LOCUS       Exported                 900 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCDNA3-Flag MKK6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Raingeaud J, Whitmarsh AJ, Barrett T, Derijard B, Davis RJ
  TITLE     MKK3- and MKK6-regulated gene expression is mediated by the p38 
            mitogen-activated protein kinase signal transduction pathway.
  JOURNAL   Mol Cell Biol. 1996 Mar . 16(3):1247-55.
  PUBMED    8622669
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             103..126
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgngggnttn cgactctctg gctactgaga cccactgctt actggcttat cgaaattaat
       61 acgactcact atagggagac ccaagcttcg gcagccaaca tggactataa ggacgatgat
      121 gacaaatctc agtcgaaagg caagaagcga aaccctggcc ttaaaattcc aaaagaagca
      181 tttgaacaac ctcagaccag ttccacacca cctagagatt tagactccaa ggcttgcatt
      241 tctattggaa atcagaactt tgaggtgaag gcagatgacc tggagcctat aatggaactg
      301 ggacgaggtg cgtacggggt ggtggagaag atgcggcacg tgcccagcgg gcagatcatg
      361 gcagtgaagc ggatccgagc cacagtaaat agccaggaac agaaacggct actgatggat
      421 ttggatattt ccatgaggac ggtggactgt ccattcactg tcacctttta tggcgcactg
      481 tttcgggagg gtgatgtgtg gatctgcatg gagctcatgg atacatcact agataaattc
      541 tacaaacaag ttattgataa aggccagaca attccagagg acatcttagg gaaaatagca
      601 gtttctattg taaaagcatt agaacattta catagtaagc tgtctgtcat tcacagagac
      661 gtcaagcctt ctaatgtact catcaatgct ctcggtcaag tgaagatgtg cgattttgga
      721 atcagtggct acttggtgga ctctgttgct aaaacaattg atgcaggttg caaaccatac
      781 atggcccctg aaagaataaa cccagagctc aaccagaagg gatacagtgt gaagtctgac
      841 atttggagtc tgggcatcac gatgattgag ttggccatcc tcatcttatc nggatcntcc
//
