LOCUS Exported 9263 bp ds-DNA circular SYN 12-JUL-2022 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS gag/pol SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9263) AUTHORS Reya T, Duncan AW, Ailles L, Domen J, Scherer DC, Willert K, Hintz L, Nusse R, Weissman IL TITLE A role for Wnt signalling in self-renewal of haematopoietic stem cells. JOURNAL Nature. 2003 May 22. 423(6938):409-14. PUBMED 12717450 REFERENCE 2 (bases 1 to 9263) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 12, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9263 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(28..888) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 651..670 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(889..993) /gene="bla" /label=AmpR promoter primer_bind 1061..1079 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(1117..1139) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 1452..1474 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 1466..1483 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 1467..1483 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 1843..2104 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" intron 2150..2246 /label=SV40 intron /note="modified SV40 intron with splice donor and acceptor sites" regulatory 2307..2316 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2313..7529 /codon_start=1 /gene="gag-pol" /product="Moloney murine leukemia virus Gag-Pol polyprotein" /label=gag-pol /note="contains a read-through stop codon" /translation="MGQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPT FNVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPK PPPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGAKPKPQVLSDSGGPLIDLLTEDPP PYRDPRPPPSDRDGNGGEATPAGEAPDPSPMASRLRGRREPPVADSTTSQAFPLRAGGN GQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGTLLTGE EKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYRQLLLAG LQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETNVSMSFIW QSAPDIGRKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERIRRETEEKEERRRTE DEQKEKERDRRRHREMSKLLATVVSGQKQDRQGGERRRSQLDRDQCAYCKEKGHWAKDC PKKPRGPRGPRPQTSLLTLDD*GGQGQEPPPEPRITLKVGGQPVTFLVDTGAQHSVLTQ NPGPLSDKSAWVQGATGGKRYRWTTDRKVHLATGKVTHSFLHVPDCPYPLLGRDLLTKL KAQIHFEGSGAQVMGPMGQPLQVLTLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAE TGGMGLAVRQAPLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPW NTPLLPVKKPGTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKD AFFCLRLHPTSQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFDEALHRDLADFRI QHPDLILLQYVDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGY LLKEGQRWLTEARKETVMGQPTPKTPRQLREFLGTAGFCRLWIPGFAEMAAPLYPLTKT GTLFNWGPDQQKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWR RPVAYLSKKLDPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPP DRWLSNARMTHYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRP DLTDQPLPDADHTWYTDGSSLLQEGQRKAGAAVTTETEVIWAKALPAGTSAQRAELIAL TQALKMAEGKKLNVYTDSRYAFATAHIHGEIYRRRGLLTSEGKEIKNKDEILALLKALF LPKRLSIIHCPGHQKGHSAEARGNRMADQAARKAAITETPDTSTLLIENSSPYTSEHFH YTVTDIKDLTKLGAIYDKTKKYWVYQGKPVMPDQFTFELLDFLHQLTHLSFSKMKALLE RSHSPYYMLNRDRTLKNITETCKACAQVNASKSAVKQGTRVRGHRPGTHWEIDFTEIKP GLYGYKYLLVFIDTFSGWIEAFPTKKETAKVVTKKLLEEIFPRFGMPQVLGTDNGPAFV SKVSQTVADLLGIDWKLHCAYRPQSSGQVERMNRTIKETLTKLTLATGSRDWVLLLPLA LYRARNTPGPHGLTPYEILYGAPPPLVNFPDPDMTRVTNSPSLQAHLQALYLVQHEVWR PLAAAYQEQLDRPVVPHPYRVGDTVWVRRHQTKNLEPRWKGPYTVLLTTPTALKVDGIA AWIHAAHVKAADPGGGPSSRLTWRVQRSQNPLKIRLTREAP" primer_bind 2662..2684 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 2700..2716 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" misc_feature 7092..7466 /label=pol region /note="Moloney murine leukemia virus (MMLV) pol region containing the splice acceptor site" primer_bind 7404..7420 /label=pBMN 5' /note="MMLV sequence, for inserts in pBMN retroviral vector" polyA_signal 7544..7678 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7568..7587) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 7622..7641 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(7789..7805) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7789..7805) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7802..7824) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7813..7829 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7837..7867) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7882..7903 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8020..8037) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8191..8779) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8271..8290) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 61 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 121 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 181 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 241 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 301 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 361 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 421 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 481 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 541 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 601 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 661 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 721 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 781 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 841 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 901 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 961 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 1021 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 1081 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 1141 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 1201 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 1261 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 1321 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 1381 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 1441 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaatttc gaccaattct 1501 catgtttgac agcttatcat cgcagatccg tatggtgcac tctcagtaca atctgctctg 1561 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 1621 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 1681 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgtatct 1741 gaggggacta gggtgtgttt aggcgaaaag cggggcttcg gttgtacgcg gttaggagtc 1801 ccctcaggat atagtagttt cgcttttgca tagggagggg gaaatgtagt cttatgcaat 1861 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa 1921 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag 1981 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttccgcattg cagagatatt 2041 gtatttaagt gcctagctcg atacaataaa cgccatttga ccattcacca cattggtgtg 2101 cacctccaag ctgggtactc gaggaactga aaaaccagaa agttaactgg taagtttagt 2161 ctttttgtct tttatttcag gtcccggatc cggtggtggt gcaaatcaaa gaactgctcc 2221 tcagtggacg ttgcctttac ttctaggcct gtacggaagt gttacttctg ctctaaaagc 2281 tgcggaattg tacccgcggc cgcgccgcca ccatgggcca gactgttacc actcccttaa 2341 gtttgacctt aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg 2401 tcaagaagag acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat 2461 ggccgcgaga cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt 2521 cacctggccc gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg 2581 cttttgaccc ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc 2641 ctccatccgc cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc 2701 tttatccagc cctcactcct tctctaggcg ccaaacctaa acctcaagtt ctttctgaca 2761 gtggggggcc gctcatcgac ctacttacag aagacccccc gccttatagg gacccaagac 2821 cacccccttc cgacagggac ggaaatggtg gagaagcgac ccctgcggga gaggcaccgg 2881 acccctcccc aatggcatct cgcctacgtg ggagacggga gccccctgtg gccgactcca 2941 ctacctcgca ggcattcccc ctccgcgcag gaggaaacgg acagcttcaa tactggccgt 3001 tctcctcttc tgacctttac aactggaaaa ataataaccc ttctttttct gaagatccag 3061 gtaaactgac agctctgatc gagtctgttc tcatcaccca tcagcccacc tgggacgact 3121 gtcagcagct gttggggact ctgctgaccg gagaagaaaa acaacgggtg ctcttagagg 3181 ctagaaaggc ggtgcggggc gatgatgggc gccccactca actgcccaat gaagtcgatg 3241 ccgcttttcc cctcgagcgc ccagactggg attacaccac ccaggcaggt aggaaccacc 3301 tagtccacta tcgccagttg ctcctagcgg gtctccaaaa cgcgggcaga agccccacca 3361 atttggccaa ggtaaaagga ataacacaag ggcccaatga gtctccctcg gccttcctag 3421 agagacttaa ggaagcctat cgcaggtaca ctccttatga ccctgaggac ccagggcaag 3481 aaactaatgt gtctatgtct ttcatttggc agtctgcccc agacattggg agaaagttag 3541 agaggttaga agatttaaaa aacaagacgc ttggagattt ggttagagag gcagaaaaga 3601 tctttaataa acgagaaacc ccggaagaaa gagaggaacg tatcaggaga gaaacagagg 3661 aaaaagaaga acgccgtagg acagaggatg agcagaaaga gaaagaaaga gatcgtagga 3721 gacatagaga gatgagcaag ctattggcca ctgtcgttag tggacagaaa caggatagac 3781 agggaggaga acgaaggagg tcccaactcg atcgcgacca gtgtgcctac tgcaaagaaa 3841 aggggcactg ggctaaagat tgtcccaaga aaccacgagg acctcgggga ccaagacccc 3901 agacctccct cctgacccta gatgactagg gaggtcaggg tcaggagccc ccccctgaac 3961 ccaggataac cctcaaagtc ggggggcaac ccgtcacctt cctggtagat actggggccc 4021 aacactccgt gctgacccaa aatcctggac ccctaagtga taagtctgcc tgggtccaag 4081 gggctactgg aggaaagcgg tatcgctgga ccacggatcg caaagtacat ctagctaccg 4141 gtaaggtcac ccactctttc ctccatgtac cagactgtcc ctatcctctg ttaggaagag 4201 atttgctgac taaactaaaa gcccaaatcc actttgaggg atcaggagct caggttatgg 4261 gaccaatggg gcagcccctg caagtgttga ccctaaatat agaagatgag tatcggctac 4321 atgagacctc aaaagagcca gatgtttctc tagggtccac atggctgtct gattttcctc 4381 aggcctgggc ggaaaccggg ggcatgggac tggcagttcg ccaagctcct ctgatcatac 4441 ctctgaaagc aacctctacc cccgtgtcca taaaacaata ccccatgtca caagaagcca 4501 gactggggat caagccccac atacagagac tgttggacca gggaatactg gtaccctgcc 4561 agtccccctg gaacacgccc ctgctacccg ttaagaaacc agggactaat gattataggc 4621 ctgtccagga tctgagagaa gtcaacaagc gggtggaaga catccacccc accgtgccca 4681 acccttacaa cctcttgagc gggctcccac cgtcccacca gtggtacact gtgcttgatt 4741 taaaggatgc ctttttctgc ctgagactcc accccaccag tcagcctctc ttcgcctttg 4801 agtggagaga tccagagatg ggaatctcag gacaattgac ctggaccaga ctcccacagg 4861 gtttcaaaaa cagtcccacc ctgtttgatg aggcactgca cagagaccta gcagacttcc 4921 ggatccagca cccagacttg atcctgctac agtacgtgga tgacttactg ctggccgcca 4981 cttctgagct agactgccaa caaggtactc gggccctgtt acaaacccta gggaacctcg 5041 ggtatcgggc ctcggccaag aaagcccaaa tttgccagaa acaggtcaag tatctggggt 5101 atcttctaaa agagggtcag agatggctga ctgaggccag aaaagagact gtgatggggc 5161 agcctactcc gaagacccct cgacaactaa gggagttcct agggacggca ggcttctgtc 5221 gcctctggat ccctgggttt gcagaaatgg cagccccctt gtaccctctc accaaaacgg 5281 ggactctgtt taattggggc ccagaccaac aaaaggccta tcaagaaatc aagcaagctc 5341 ttctaactgc cccagccctg gggttgccag atttgactaa gccctttgaa ctctttgtcg 5401 acgagaagca gggctacgcc aaaggtgtcc taacgcaaaa actgggacct tggcgtcggc 5461 cggtggccta cctgtccaaa aagctagacc cagtagcagc tgggtggccc ccttgcctac 5521 ggatggtagc agccattgcc gtactgacaa aggatgcagg caagctaacc atgggacagc 5581 cactagtcat tctggccccc catgcagtag aggcactagt caaacaaccc cccgaccgct 5641 ggctttccaa cgcccggatg actcactatc aggccttgct tttggacacg gaccgggtcc 5701 agttcggacc ggtggtagcc ctgaacccgg ctacgctgct cccactgcct gaggaagggc 5761 tgcaacacaa ctgccttgat atcctggccg aagcccacgg aacccgaccc gacctaacgg 5821 accagccgct cccagacgcc gaccacacct ggtacacgga tggaagcagt ctcttacaag 5881 agggacagcg taaggcggga gctgcggtga ccaccgagac cgaggtaatc tgggctaaag 5941 ccctgccagc cgggacatcc gctcagcggg ctgaactgat agcactcacc caggccctaa 6001 agatggcaga aggtaagaag ctaaatgttt atactgatag ccgttatgct tttgctactg 6061 cccatatcca tggagaaata tacagaaggc gtgggttgct cacatcagaa ggcaaagaga 6121 tcaaaaataa agacgagatc ttggccctac taaaagccct ctttctgccc aaaagactta 6181 gcataatcca ttgtccagga catcaaaagg gacacagcgc cgaggctaga ggcaaccgga 6241 tggctgacca agcggcccga aaggcagcca tcacagagac tccagacacc tctaccctcc 6301 tcatagaaaa ttcatcaccc tacacctcag aacattttca ttacacagtg actgatataa 6361 aggacctaac caagttgggg gccatttatg ataaaacaaa gaagtattgg gtctaccaag 6421 gaaaacctgt gatgcctgac cagtttactt ttgaattatt agactttctt catcagctga 6481 ctcacctcag cttctcaaaa atgaaggctc tcctagagag aagccacagt ccctactaca 6541 tgctgaaccg ggatcgaaca ctcaaaaata tcactgagac ctgcaaagct tgtgcacaag 6601 tcaacgccag caagtctgcc gttaaacagg gaactagggt ccgcgggcat cggcccggca 6661 ctcattggga gatcgatttc accgagataa agcccggatt gtatggctat aaatatcttc 6721 tagtttttat agataccttt tctggctgga tagaagcctt cccaaccaag aaagaaaccg 6781 ccaaggtcgt aaccaagaag ctactagagg agatcttccc caggttcggc atgcctcagg 6841 tattgggaac tgacaatggg cctgccttcg tctccaaggt gagtcagaca gtggccgatc 6901 tgttggggat tgattggaaa ttacattgtg catacagacc ccaaagctca ggccaggtag 6961 aaagaatgaa tagaaccatc aaggagactt taactaaatt aacgcttgca actggctcta 7021 gagactgggt gctcctactc cccttagccc tgtaccgagc ccgcaacacg ccgggccccc 7081 atggcctcac cccatatgag atcttatatg gggcaccccc gccccttgta aacttccctg 7141 accctgacat gacaagagtt actaacagcc cctctctcca agctcactta caggctctct 7201 acttagtcca gcacgaagtc tggagacctc tggcggcagc ctaccaagaa caactggacc 7261 gaccggtggt acctcaccct taccgagtcg gcgacacagt gtgggtccgc cgacaccaga 7321 ctaagaacct agaacctcgc tggaaaggac cttacacagt cctgctgacc acccccaccg 7381 ccctcaaagt agacggcatc gcagcttgga tacacgccgc ccacgtgaag gctgccgacc 7441 ccgggggtgg accatcctct agactgacat ggcgcgttca acgctctcaa aaccccttaa 7501 aaataaggtt aacccgcgag gccccctaat gagcggccgc ggggatccag acatgataag 7561 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg 7621 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa 7681 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggttttttc 7741 ggatcctcta gagtcgacct gcaggcatgc aagcttggcg taatcatggt catagctgtt 7801 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 7861 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 7921 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 7981 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 8041 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 8101 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 8161 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 8221 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 8281 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 8341 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 8401 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 8461 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 8521 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 8581 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 8641 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 8701 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 8761 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 8821 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 8881 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 8941 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 9001 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 9061 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 9121 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 9181 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 9241 tttgcgcaac gttgttgcca ttg //