LOCUS       Exported                6527 bp ds-DNA     circular SYN 18-JUL-2022
DEFINITION  Ubiquitous expression of constitutively active Retinoic Acid 
            Receptor alpha.
ACCESSION   .
VERSION     .
KEYWORDS    CAG-RARa-VP16
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Amamoto R, Wallick GK, Cepko CL
  TITLE     Retinoic acid signaling mediates peripheral cone photoreceptor 
            survival in a mouse model of retina degeneration.
  JOURNAL   Elife. 2022 Mar 22;11. pii: 76389. doi: 10.7554/eLife.76389.
  PUBMED    35315776
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 18, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     intron          122..1138
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1062..1084
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1146..1165
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1188..1197
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2730..2963
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(3048..3067)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3113..3168
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3167..3186)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3529..3545)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3529..3545)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3542..3564)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3553..3569
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3577..3607)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3622..3643
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3702..3897
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3748..3883
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3810..3829
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    3903..4037
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3940..3959)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3994..4013
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4105..4122)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4276..4864)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4356..4375)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5035..5895)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5658..5677
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5896..6000)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6031..6410
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
       61 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      121 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      181 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      241 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      301 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      361 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      421 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
      481 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
      541 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
      601 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
      661 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
      721 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
      781 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
      841 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
      901 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
      961 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1021 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1081 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1141 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaaggcc accatggcca
     1201 gcaatagcag ttcctgccca acacctgggg gcgggcacct caatgggtac ccagtacccc
     1261 cctacgcctt cttctttccc cctatgctgg gtggactctc cccacccggc gctctcacca
     1321 gcctccagca ccagcttcca gtcagtggtt acagcacacc gtccccagcc accatcgaga
     1381 cccagagcag cagttccgaa gagatagtac ccagccctcc ctcaccaccg cccctgcccc
     1441 gcatctacaa gccttgcttt gtttgtcaag acaaatcatc cggctaccac tatggggtca
     1501 gcgcctgtga gggctgtaag ggcttcttcc gacgaagcat ccagaagaac atggtgtata
     1561 cgtgtcaccg ggacaagaac tgcatcatca acaaggtgac ccggaaccgc tgccagtact
     1621 gccggctgca gaaatgtttc gacgtgggca tgtccaagga gtcggtgcga aacgatcgaa
     1681 acaaaaagaa gaaagaggca cccaagcccg agtgctcaga gagctacacg ctgacgcctg
     1741 aggtgggcga gctcattgag aaggttcgca aagcgcacca ggagaccttc ccggccctct
     1801 gccagctggg caagtacact acgaacaaca gctcagaaca acgagtctcc ctggacattg
     1861 acctctggga caagttcagt gaactctcca ccaagtgcat cattaagact gtggagttcg
     1921 ccaagcagct tcccggcttc accaccctca ccatcgccga ccagatcacc ctcctcaagg
     1981 ctgcctgcct ggatatcctg attctgcgaa tctgcacgcg gtacacgcct gagcaagaca
     2041 caatgacctt ctcagatgga ctgaccctga accggactca gatgcacaac gctggctttg
     2101 gccccctcac cgacttggtc tttgccttcg ccaaccagct gctgcccctg gagatggacg
     2161 atgctgagac tggactgctc agtgccatct gcctcatctg tggagaccga caggacctgg
     2221 agcagccaga caaggtggac atgctgcaag agccgctgct ggaagcactg aaagtctacg
     2281 tccggaaacg gaggcccagc cgaccccaca tgttccccaa gatgctgatg aagatcacag
     2341 accttcggag catcagcgcc aagggagctg aacgggtgat cacattgaag atggagatcc
     2401 caggctccat gccaccgctg atccaggaaa tgctggagaa ctctgagggc ttggacactc
     2461 taagcggaca gtcggggggc ggaacacgag atgggggtgg cctggccccc cctccgggta
     2521 gctgtagccc cagcctcagt cccagctccc acagaagcag cccagccacc caatccccag
     2581 gaccggtccg cgcgcgtacg aaaaacaatt acgggtctac catcgagggc ctgctcgatc
     2641 tcccggacga cgacgccccc gaagaggcgg ggctggcggc tccgcgcctg tcctttctcc
     2701 ccgcgggaca cacgcgcaga ctgtcgacgg cccccccgac cgatgtcagc ctgggggacg
     2761 agctccactt agacggcgag gacgtggcga tggcgcatgc cgacgcgcta gacgatttcg
     2821 atctggacat gttgggggac ggggattccc cgggtccggg atttaccccc cacgactccg
     2881 ccccctacgg cgctctggat atggccgact tcgagtttga gcagatgttt accgatgccc
     2941 ttggaattga cgagtacggt gggtaggcgc actcctcagg tgcaggctgc ctatcagaag
     3001 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct
     3061 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
     3121 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
     3181 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
     3241 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
     3301 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
     3361 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
     3421 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
     3481 ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt
     3541 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     3601 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     3661 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca
     3721 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3781 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     3841 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     3901 ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3961 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4021 cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4081 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4141 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4201 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4261 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4321 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4381 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4441 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4501 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4561 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4621 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4681 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     4741 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4801 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4861 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4921 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4981 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5041 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5101 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     5161 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     5221 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     5281 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     5341 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     5401 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     5461 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     5521 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     5581 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     5641 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     5701 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     5761 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     5821 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     5881 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     5941 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6001 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt
     6061 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     6121 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     6181 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     6241 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     6301 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     6361 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
     6421 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
     6481 tatttatttt ttaattattt tgtgcagcga tgggggcggg ggggggg
//
