagt cttgccctga gtattctcaa 7981 gcaaatgatc aagaagttgg atgccagctg tgcagcgaac aatcccagcc taaaacttac 8041 atacacagaa tgtctgaggg tttgtggcaa ctggttagca gaaacgtgct tagaaaatcc 8101 tgcggtcatc atgcagacct atctagaaaa ggcagtagaa gttgctggaa attatgatgg 8161 agaaagtagt gatgagctaa gaaatggaaa aatgaaggca tttctctcat tagcccggtt 8221 ttcagatact caataccaaa gaattgaaaa ctacatgaaa tcatcggaat ttgaaaacaa 8281 gcaagctctc ctgaaaagag ccaaagagga agtaggtctc cttagggaac ataaaattca 8341 gacaaacaga tacacagtaa aggttcagcg agagctggag ttggatgaat tagccctgcg 8401 tgcactgaaa gaggatcgta aacgcttctt atgtaaagca gttgaaaatt atatcaactg 8461 cttattaagt ggagaagaac atgatatgtg ggtattccgg ctttgttccc tctggcttga 8521 aaattctgga gtttctgaag tcaatggcat gatgaagaga gacggaatga agattccaac 8581 atataaattt ttgcctctta tgtaccaatt ggctgctaga atggggacca agatgatggg 8641 aggcctagga tttcatgaag tcctcaataa tctaatctct agaatttcaa tggatcaccc 8701 ccatcacact ttgtttatta tactggcctt agcaaatgca aacagagatg aatttctgac 8761 taaaccagag gtagccagaa gaagcagaat aactaaaaat gtgcctaaac aaagctctca 8821 gcttgatgag gatcgaacag aggctgcaaa tagaataata tgtactatca gaagtaggag 8881 acctcagatg gtcagaagtg ttgaggcact ttgtgatgct tatattatat tagcaaactt 8941 agatgccact cagtggaaga ctcagagaaa aggcataaat attccagcag accagccaat 9001 tactaaactt aagaatttag aagatgttgt tgtccctact atggaaatta aggtggacca 9061 cacaggagaa tatggaaatc tggtgactat acagtcattt aaagcagaat ttcgcttagc 9121 aggaggtgta aatttaccaa aaataataga ttgtgtaggt tccgatggca aggagaggag 9181 acagcttgtt aagggccgtg atgacctgag acaagatgct gtcatgcaac aggtcttcca 9241 gatgtgtaat acattactgc agagaaacac ggaaactagg aagaggaaat taactatctg 9301 tacttataag gtggttcccc tctctcagcg aagtggtgtt cttgaatggt gcacaggaac 9361 tgtccccatt ggtgaatttc ttgttaacaa tgaagatggt gctcataaaa gatacaggcc 9421 aaatgatttc agtgcctttc agtgccaaaa gaaaatgatg gaggtgcaaa aaaagtcttt 9481 tgaagagaaa tatgaagtct tcatggatgt ttgccaaaat tttcaaccag ttttccgtta 9541 cttctgcatg gaaaaattct tggatccagc tatttggttt gagaagcgat tggcttatac 9601 gcgcagtgta gctacttctt ctattgttgg ttacatactt ggacttggtg atagacatgt 9661 acagaatatc ttgataaatg agcagtcagc agaacttgta catatagatc taggtgttgc 9721 ttttgaacag ggcaaaatcc ttcctactcc tgagacagtt ccttttagac tcaccagaga 9781 tattgtggat ggcatgggca ttacgggtgt tgaaggtgtc ttcagaagat gctgtgagaa 9841 aaccatggaa gtgatgagaa actctcagga aactctgtta accattgtag aggtccttct 9901 atatgatcca ctctttgact ggaccatgaa tcctttgaaa gctttgtatt tacagcagag 9961 gccggaagat gaaactgagc ttcaccctac tctgaatgca gatgaccaag aatgcaaacg 10021 aaatctcagt gatattgacc agagtttcga caaagtagct gaacgtgtct taatgagact 10081 acaagagaaa ctgaaaggag tggaagaagg cactgtgctc agtgttggtg gacaggtgaa 10141 tttgctcata cagcaggcca tagaccccaa aaatctcagc cgacttttcc caggatggaa 10201 agcttgggtg tgatcttcag tatatgaatt accctttcat tcagccttta gaaattatat 10261 tttagccttt atttttaacc tgccaacata ctttaagtag ggattaatat ttaagtgaac 10321 tattgtgggt ttttttgaat gttggtttta atacttgatt taatcaccac tcaaaaatgt 10381 tttgatggtc ttaaggaaca tctctgcttt cactctttag aaataatggt cattcgggct 10441 gggcgcagcg gctcacgcct gtaatcccag cactttggga ggccgaggtg agcggatcac 10501 aaggtcagga gttcgagacc agcctggcca agagaccagc ctggccagta tggtgaaacc 10561 ctgtctctac taaaaataca aaaattagcc gagcatggtg gcgggcacct gtagtcccag 10621 ctactcgagc atgcatctag agggccctat tctatagtgt cacctaaatg ctagagctcg 10681 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt 10741 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat 10801 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag 10861 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc 10921 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg 10981 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 11041 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 11101 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 11161 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 11221 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 11281 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat 11341 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg 11401 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg 11461 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 11521 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 11581 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 11641 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag 11701 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc 11761 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga 11821 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa 11881 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt 11941 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg 12001 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa 12061 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac 12121 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt 12181 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact 12241 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg 12301 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg 12361 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc 12421 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt 12481 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc 12541 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg 12601 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg 12661 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 12721 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta 12781 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 12841 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 12901 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 12961 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 13021 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 13081 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 13141 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 13201 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 13261 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 13321 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 13381 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 13441 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 13501 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 13561 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 13621 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 13681 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 13741 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 13801 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 13861 aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 13921 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 13981 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 14041 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 14101 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 14161 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 14221 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 14281 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 14341 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 14401 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 14461 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 14521 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 14581 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 14641 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 14701 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 14761 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 14821 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 14881 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 14941 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 15001 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 15061 cgcgcacatt tccccgaaaa gtgccacctg acgtc //